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Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University
 


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Rice Bran Protein-Based Microbeads As Entrapping Carriers To Improve Stability of Enterococcus faecalis HZNU S1


Paper Type 
Contributed Paper
Title 
Rice Bran Protein-Based Microbeads As Entrapping Carriers To Improve Stability of Enterococcus faecalis HZNU S1
Author 
Zhen-Xing Tang, Qiu-Yu Wang, Hai Zhou, Yan-Yu Hu, Xing-Pei Ye, Zhong-Bao Jiang, Hui-Yang Shen and Lu-E Shi
Email 
shilue@126.com, tangzhenxing@126.com
Abstract:

     The primary purpose of the current research was to study the influence of entrapment on E. faecalis HZNU S1 under simulated gastrointestinal and different storage conditions. To achieve this, the cell was entrapped by sodium alginate (ALG) and rice bran protein (RBP) as wall materials. Probiotic survival under storage and simulated gastrointestinal conditions were explored, as well as entrapment yield (EY) and the size of microbeads. EY was around 98.5 %, while the diameter was 1.75 ± 0.15 mm. In simulated gastrointestinal tests, entrapment provided significant protection against viability loss compared to the control. Entrapped cells could be completely released within 2 h in simulated intestinal juice (SIJ) approximately. In the storage stability tests, entrapment also promoted greater protection for probiotics in relation to the free cells. The viable cells of entrapped cells retained more than 7.0 Log CFU/g after one month of storage (4 °C), and higher than 6.0 Log CFU/g at 25 °C when being stored for 8 days, respectively. Entrapped E. faecalis HZNU S1 exhibited increased tolerance to external conditions, making it a suitable candidate for delivering probiotics to enhance the related product value.

Article ID
e2025004
Received Date 
2024-08-28
Revised Date 
2024-12-10
Accepted Date 
2024-12-13
Published Date 
2025-01-29
Full Text 
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Keyword 
Enterococcus faecalis, encapsulating, rice bran protein, alginate, storage, viability
Volume 
Vol.52 No.1 (January 2025)
DOI 
https://doi.org/10.12982/CMJS.2025.004
Citation 
Tang Z.,Wang Q.,Zhou H.,Hu Y.,Ye X.,Jiang Z., et al., Rice Bran Protein-Based Microbeads As Entrapping Carriers To Improve Stability of Enterococcus faecalis HZNU S1, Chiang Mai Journal of Science, 2025; 52(1): e2025004. DOI 10.12982/CMJS.2025.004.
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