Chiang Mai Journal of Science

Effect of NaCl Concentration on Production of Actinomycin-D by Streptomyces sp. Strain J6.2 Isolated from Thailand

Paper Type	Contributed Paper
Title	Effect of NaCl Concentration on Production of Actinomycin-D by Streptomyces sp. Strain J6.2 Isolated from Thailand
Author	Nattachai Kengpipat [a], Surachai Pornpakakul*[b], Jittra Piapukiew [c], Thanapat Palaga [d], Geoff Robson [e], Anthony J.S. Whalley [d] and Prakitsin Sihanonth**[d]
Email	psuracha@chula.ac.th; sprakits@chula.ac.th
Abstract:  Antimicrobial actinomycetes were screened from mangrove soil in Thailand. Streptomyces sp. J6.2 which had high activity and broad spectrum was selected to study it active metabolite. Morphology and physiology of Strain J6.2 indicated a Streptomyces sp. The 16S rDNA sequence for 1,401 bp is closely related to S. parvulus (100%). A bioactive compound produced by Strain 6.2 was characterized by 2D NMR including COSY, HSQC and HMBC as actinomycin-D. The specific productivity of actinomycin-D was optimized by varying NaCl concentration in culture media. High actinomycin-D yields were obtained in sodium caseinate broth supplemented with 20 g/l malt extract and 20g/l NaCl (80.39 ± 0.72 mg/l).
Start & End Page	683 - 687
Received Date	2014-05-13
Revised Date
Accepted Date	2015-05-21
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Keyword	Streptomyces parvulus, actinomycin-D, salt stress, specific productivity
Volume	Vol.43 No.3 (APRIL 2016)
DOI
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