Submit your paper 
Download Template JOURNAL DETAIL
Production and Immobilization of Levansucrase
Paper Type  |
Contributed Paper |
Title |
Production and Immobilization of Levansucrase |
Author |
Surawut Sangmanee, Santhana Nakapong, Kamontip Kuttiyawong and Rath Pichyangkura |
Email |
prath@chula.ac.th |
|
Abstract: Escherichia coli Top-10 containing a levansucrase gene (lsRN) of Bacillus licheniformis RN-01, cultivated in 3X LB medium, produced levansucrase at 65.7 U/ml of culture medium. The purified levansucrase had a MW of 52 kDa and specific activity of 170.04 U/mg protein with 6.6 purification fold and 62.2% yield. B. licheniformis RN-01 levansucrase was covalently bound on chitosan beads, Sepabead EC-EP beads, and Sepabead EC-HFA beads with the immobilization efficiency of 96%, 35%, and 23%, respectively. Levansucrase immobilized on chitosan beads retained over 75% of its activity after 10 cycles of repetitive use. In contrast, levansucrase immobilized on Sepabead EC-EP or Sepabead EC-HFA lost over 60% after only 5 cycles of repetitive used. The optimum pH and temperature of the immobilized enzyme on chitosan beads (pH 4.0-6.0, 40-50oC) were significantly broader than those of the free enzyme (pH 6.0, 50oC). These results demonstrated that chitosan beads have superb characteristics for levansucrase immobilization. |
|
Start & End Page |
44 - 51 |
Received Date |
2013-12-24 |
Revised Date |
|
Accepted Date |
2014-05-05 |
Full Text |
Download |
Keyword |
L-FOS, fructooligosaccharide, levansucrase, chitosan, Bacillus licheniformis, Escherichia coli Top-10 |
Volume |
Vol.42 No.1 (JANUARY 2015) |
DOI |
|
Citation |
Sangmanee S., Nakapong S., Kuttiyawong K. and Pichyangkura R., Production and Immobilization of Levansucrase , Chiang Mai Journal of Science, 2015; 42(1): 44-51. |
SDGs |
|
| View:687 Download:211 | |
RELATED ARTICLE
Optimization of Chitosan-Alginate Hydrogel Properties for Controlled Release of Urea
Article ID: e2025008
Author:Melody Kimi and Chee Jiun Chong
Vol.52 No.1 (January 2025) View: 246 Download:107
Article ID: e2025008
Author:Melody Kimi and Chee Jiun Chong
Vol.52 No.1 (January 2025) View: 246 Download:107
Biosurfactant-Producing Bacteria and Microbial Analysis in Mae Soon Reservoir, Fang Oil Field, Chiang Mai, Thailand
Article ID: e2023024
Author:Ladapa Kumdhitiahutsawakul, Thanachai Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Yingmanee Tragoolpua and Sakunnee Bovonsombut
Vol.50 No.3 (May 2023) View: 676 Download:419
Article ID: e2023024
Author:Ladapa Kumdhitiahutsawakul, Thanachai Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Yingmanee Tragoolpua and Sakunnee Bovonsombut
Vol.50 No.3 (May 2023) View: 676 Download:419
SDGs:
Bacterial Cellulose and Bacterial Cellulose/Chitosan Films Containing Mangosteen Pericarp Extract for Wound Dressings
page: 952 - 968
Author:Pronpatsorn Moonsungnoen, Duangjai Ochaikul and Pathavuth Monvisade
Vol.48 No.4 (July 2021) View: 1,449 Download:605
page: 952 - 968
Author:Pronpatsorn Moonsungnoen, Duangjai Ochaikul and Pathavuth Monvisade
Vol.48 No.4 (July 2021) View: 1,449 Download:605
Optimal Conditions for Lignin Coagulation in Lignin Contaminated Water
page: 123 - 131
Author:Surin Saipanya*, Ruangsri Watanesk, Wimol Saiyasombat and Sunanta Wangkarn
Vol.30 No.2 (AUGUST 2003) View: 660 Download:257
page: 123 - 131
Author:Surin Saipanya*, Ruangsri Watanesk, Wimol Saiyasombat and Sunanta Wangkarn
Vol.30 No.2 (AUGUST 2003) View: 660 Download:257
Pencil Graphite Based Biosensor for Rapid Malathion Pesticide Detection
page: 2374 - 2380
Author:Lerdluck Kaewvimol
Vol.45 No.6 (September 2018) View: 780 Download:266
page: 2374 - 2380
Author:Lerdluck Kaewvimol
Vol.45 No.6 (September 2018) View: 780 Download:266
Applications of Oxidized Chitosan for Scale Prevention in Sugar Evaporators
page: 961 - 972
Author:Tu Phuong Pham Le and Wanwipa Siriwatwechakul*
Vol.45 No.2 (March 2018) View: 700 Download:301
page: 961 - 972
Author:Tu Phuong Pham Le and Wanwipa Siriwatwechakul*
Vol.45 No.2 (March 2018) View: 700 Download:301
Scaffolds of Fish Skin Gelatin from Unicorn Leatherjackets (Aluterus monoceros) Reinforced with Chitosan for Bone Tissue Engineering
page: 1611 - 1623
Author:Safiah Saah, Kwunchit Oungbho* and Soottawat Benjakul
Vol.44 No.4 (October 2017) View: 786 Download:305
page: 1611 - 1623
Author:Safiah Saah, Kwunchit Oungbho* and Soottawat Benjakul
Vol.44 No.4 (October 2017) View: 786 Download:305
Antibacterial Activity Against Acne Involved Bacteria of Chitosan in a Soluble State and as Nanoparticles
page: 1149 - 1158
Author:Supreedee Sungkharak [a, b], Nicha Supasit [b], Sasiluk Choopan [b] and Suwipa Ungphaiboon*[a, b]
Vol.43 No.5 (OCTOBER 2016) View: 726 Download:527
page: 1149 - 1158
Author:Supreedee Sungkharak [a, b], Nicha Supasit [b], Sasiluk Choopan [b] and Suwipa Ungphaiboon*[a, b]
Vol.43 No.5 (OCTOBER 2016) View: 726 Download:527
Effects of Chitosan and Salicylic Acid on Stemona Alkaloid Production in Hydroponic Culture of Stemona curtisii Hook. f.
