Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Partitioning of Bromelain from Pineapple Stem (Smooth Cayenne) by Aqueous Two Phase System and Its Application for Recovery and Purification of Polyhydroxyalkanoate

Kanokphorn Sangkharak *[a], Piyaporn Wangsirikul [a,b], Nisa Pichid [a], Tewan Yunu [a] and Poonsuk
* Author for corresponding; e-mail address: skanokphorn@yahoo.com
Volume: Vol.43 No.4 (JULY 2016)
Research Article
DOI:
Received: 29 April 2015, Revised: -, Accepted: 7 September 2015, Published: -

Citation: Sangkharak K., Wangsirikul P., Pichid N., Yunu T. and Poonsuk , Partitioning of Bromelain from Pineapple Stem (Smooth Cayenne) by Aqueous Two Phase System and Its Application for Recovery and Purification of Polyhydroxyalkanoate , Chiang Mai Journal of Science, 2016; 43(4): 794-807.

Abstract

 Bromelain from pineapple stem was extracted using aqueous two phase system (ATPs). In addition, the application of bromelain for recovery and purification of polyhydroxyalkanoate (PHA) was also investigated. The optimal condition for bromelain partitioning was 15% polyethylene glycol (PEG)4000-20%K2HPO4. The highest activity recovery (80.14%) and enzyme purity was increased to 1.84-folds under optimal condition. The molecular weight of bromelain was 29 kDa. Bromelain yielded the highest activity when incubated with buffer pH 6.0 under 45°C. After purification and precipitation, the bromelain optimal pH changed from 6.0 to 7.0 and its optimum temperature was 50°C, however it kept activity even after 60°C. The activity of bromelain showed higher stability at pH 7.0-8.0 and was slightly stable at all pH levels tested. Its activity also decreased continuously with an increase of NaCl concentration. Partially purified bromelain was applied to recovery and purification of PHA from PHA-accumulating bacteria. The experiment was determined in 5 bacteria including both Gram-positive and Gram-negative strains. The result showed that bromelain was suitable for PHA purification. High PHA purity (82.7-96.5%) was observed in Gram-negative bacteria. The results were also compared with commercial bromelain. The lower efficiency was obtained from partially purified enzyme, but the content was not significantly different. 

Keywords: bromelain, enzyme partitioning, PHA, polyhydroxyalkanoate, pineapple stem

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Petikam Lavudu [a], Avula Prameela Rani [b], Chandra Bala Sekaran [c]*

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,032 Download: 327
Biochemical Characterization of Two Acid Phosphatases Purified from Breadfruit (Artocarpus communis) Seeds
page: 1100 - 1112

Hubert Kouassi Konan*, Desire Yapi Assoi Yapi, Clement Yao Yue Bi, Thierry Fankroma Martial Kone , Parfait Eugene Jean N’guessan Kouadio and Lucien Patrice Kouame

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 911 Download: 862
Protein Biomarker Screening on Effect of Freeze Dried Crocodile Blood and Vitamin C in Iron-deficient Anemic Rats
page: 1077 - 1088

Ekawit Threenet [a], Win Chaeychomsri [b], Sudawan Chaeychomsri [c] and Jindawan Siruntaweneti* [b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,439 Download: 429
Biofuel Production, Characterization and Degradation of 3-hydroxybutyate Methyl Ester from Polyhydroxybutyrate
page: 808 - 817

Kanokphorn Sangkharak*[a], Nisa Pichid [a], Tewan Yunu[a], Kamonthip Srinak [a], Sasithorn Sornnum [a] and Poonsuk Prasertsan [b]

Vol.43 No.4 (JULY 2016)
Research Article View: 974 Download: 374
Production of Talaromyces (Penicillium) marneffei Glyceraldehyde-3-Phosphate Dehydrogenase Recombinant Protein Expressed by Pichia pastoris
page: 470 - 476

Sophit Khanthawong* and Nongnuch Vanittanakom

Vol.43 No.3 (APRIL 2016)
Research Article View: 924 Download: 300
Sintered Dual-phase Steels Produced from Pre-alloyed Fe-Cr-Mo Powder
page: 358 - 364

Wananurat Srijampan [a], Monnapas Morakotjinda [b], Rungtip Krataitong [b], Thanyaporn Yotkaew [b], Nattaya Tosangthum [b], Amporn Wiengmoon [a] and Ruangdaj Tongsri *[b]

