Retention Behavior of Aromatic Amines with Some Ionic Liquids Mobile Phase in Semi-micro HPLC
Yuppadee Nusai, Hitoshi Koizumi, Masaki Tachibana, Kazue Tani, and Nobutoshi Kiba* Author for corresponding; e-mail address: nusaijan@hotmail.com
Volume: Vol.37 No.3 (SEPTEMBER 2010)
Research Article
DOI:
Received: 5 January 2010, Revised: -, Accepted: 3 May 2010, Published: -
Citation: Nusai Y., Koizumi H., Tachibana M., Tani K. and Kiba N., Retention Behavior of Aromatic Amines with Some Ionic Liquids Mobile Phase in Semi-micro HPLC , Chiang Mai Journal of Science, 2010; 37(3): 384-396.
Abstract
This research was aimed at investigating the retention behavior of aromatic amines with some ionic liquid mobile phases in semi-micro HPLC to explain the unclear separation mechanism. Aromatic amines (m-aminophenol, benzylamine, N,N-dimethylaniline, p-aminobenzoic acid, p-aminophenol, aniline, p-toluidine and N-methylaniline) were examined. The retention behavior of aromatic amines is greatly affected by the column equilibration time, ionic liquid concentration, mobile phase pH and alkyl chain length on the imidazolium cation of the ionic liquid. Semi-micro HPLC was used to increase separation power and sensitivity, and in addition to save on the mobile phase of the expensive ionic liquid. The separation mechanism involves ionic and hydrophobic interactions.