Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Optimization of Chitosan-Alginate Hydrogel Properties for Controlled Release of Urea

Melody Kimi and Chee Jiun Chong
* Author for corresponding; e-mail address: kmelody@unimas.my
Volume: Vol.52 No.1 (January 2025)
Research Article
DOI: https://doi.org/10.12982/CMJS.2025.008
Received: 18 November 2024, Revised: 31 December 2024, Accepted: 2 January 2025, Published: 29 January 2025

Citation: Kimi M. and Chong C.J., Optimization of chitosan-alginate hydrogel properties for controlled release of urea. Chiang Mai Journal of Science, 2025; 52(1): e2025008. DOI 10.12982/CMJS.2025.008.

Graphical Abstract

Graphical Abstract

Abstract

     This study explored the effects of synthesis parameters such as chitosan molecular weight, alginate loading, and crosslinker loading in optimizing chitosan-alginate hydrogel using response surface methodology prepared by ionotropic gelation. Box-Behnken design evaluated the effects of chitosan molecular weight, alginate loading (2.5 to 4.5 wt%), and crosslinker loading (0.10 M to 1.00 M) on encapsulation efficiency, swelling behaviour and urea release. The hydrogels were spherical and with a mean diameter of 3.82 ± 0.78 μm. FTIR analysis confirmed the interactions between chitosan, alginate and crosslinker added. Encapsulation efficiency was maximized at 89.43 ± 12.66%, with alginate concentration (3.5 wt%) being the most significant factor (p = 0.001264). Higher alginate concentrations improved encapsulation by facilitating crosslinking with Ca²⁺ ions. Swelling studies revealed an average swelling capacity of 33.27 ± 17.26%, peaking at 77%. Urea release over 2 hours averaged 20.08 ± 16.39%, with minimal release observed in formulations using lower molecular weight chitosan and moderate alginate concentrations (p = 0.01535). Statistical models indicated high adequacy for encapsulation efficiency (R² = 0.844) but limited predictive power for swelling (R² = 0.4987). The findings demonstrated the influence of formulation variables on hydrogel properties, underscoring their potential as nutrient delivery systems with controlled release and swelling behaviour.

Keywords: chitosan, alginate, urea, hydrogel, controlled release

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