Production and Immobilization of Levansucrase

Surawut Sangmanee, Santhana Nakapong, Kamontip Kuttiyawong and Rath Pichyangkura
* Author for corresponding; e-mail address: prath@chula.ac.th
Volume: Vol.42 No.1 (JANUARY 2015)
Research Article
DOI:
Received: 24 December 2013, Revised: -, Accepted: 5 May 2014, Published: -

Citation: Sangmanee S., Nakapong S., Kuttiyawong K. and Pichyangkura R., Production and Immobilization of Levansucrase , Chiang Mai Journal of Science, 2015; 42(1): 44-51.

Abstract

Escherichia coli Top-10 containing a levansucrase gene (lsRN) of Bacillus licheniformis RN-01, cultivated in 3X LB medium, produced levansucrase at 65.7 U/ml of culture medium. The purified levansucrase had a MW of 52 kDa and specific activity of 170.04 U/mg protein with 6.6 purification fold and 62.2% yield. B. licheniformis RN-01 levansucrase was covalently bound on chitosan beads, Sepabead EC-EP beads, and Sepabead EC-HFA beads with the immobilization efficiency of 96%, 35%, and 23%, respectively. Levansucrase immobilized on chitosan beads retained over 75% of its activity after 10 cycles of repetitive use. In contrast, levansucrase immobilized on Sepabead EC-EP or Sepabead EC-HFA lost over 60% after only 5 cycles of repetitive used. The optimum pH and temperature of the immobilized enzyme on chitosan beads (pH 4.0-6.0, 40-50^oC) were significantly broader than those of the free enzyme (pH 6.0, 50^oC). These results demonstrated that chitosan beads have superb characteristics for levansucrase immobilization.

Keywords: L-FOS, fructooligosaccharide, levansucrase, chitosan, Bacillus licheniformis, Escherichia coli Top-10

Enhancement of Antioxidant Activity and Inhibition of Polyphenol Oxidase from Green Oak Lettuce by Chitosan-Encapsulated Rambutan (Nephelium lappaceum L.) Peel Extract
DOI: 10.12982/CMJS.2025.035.

Panita Kongsune, Kittisak Sangthongchin, Phasin Ampha, Manee Maneechote and Netnapa Chana

Vol.52 No.4 (July 2025)
Research Article View: 1,555 Download: 528

Optimization of Chitosan-Alginate Hydrogel Properties for Controlled Release of Urea
DOI: 10.12982/CMJS.2025.008.

Melody Kimi and Chee Jiun Chong

Vol.52 No.1 (January 2025)
Research Article View: 894 Download: 1,099

Biosurfactant-Producing Bacteria and Microbial Analysis in Mae Soon Reservoir, Fang Oil Field, Chiang Mai, Thailand
DOI: 10.12982/CMJS.2023.024.

Ladapa Kumdhitiahutsawakul, Thanachai Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Yingmanee Tragoolpua and Sakunnee Bovonsombut

Vol.50 No.3 (May 2023)
Research Article View: 1,851 Download: 609

Bacterial Cellulose and Bacterial Cellulose/Chitosan Films Containing Mangosteen Pericarp Extract for Wound Dressings
page: 952 - 968

Pronpatsorn Moonsungnoen, Duangjai Ochaikul and Pathavuth Monvisade

Vol.48 No.4 (July 2021)
Research Article View: 1,830 Download: 688

Optimal Conditions for Lignin Coagulation in Lignin Contaminated Water
page: 123 - 131

Surin Saipanya*, Ruangsri Watanesk, Wimol Saiyasombat and Sunanta Wangkarn

Vol.30 No.2 (AUGUST 2003)
Research Article View: 953 Download: 329

Pencil Graphite Based Biosensor for Rapid Malathion Pesticide Detection
page: 2374 - 2380

Lerdluck Kaewvimol

Vol.45 No.6 (September 2018)
Research Article View: 1,015 Download: 378

Applications of Oxidized Chitosan for Scale Prevention in Sugar Evaporators
page: 961 - 972

