High Subzero Temperature Preservation of Spirulina platensis (Atrhrospira fusiformis) and Its Ultrastucture
Manita Motham, Yuwadee Peerapornpisal, Sudaporn Tongsriri, Chayakorn Pumas and Panmuk Vacharapiyasop* Author for corresponding; e-mail address: m_motham@yahoo.com
Volume: Vol.39 No.4 (OCTOBER 2012)
Research Article
DOI:
Received: 2 November 2011, Revised: -, Accepted: 2 July 2012, Published: -
Citation: Motham M., Peerapornpisal Y., Tongsriri S., Pumas C. and Vacharapiyasop P., High Subzero Temperature Preservation of Spirulina platensis (Atrhrospira fusiformis) and Its Ultrastucture, Chiang Mai Journal of Science, 2012; 39(4): 554-561.
Abstract
The high subzero preservation of Spirulina: S. platensis CMU2 and S. platensis GD1 was performed by step wise freezing at 25, 4, and -20°C for 30 min. Then they were preserved at -80°C for seven months with four cryoprotectats: dimethyl sulfoxide, horse serum, calf serum and glycerol. The viability of these two strains and cell concentration was not significantly different (p > 0.05). The survival of S.platensis depended on type and concentration of cryoprotectant and thawing temperature. The best condition for high subzero preservation was obtained by using 5% glycerol as cryoprotectant and rapid thawing at 40°C. After high subzero preservation, their ultrastructure such as cell wall, thylakoid and nucleoplasmic region were changed but cryoprotectant helped to protect these important organelles from freeze induced damage.