Vol.43 No.2 (SPECIAL ISSUE 1)
Research Article View: 949 Download: 403
Earthy-musty Odour and Off-flavour Taints in Phayao Lake, Thailand
page: 22 - 31

Supannee Suwanpakdee, Redel Gutierrez, Santiwat Pithakpol, Arunothai Jampeetong, Wasu Pathom-aree, Nakao Nomura, Tomoaki Itayama[f] and Niwooti Whangchai

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,012 Download: 398
Comparison of Some Modern IT Equipment as Detectors for Microfluidic Paper-based Determination of Phosphate
page: 176 - 182

Wasin Wongwilai [a], B. Manori Jayawardane [b], Spas D. Kolev [b], Kate Grudpan [a] and Ian D. McKelvie*[b,c]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 906 Download: 272
Assessment of genetic diversity of Jatropha curcas L. using AFLP and ISSR markers
page: 614 - 625

Sirisak Soonthornyatara, Prapa Sripichitt, Rugsarit Kaveeta and Vipa Hongtrakul

Vol.42 No.3 (JULY 2015)
Research Article View: 1,287 Download: 297
Phylogenetic Study of the Botryosphaeriaceae Species Associated with Avocado and Pará rubber in Thailand.
page: 104 - 116

Trakunyingcharoen T., Cheewangkoon R. and To-anun C.

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,564 Download: 296
Genetic Relationships among Indonesian Jatropha curcas L. Accessions, Selected Crossings, and Seed Oil Yield of Their Progenies
page: 1109 - 1120

Andi Wijayaa, b, Memen Surahmanc, S. Susantidianad, Benyamin Lakitana,e

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 922 Download: 299
Culturable Bacterial Community in a High Temperature EBPR Reactor
page: 970 - 980

Ying Hui Ong 1a, Adeline Seak May Chua 2a*, Geok Yuan Annie Tan3b, and Gek Cheng Ngoh 4a

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,035 Download: 383
Production of a rabbit polyclonal anti-elephant IgG antibody for the elephant laboratory investigation
page: 376 - 385

Preeyanat Vongchan*

Vol.40 No.3 (JULY 2013)
Research Article View: 912 Download: 323
Co-precipitation of Mefenamic acid and Polyethylene Glycol 4000 Using the Gas Anti-Solvent (GAS) Process
page: 440 - 446

Manop Charoenchaitrakool *[a, b], Siwaporn Suttikornchai [a, b] and Thitima Songjitsomboon [a]

Vol.40 No.3 (JULY 2013)
Research Article View: 910 Download: 392
Phytoplankton as a Bio-indicator of Water Quality in the Freshwater Fishing Area of Pak Phanang River Basin (Southern Thailand)
page: 344 - 355

Amphorn Sakset* [a] and Wanninee Chankaew* [b]

Vol.40 No.3 (JULY 2013)
Research Article View: 993 Download: 628
Micellar Liquid Chromatographic Determination of Sildenafil Citrate in Pharmaceutical Formulations
page: 408 - 418

Sujaritpun Jaipang [a], Dammrong Santiarworn [a], Saisunee Liawruangrath [b] and Boonsom Liawruangra

Vol.40 No.3 (JULY 2013)
Research Article View: 955 Download: 292
Effects of Calcination Conditions on Phase Formation and Particle Size of Zn2Nb34O87 Powders Synthesized by Solid-State Reaction
page: 135 - 142

Penphitcha Amonpattaratkit and Supon Ananta

Vol.40 No.1 (JANUARY 2013)
Research Article View: 796 Download: 288
Characteristics of Phosphate Solubilization by Phosphate-Solubilizing Bacteria Isolated from Agricultural Chili Soil and Their Efficiency on the Growth of Chili (Capsicum frutescens L. cv. Hua Rua)
page: 11 - 25

Worapon Surapat[a], Charida Pukahuta[a], Pongsak Rattanachaikunsopon[a], Tadanori Aimi[b], and Sopho

Vol.40 No.1 (JANUARY 2013)
Research Article View: 909 Download: 303
Post-treatment by Sodium Silicate after Zinc Phosphating on Cold-Rolled Steel
page: 30 - 40

