Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Anatomical Characteristics of Leaf and Petiole and Presence of Calcium Oxalate Crystals in Giant Elephant Ear (Colocasia gigantea (Blume) Hook. f.)

Yupadee Paopun, Piyanan Thanomchat and Nustsaba Kronburee
* Author for corresponding; e-mail address: rdiydp@ku.ac.th
Volume: Vol.52 No.2 (March 2025)
Research Article
DOI: https://doi.org/10.12982/CMJS.2025.016
Received: 28 August 2024, Revised: 22 January 2025, Accepted: 26 Febuary 2025, Published: 24 March 2025

Citation: Paopun Y., Thanomchat P. and Kronburee N., Anatomical characteristics of leaf and petiole and presence of calcium oxalate crystals in giant elephant ear (Colocasia gigantea (Blume) Hook. f.). Chiang Mai Journal of Science, 2025; 52(2): e2025016. DOI 10.12982/CMJS.2025.016.

Graphical Abstract

Graphical Abstract

Abstract

     The genus Colocasia contains mostly calcium oxalate (CaOX), especially found in petioles and leaves. The petioles of C. gigantea are usually consumed by Southeast Asian people for cooking. In addition, fresh petioles together with leaves are utilized as animal feed. This study aims to determine the anatomical characteristics of both parts of the above mentioned to identify characters of CaOX crystals using a compound light microscope, polarized microscope, scanning electron microscope, and energy-dispersive X-ray spectrometer. The results showed that the leaf anatomical characteristics comprise four layers, i.e. the upper and the lower epidermis, mesophyll, and vascular bundles, respectively. The mesophyll layer consists of palisade cells, spongy cells, and air spaces. Besides the idioblast cells also found in the mesophyll. The petiole comprises three layers, i.e. the epidermis, stele (vascular bundle), and cortex where parenchyma cells, air spaces, and idioblast cells exist. The idioblast cells contain a group of raphide (or raphide bundle) of CaOX in the cell. The palisade cells of the leaf and parenchyma cells of the petiole contain other shapes of CaOX crystals such as druse, rectangular prism, hexagonal prism, and octahedron prism. The length and thickness of the raphide crystals bundle are 60.40-67.50 μm and 26.00-30.90 μm, respectively. The length and thickness of needle-like crystals are 20.80- 67.30 μm and 0.86-1.04 μm, respectively. The diameter of crystals in a druse form is 25.70-30.60 μm. The small crystals appear in three forms, i.e. rectangular, hexagonal, and octagonal prism. The length, width, and thickness of crystals are 3.57-6.57 μm, 1.11-2.05 μm, and 0.33-2.61 μm, respectively. The EDS X-ray spectra and maps analyzed from the crystals found the elements of calcium (Ca) and oxygen (O).

Keywords: giant elephant ear, Colocasia gigantea, anatomical, calcium oxalate, SEM, LM, EDS, X-ray mapping

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Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
page: 1 - 16

Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A

Vol.42 No.1 (JANUARY 2015)
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Exact Solution for Average Run Length of CUSUM Charts for MA(1) Process
page: 1449 - 1456

Kanita Petcharat[a], Yupaporn Areepong*[a], Saowanit Sukparungsee[a] and Gabriel Mititelu[b]

Vol.41 No.5/2 (OCTOBER 2014)
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In vitro Study on antifungal activity of Achillea wilhelmsii flower essential oil against twenty strains of Candida albicans
page: 1058 - 1064

Leila Amjad*[a], Kobra Mousavidehmourdi [b], Zahra Rezvani [c]

Vol.41 No.5/1 (OCTOBER 2014)
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Ideals in Quotient Semirings
page: 77 - 82

Shahabaddin Ebrahimi Atani and Ameneh Gholamalipour Garfami

Vol.40 No.1 (JANUARY 2013)
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Low threshold voltage pentacene OTFTs with La2O3 gate insulating layer using TSD
page: 263 - 269

P. K. Saikia [a], U.J. Mahanta[c], P. Saikia*[a], B. Baishya[a], R Sarma[b] & D. Saikia [a,b,c],

Vol.39 No.2 (APRIL 2012)
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Analysis of Average Run Length for CUSUM Procedure with Negative Exponential Data
page: 200 - 208

Jaruchat Busaba, Saowanit Sukparungsee, Yapaporn Areepong and Gabriel Mititelu

Vol.39 No.2 (APRIL 2012)
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Capturing Knowledge for Interpretation based on Surrounding words of Consecutive Serial Verb Constructions
page: 139 - 161

Pakawan Pugsee, Wanchai Rivepiboon and Pattarasinee Bhattanakosol

Vol.39 No.2 (APRIL 2012)
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Effect of Relative Humidity on MechanicalProperties of Blended Chitosan-Methylcellulose Film
page: 133 - 137

Pornchai Rachtanapun and Panchat Wongchaiya

Vol.39 No.1 (JANUARY 2012)
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Low Threshold Voltage Pentacene OTFTs with O2 Annealed Pr2O3 Gate Insulating Layer
page: 653 - 657

Prasanta Kumar Saikia, Puja Saikia, Ranjit Sarma and Dipok Saikia

Vol.38 No.4 (OCTOBER 2011)
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Preparing Activated Carbon from Palm Shell for Biodiesel Fuel Production
page: 572 - 578

