Effect of NaCl Concentration on Production of Actinomycin-D by Streptomyces sp. Strain J6.2 Isolated from Thailand
Nattachai Kengpipat [a], Surachai Pornpakakul*[b], Jittra Piapukiew [c], Thanapat Palaga [d], Geoff Robson [e], Anthony J.S. Whalley [d] and Prakitsin Sihanonth**[d]* Author for corresponding; e-mail address: psuracha@chula.ac.th; sprakits@chula.ac.th
Volume: Vol.43 No.3 (APRIL 2016)
Research Article
DOI:
Received: 13 May 2014, Revised: -, Accepted: 21 May 2015, Published: -
Citation: Kengpipat N., Pornpakakul S., Piapukiew J., Palaga T., Robson G., Whalley A.J., et al., Effect of NaCl Concentration on Production of Actinomycin-D by Streptomyces sp. Strain J6.2 Isolated from Thailand, Chiang Mai Journal of Science, 2016; 43(3): 683-687.
Abstract
Antimicrobial actinomycetes were screened from mangrove soil in Thailand. Streptomyces sp. J6.2 which had high activity and broad spectrum was selected to study it active metabolite. Morphology and physiology of Strain J6.2 indicated a Streptomyces sp. The 16S rDNA sequence for 1,401 bp is closely related to S. parvulus (100%). A bioactive compound produced by Strain 6.2 was characterized by 2D NMR including COSY, HSQC and HMBC as actinomycin-D. The specific productivity of actinomycin-D was optimized by varying NaCl concentration in culture media. High actinomycin-D yields were obtained in sodium caseinate broth supplemented with 20 g/l malt extract and 20g/l NaCl (80.39 ± 0.72 mg/l).