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Pencil Graphite Based Biosensor for Rapid Malathion Pesticide Detection
Paper Type  |
Contributed Paper |
Title |
Pencil Graphite Based Biosensor for Rapid Malathion Pesticide Detection |
Author |
Lerdluck Kaewvimol |
Email |
lerdluck.k@mail.rmutk.ac.th |
|
Abstract: This research described a sensitive deposable electrochemical biosensor for the detection of organophosphate pesticide based on acetylcholinesterase enzyme (AChE). The pencil graphite electrode was applied as working electrode to modify a disposable biosensor. A 0.5 U mg-1 AChE and 0.25 %wt Chitosan (Chit) were modified as enzyme and conductive polymer to produce PGE/PB/AChE/Chit biosensor by dropping method. Malathion was applied as a pesticide sample in this research. The detection conditions were employed under potential 0.3 V in 0.1 M phosphate buffer solution (pH 7.0) containing 15 mM acetylcholine chloride by chronoamperometry (CA). The PGE/PB/AChE/Chit biosensor exhibited detection limits at 3.0607 ng ml-1. The linear range, sensitivity, response time, reproducibility are 0.5-16.5 ngml-1, 0.8867 mA nM-1, 50 s, 4.43% RSD, respectively. The storage performance at 35 days were 77.97% remained. The proposed biosensor was successfully applied in the determination of malathion pesticide sample. |
|
Start & End Page |
2374 - 2380 |
Received Date |
2017-12-06 |
Revised Date |
|
Accepted Date |
2018-04-09 |
Full Text |
Download |
Keyword |
enzyme biosensor, Chitosan, AChE, malathion, pencil graphite electrode |
Volume |
Vol.45 No.6 (September 2018) |
DOI |
|
Citation |
Kaewvimol L., Pencil Graphite Based Biosensor for Rapid Malathion Pesticide Detection , Chiang Mai Journal of Science, 2018; 45(6): 2374-2380. |
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