Supunsa Dech-oup, Sirinee Thaiwatthana and Torranin Chairaungsri

Vol.39 No.1 (JANUARY 2012)
Research Article View: 934 Download: 307
An Orphan Crop (Cucumeropsis mannii Naudin) with Interesting Phosphatase, β-galactosidase and α-mannosidase Activities: Nutritional Approach and Biochemical Characterization
page: 210 - 222

Djary M. Koffi [a], Betty M. Faulet [b], Jean T. Gonnety [b], Mica l E. B dikou [a], Iri A. Zoro Bi

Vol.38 No.2 (APRIL 2011)
Research Article View: 923 Download: 263
Isothermal Phase Transformation Sequence in Fe-22wt%Cr-3.2wt%Mo-6.2wt%Ni-0.037wt%C Cast Duplex Stainless Steel
page: 47 - 55

Surasak Kuimalee*[a], John T.H. Pearce [b] and Torranin Chairuangsri**[c]

Vol.38 No.1 (JANUARY 2011)
Research Article View: 932 Download: 260
A Direct Current Polarographic Method for the Determination of Chlorpheniramine Maleate in Pharmaceutical Preparations
page: 135 - 142

Teraboon Pojanagaroon, Saisunee Liawruangrath, and Boonsom Liawruangrath

Vol.34 No.1 (JANUARY 2007)
Short Communication View: 916 Download: 629
Dirac Equation : Wave Function and Phase Shifts for a Linear Potential
page: 15 - 22

Lalit K. Sharma, and Lesolle D. Sebitla

Vol.34 No.1 (JANUARY 2007)
Research Article View: 827 Download: 326
Preliminary Study of Anthraquinone in Sweet Bamboo (Dendrocalamus asper Backer) Alkaline Sulfite Pulping
page: 235 - 247

Suphat Kamthai

Vol.34 No.2 (MAY 2007)
Research Article View: 1,559 Download: 1,551
Optimization Method for Determination of Carbofuran and Carboxin Residues in Cabbages by SPE and HPLC-UV
page: 227 - 234

Khin L. Zan, and Somporn Chantara

Vol.34 No.2 (MAY 2007)
Research Article View: 949 Download: 414
Finite Element Solution for 1-D Groundwater Flow, Advection-Dispersion, and Interphase Mass Transfer: II. Experimental Validation
page: 161 - 168

Satawat Saenton*[a] and Morrakot Khebchareon [b]

Vol.33 No.2 (MAY 2006)
Research Article View: 909 Download: 243
High Performance Thin Layer Chromatographic Method for the Determination of Diclofenac Sodium in Pharmaceutical Formulations
page: 123 - 128

Wisanu Thongchai, Boonsom Liawruangrath*, Chalermporn Thongpoon and Theeraphan Machan

Vol.33 No.1 (JANUARY 2006)
Research Article View: 1,586 Download: 397
Pressure-Temperature Estimates of the Metamorphosed Sedimentary Rocks on the Eastern Side of Khao Phai, Immediate West of Ban Choeng Chai, Wang Pong, Phetchabun Province, Thailand
page: 283 - 293

Yuenyong Panjasawatwong, Boontarika Srithai and Udomporn Wipakul

Vol.35 No.2 (MAY 2008)
Research Article View: 939 Download: 257
Novel Elastic Nanovesicles for Cosmeceutical and Pharmaceutical Applications
page: 168 - 178

Aranya Manosroi, Pensak Jantrawut, Narinthorn Khositsuntiwong, Worapaka Manosroi, and Jiradej Manosr

Vol.36 No.2 (MAY 2009)
Short Communication View: 1,007 Download: 400
Characterization of Perovskite Lead Iron Niobate Powders Synthesized by Partial Oxalate
page: 71 - 76

Pitak Youmee [a] and Sukon Phanichphant* [b]

Vol.32 No.1 (JANUARY 2005)
Short Communication View: 1,367 Download: 249
Stability Study of Six 1,4-Benzodiazepines in Bio-fluids Stored at -20oC
page: 451 - 463

Mohammad N. Uddin, Victoria F. Samanidou, and Ioannis N. Papadoyannis

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,056 Download: 525
Retention Behavior of Aromatic Amines with Some Ionic Liquids Mobile Phase in Semi-micro HPLC
page: 384 - 396

Yuppadee Nusai, Hitoshi Koizumi, Masaki Tachibana, Kazue Tani, and Nobutoshi Kiba

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,002 Download: 303
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