Chiang Mai Journal of Science

     The primary purpose of the current research was to study the influence of entrapment on E. faecalis HZNU S1 under simulated gastrointestinal and different storage conditions. To achieve this, the cell was entrapped by sodium alginate (ALG) and rice bran protein (RBP) as wall materials. Probiotic survival under storage and simulated gastrointestinal conditions were explored, as well as entrapment yield (EY) and the size of microbeads. EY was around 98.5 %, while the diameter was 1.75 ± 0.15 mm. In simulated gastrointestinal tests, entrapment provided significant protection against viability loss compared to the control. Entrapped cells could be completely released within 2 h in simulated intestinal juice (SIJ) approximately. In the storage stability tests, entrapment also promoted greater protection for probiotics in relation to the free cells. The viable cells of entrapped cells retained more than 7.0 Log CFU/g after one month of storage (4 °C), and higher than 6.0 Log CFU/g at 25 °C when being stored for 8 days, respectively. Entrapped E. faecalis HZNU S1 exhibited increased tolerance to external conditions, making it a suitable candidate for delivering probiotics to enhance the related product value.