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Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University
 


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Scaffolds of Fish Skin Gelatin from Unicorn Leatherjackets (Aluterus monoceros) Reinforced with Chitosan for Bone Tissue Engineering


Paper Type 
Contributed Paper
Title 
Scaffolds of Fish Skin Gelatin from Unicorn Leatherjackets (Aluterus monoceros) Reinforced with Chitosan for Bone Tissue Engineering
Author 
Safiah Saah, Kwunchit Oungbho* and Soottawat Benjakul
Email 
k.oungbho@gmail.com
Abstract:
The aim of this study was to fabricate and characterize scaffolds of fish skin gelatin from unicorn leatherjackets (Aluterus monoceros) reinforced with chitosan for application in bone tissue engineering. The fish gelatin was extracted at 17% yield on the basis of the fish skin, and characterized according to AOAC official methods of analysis. As the fish gelatin exhibited low gel strength, fabrication of chitosan/fish gelatin (CG) scaffolds crosslinked with glutaraldehyde was performed by freeze-drying technique. The superior porous structure, mechanical, and swelling properties of the CG scaffolds in comparison to those of the single component scaffolds were observed. The scaffolds were nontoxic to MC3T3-E1 cells on MTT assay, supported biomineralization, and promoted cell attachment. It can be therefore concluded that the extracted fish gelatin is a feasible biomaterial for scaffold fabrication by reinforcement with chitosan.  
Start & End Page 
1611 - 1623
Received Date 
2015-09-03
Revised Date 
Accepted Date 
2016-02-09
Full Text 
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Keyword 
Unicorn leatherjacket, Fish gelatin, Chitosan, Scaffold, Bone tissue engineering
Volume 
Vol.44 No.4 (October 2017)
DOI 
Citation 
Saah S., Oungbho* K. and Benjakul S., Scaffolds of Fish Skin Gelatin from Unicorn Leatherjackets (Aluterus monoceros) Reinforced with Chitosan for Bone Tissue Engineering, Chiang Mai Journal of Science, 2017; 44(4): 1611-1623.
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