Tu Phuong Pham Le and Wanwipa Siriwatwechakul*

Vol.45 No.2 (March 2018)
Research Article View: 977 Download: 370

Scaffolds of Fish Skin Gelatin from Unicorn Leatherjackets (Aluterus monoceros) Reinforced with Chitosan for Bone Tissue Engineering
page: 1611 - 1623

Safiah Saah, Kwunchit Oungbho* and Soottawat Benjakul

Vol.44 No.4 (October 2017)
Research Article View: 1,024 Download: 402

Antibacterial Activity Against Acne Involved Bacteria of Chitosan in a Soluble State and as Nanoparticles
page: 1149 - 1158

Supreedee Sungkharak [a, b], Nicha Supasit [b], Sasiluk Choopan [b] and Suwipa Ungphaiboon*[a, b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,100 Download: 773

Effects of Chitosan and Salicylic Acid on Stemona Alkaloid Production in Hydroponic Culture of Stemona curtisii Hook. f.
page: 1070 - 1076

Jiraporn Palee [a], Srisulak Dheeranupattana* [b], Sunanta Wangkarn [c], Stephen G. Pyne [d] and Alison T. Ung [e]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 927 Download: 343

Encapsulation of Artocarpus lacucha Roxb. Extract in Alginate Chitosan Nanoparticles for Inhibition of Methicillin Resistant Staphylococcus aureus and Bacteria Causing Skin Diseases
page: 946 - 958

Thida Kaewkod [a], Khajornsak Tragoolpua [b] and Yingmanee Tragoolpua* [a]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 942 Download: 397

Biofuel Production, Characterization and Degradation of 3-hydroxybutyate Methyl Ester from Polyhydroxybutyrate
page: 808 - 817

Kanokphorn Sangkharak*[a], Nisa Pichid [a], Tewan Yunu[a], Kamonthip Srinak [a], Sasithorn Sornnum [a] and Poonsuk Prasertsan [b]

Vol.43 No.4 (JULY 2016)
Research Article View: 950 Download: 374

Analysis of Chitin, Chitosan, and Optimization for Carotenoids Extraction Yield with Rice Bran Oil from Thai Fairy Shrimp
page: 918 - 929

Tanyarat Poothawan and Khomsorn Lomthaisong

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 999 Download: 408

Emulsions stabilized by chitosan/lithium dodecyl sulfate complexes
page: 393 - 400

Thammasit Vongsetskul, Kewalin Phaenthong, Ratana Chanthateyanonth, Panya Sunintaboon and Pramuan T

Vol.42 No.2 (APRIL 2015)
Research Article View: 11,323 Download: 482

Effect of Relative Humidity on MechanicalProperties of Blended Chitosan-Methylcellulose Film
page: 133 - 137

Pornchai Rachtanapun and Panchat Wongchaiya

Vol.39 No.1 (JANUARY 2012)
Short Communication View: 944 Download: 197

Effect of Chitosan and Mordants on Dyeability of Cotton Fabrics with Ruellia tuberosa Linn.
page: 95 - 104

Piyaporn Kampeerapappun, Trongsu Phattararittigul, Sutida Jittrong and Dararat Kullachod

Vol.38 No.1 (JANUARY 2011)
Research Article View: 1,024 Download: 284

Comparison of Sludge Granule and UASB Performance by Adding Chitosan in Different Forms
page: 95 - 102

Boonyarit Nuntakumjorn, Wuthichok Khumsalud, Naret Vetsavas, Thoranin Sujjaviriyasup and Chantarapor

Vol.35 No.1 (JANUARY 2008)
Research Article View: 941 Download: 360

Artificial Tear Formulation from Chitosan
page: 501 - 505

Phuriwat L eesawat *[a], K arn V earnsilp[a], Nijaporn Y anasarn [a] and Peeracha Thanawatta

Vol.32 No.3 (SEPTEMBER 2005)
Research Article View: 915 Download: 363

Chiang Mai Journal of Science

1,647

Q3 0.80

Q3 1.3

7 days

Outline

Figures and Tables