Achanai Buasri, Nattawut Chaiyut and Chaiwut Nakweang

Vol.38 No.4 (OCTOBER 2011)
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Almost Primal Ideals in Commutative Rings
page: 161 - 165

Ahmad Y. Darani

Vol.38 No.2 (APRIL 2011)
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Electrodeposition of Nickel Sulfide Thin Films Using Triethanolamine as a Complexing Agent
page: 131 - 137

Anuar Kassim*, Zulkarnain Zainal, Saravanan Nagalingam, Dzulkefly Kuang and Nor Hamizi Sharafaddin

Vol.31 No.2 May 2004
Research Article View: 941 Download: 236
Performance of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man) Reared in Earthen Ponds Beneath Plastic Film Shelters
page: 89 - 96

Niwooti Whangchai, Thepparath Ungsethaphand, Chanagun Chitmanat, Kriangsak Mengumphan and Supattra

Vol.34 No.1 (JANUARY 2007)
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FESEM Analysis of BPSG Films After Reflow
page: 35 - 46

Uda Hashim, Nik H.N. Hamat, and Ramzan M. Ayab

Vol.34 No.1 (JANUARY 2007)
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Analysis of Intact Glucosinolates in Thai Broccoli Seeds
page: 223 - 230

Sarunya Chuanphongpanich*[a], Duang Buddhasukh [b], Pichai Pirakitikulr [b] and Sukon Phanichphant

Vol.33 No.2 (MAY 2006)
Research Article View: 943 Download: 354
Antioxidant Capacity of Broccoli Seeds Grown in Thailand
page: 117 - 122

Sarunya Chuanphongpanich* [a], Maitree Suttajit [b], Sukon Phanichphant [c Duang Buddhasukh [c]

Vol.33 No.1 (JANUARY 2006)
Research Article View: 1,035 Download: 367
Stanol Synthesis from Palm Oil Distillate
page: 109 - 116

Sarunya Chuanphongpanich* [a], Nipon T uwanon [a], Duang Buddhasukh [b], Pichai Pirakitik

Vol.33 No.1 (JANUARY 2006)
Research Article View: 961 Download: 314
Pressure-Temperature Estimates of the Metamorphosed Sedimentary Rocks on the Eastern Side of Khao Phai, Immediate West of Ban Choeng Chai, Wang Pong, Phetchabun Province, Thailand
page: 283 - 293

Yuenyong Panjasawatwong, Boontarika Srithai and Udomporn Wipakul

Vol.35 No.2 (MAY 2008)
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Thermoelectric P Proper roper roperties ties of Antimony T Telluride elluride Thin Films ilms Deposited using R R..F F. . Magnetron Sputtering
page: 459 - 464

Kandasamy andasamy andasamy, , S S*., ., P Pachoud, achoud, D., Holland, A., K Kalantar alantar alan

Vol.32 No.3 (SEPTEMBER 2005)
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Urbach Energy of Hexagonal ZnO and Mg 0.05 Zn 0.95 O Thin Film Grown by RF Sputtering
page: 453 - 458

Sornchai T anunchai, Sripen T owta, Nik orn Mangk orntong, P ongsri Mangk orntong and Supab

Vol.32 No.3 (SEPTEMBER 2005)
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Dynamic Pyroelectric Properties of La-Doped PbTiO 3 Thin F ilms with V arious L a Concentrations
page: 343 - 349

Yung Sup Y oon

Vol.32 No.3 (SEPTEMBER 2005)
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Effect of Deposition Time Towards the Properties of Nickel Sulfide Thin Films Prepared in the Presence of Sodium Tartrate as a Complexing Agent
page: 121 - 126

Anuar Kassim* [a], Zulkarnain Zainal [a], Saravanan Nagalingam [b] and Zuriyatina Abdullah [a]

Vol.32 No.2 (MAY 2005)
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Synthesis of Cocoa Butter Equivalent from Palm Oil by Carica papaya Lipase-Catalyzed Interesterification
page: 359 - 368

Porntippa Pinyaphong, and Suree Phutrakul

Vol.36 No.3 (SEPTEMBER 2009)
Research Article View: 1,501 Download: 276
Methods for Modeling Incidence Rates with Application to Pneumonia Among Children in Surat Thani Province, Thailand
page: 29 - 38

Noodchanath Kongchouy, Chamnein Choonpradub, and Metta Kuning

Vol.37 No.1 (JANUARY 2010)
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Co-Semisimple Modules and Multiplication Modules
page: 31 - 33

Phannika Leksing and Jintana Sanwong*

Vol.29 No.1 (APRIL 2002)
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Sintering Behaviour of Lanthanum Magnesium Niobate Ceramics
page: 125 - 130

Supon Ananta

Vol.28 No.2 (DECEMBER 2001)
Research Article View: 920 Download: 288
Retention Behavior of Aromatic Amines with Some Ionic Liquids Mobile Phase in Semi-micro HPLC
page: 384 - 396

Yuppadee Nusai, Hitoshi Koizumi, Masaki Tachibana, Kazue Tani, and Nobutoshi Kiba

Vol.37 No.3 (SEPTEMBER 2010)
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