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Production and Immobilization of Levansucrase
Paper Type  |
Contributed Paper |
Title |
Production and Immobilization of Levansucrase |
Author |
Surawut Sangmanee, Santhana Nakapong, Kamontip Kuttiyawong and Rath Pichyangkura |
Email |
prath@chula.ac.th |
|
Abstract: Escherichia coli Top-10 containing a levansucrase gene (lsRN) of Bacillus licheniformis RN-01, cultivated in 3X LB medium, produced levansucrase at 65.7 U/ml of culture medium. The purified levansucrase had a MW of 52 kDa and specific activity of 170.04 U/mg protein with 6.6 purification fold and 62.2% yield. B. licheniformis RN-01 levansucrase was covalently bound on chitosan beads, Sepabead EC-EP beads, and Sepabead EC-HFA beads with the immobilization efficiency of 96%, 35%, and 23%, respectively. Levansucrase immobilized on chitosan beads retained over 75% of its activity after 10 cycles of repetitive use. In contrast, levansucrase immobilized on Sepabead EC-EP or Sepabead EC-HFA lost over 60% after only 5 cycles of repetitive used. The optimum pH and temperature of the immobilized enzyme on chitosan beads (pH 4.0-6.0, 40-50oC) were significantly broader than those of the free enzyme (pH 6.0, 50oC). These results demonstrated that chitosan beads have superb characteristics for levansucrase immobilization. |
|
Start & End Page |
44 - 51 |
Received Date |
2013-12-24 |
Revised Date |
|
Accepted Date |
2014-05-05 |
Full Text |
Download |
Keyword |
L-FOS, fructooligosaccharide, levansucrase, chitosan, Bacillus licheniformis, Escherichia coli Top-10 |
Volume |
Vol.42 No.1 (JANUARY 2015) |
DOI |
|
Citation |
Sangmanee S., Nakapong S., Kuttiyawong K. and Pichyangkura R., Production and Immobilization of Levansucrase , Chiang Mai Journal of Science, 2015; 42(1): 44-51. |
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