page: 1070 - 1076
Author:Jiraporn Palee [a], Srisulak Dheeranupattana* [b], Sunanta Wangkarn [c], Stephen G. Pyne [d] and Alison T. Ung [e]
Vol.43 No.5 (OCTOBER 2016) View: 644 Download:273
page: 1070 - 1076
Author:Jiraporn Palee [a], Srisulak Dheeranupattana* [b], Sunanta Wangkarn [c], Stephen G. Pyne [d] and Alison T. Ung [e]
Vol.43 No.5 (OCTOBER 2016) View: 644 Download:273
Encapsulation of Artocarpus lacucha Roxb. Extract in Alginate Chitosan Nanoparticles for Inhibition of Methicillin Resistant Staphylococcus aureus and Bacteria Causing Skin Diseases
page: 946 - 958
Author:Thida Kaewkod [a], Khajornsak Tragoolpua [b] and Yingmanee Tragoolpua* [a]
Vol.43 No.5 (OCTOBER 2016) View: 681 Download:318
page: 946 - 958
Author:Thida Kaewkod [a], Khajornsak Tragoolpua [b] and Yingmanee Tragoolpua* [a]
Vol.43 No.5 (OCTOBER 2016) View: 681 Download:318
Biofuel Production, Characterization and Degradation of 3-hydroxybutyate Methyl Ester
from Polyhydroxybutyrate
page: 808 - 817
Author:Kanokphorn Sangkharak*[a], Nisa Pichid [a], Tewan Yunu[a], Kamonthip Srinak [a], Sasithorn Sornnum [a] and Poonsuk Prasertsan [b]
Vol.43 No.4 (JULY 2016) View: 678 Download:315
page: 808 - 817
Author:Kanokphorn Sangkharak*[a], Nisa Pichid [a], Tewan Yunu[a], Kamonthip Srinak [a], Sasithorn Sornnum [a] and Poonsuk Prasertsan [b]
Vol.43 No.4 (JULY 2016) View: 678 Download:315
Analysis of Chitin, Chitosan, and Optimization for Carotenoids Extraction Yield with Rice Bran Oil from Thai Fairy Shrimp
page: 918 - 929
Author:Tanyarat Poothawan and Khomsorn Lomthaisong
Vol.42 No.4 (OCTOBER 2015) View: 667 Download:215
page: 918 - 929
Author:Tanyarat Poothawan and Khomsorn Lomthaisong
Vol.42 No.4 (OCTOBER 2015) View: 667 Download:215
Emulsions stabilized by chitosan/lithium dodecyl sulfate complexes
page: 393 - 400
Author:Thammasit Vongsetskul, Kewalin Phaenthong, Ratana Chanthateyanonth, Panya Sunintaboon and Pramuan T
Vol.42 No.2 (APRIL 2015) View: 10,760 Download:321
page: 393 - 400
Author:Thammasit Vongsetskul, Kewalin Phaenthong, Ratana Chanthateyanonth, Panya Sunintaboon and Pramuan T
Vol.42 No.2 (APRIL 2015) View: 10,760 Download:321
Effect of Relative Humidity on MechanicalProperties of Blended Chitosan-Methylcellulose Film
page: 133 - 137
Author:Pornchai Rachtanapun and Panchat Wongchaiya
Vol.39 No.1 (JANUARY 2012) View: 700 Download:143
page: 133 - 137
Author:Pornchai Rachtanapun and Panchat Wongchaiya
Vol.39 No.1 (JANUARY 2012) View: 700 Download:143
Effect of Chitosan and Mordants on Dyeability of Cotton Fabrics with Ruellia tuberosa Linn.
page: 95 - 104
Author:Piyaporn Kampeerapappun, Trongsu Phattararittigul, Sutida Jittrong and Dararat Kullachod
Vol.38 No.1 (JANUARY 2011) View: 713 Download:219
page: 95 - 104
Author:Piyaporn Kampeerapappun, Trongsu Phattararittigul, Sutida Jittrong and Dararat Kullachod
Vol.38 No.1 (JANUARY 2011) View: 713 Download:219
Comparison of Sludge Granule and UASB
Performance by Adding Chitosan in Different Forms
page: 95 - 102
Author:Boonyarit Nuntakumjorn, Wuthichok Khumsalud, Naret Vetsavas, Thoranin Sujjaviriyasup and Chantarapor
Vol.35 No.1 (JANUARY 2008) View: 665 Download:274
page: 95 - 102
Author:Boonyarit Nuntakumjorn, Wuthichok Khumsalud, Naret Vetsavas, Thoranin Sujjaviriyasup and Chantarapor
Vol.35 No.1 (JANUARY 2008) View: 665 Download:274
Artificial Tear Formulation from Chitosan
page: 501 - 505
Author:Phuriwat L eesawat *[a], K arn V earnsilp[a], Nijaporn Y anasarn [a] and Peeracha Thanawatta
Vol.32 No.3 (SEPTEMBER 2005) View: 648 Download:274
page: 501 - 505
Author:Phuriwat L eesawat *[a], K arn V earnsilp[a], Nijaporn Y anasarn [a] and Peeracha Thanawatta
Vol.32 No.3 (SEPTEMBER 2005) View: 648 Download:274
Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science