Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

1,647
Articles
Q3 0.80
Impact Factor
Q3 1.3
CiteScore
7 days
Avg. First Decision
Outline
Figures and Tables
ARTICLE METRICS
Views 1,306
Downloads 552

Multi Chloroplast Genes for Species Identification in Bar-HRM Analysis of Taxonomical Complex Medicinal Plants Group (Zingiberaceae)

Sarawut Ounjai, Rossarin Osathanunkul, Panagiotis Madesis and Maslin Osathanunkul
* Author for corresponding; e-mail address: omaslin@gmail.com
Volume: Vol.44 No.4 (October 2017)
Research Article
DOI:
Received: 6 January 2016, Revised: -, Accepted: 23 June 2016, Published: -

Citation: Ounjai S., Osathanunkul R., Madesis P. and Osathanunkul M., Multi Chloroplast Genes for Species Identification in Bar-HRM Analysis of Taxonomical Complex Medicinal Plants Group (Zingiberaceae), Chiang Mai Journal of Science, 2017; 44(4): 1311-1321.

Abstract

The economic importance of the herbal industry is increasing every year. As the herbal industry grows, consumer safety is one issue that cannot be overlooked. Buying herbal products poses a risk of acquiring counterfeited and adulterated products. Due to these issues, a reliable method to authenticate products is needed. Here DNA barcoding coupled with High Resolution Melting analysis (Bar-HRM), an emerging method for species identification, was developed. The four plastid barcodes were selected to test its efficiency in identifying the three closely related Zingiberaceae species. None of the primers derived from single locus can be discriminate all tested species whilst combination of two primer pairs proved to be more effective at discrimination. However, as DNA could be extracted from dried samples, the Bar-HRM here poses a great potential in applying for authentication of the tested Zingiberaceae herbal products in processed forms.

Keywords: Zingiberaceae, DNA barcoding, HRM, identification, medicinal plant

Related Articles

A New Approach for Machine Learning-Based Recognition of Meat Species Using a BME688 Gas Sensors Matrix
DOI: 10.12982/CMJS.2025.031.

Nursel Söylemez Milli, İsmail Hakkı Parlak and Mehmet Milli

Vol.52 No.3 (May 2025)
Research Article View: 890 Download: 826
Revised and Updated Checklist of Macrofungi in Thailand – II
DOI: 10.12982/CMJS.2025.027.

Nakarin Suwannarach, Jaturong Kumla, Wenhua Lu, Atsadawut Kaewnunta, Naritsada Thongklang, Phongeun Sysouphanthong, Yuan-Shuai Liu, Thatsanee Luangharn, Nopparat Wannathes, Arttapon Walker, Nattapol Kraisitudomsook, Pumin Nutaratat, Sarunyou Wongkanoun, Esteban Charria-Girón, Samantha C. Karunarathna, Wasu Pathom-aree and Saisamorn Lumyong

Vol.52 No.3 (May 2025)
Review Article View: 1,204 Download: 322
Research Advances in Taxonomy, Phylogeny, Ecology, and Distribution of the Genus Cladophialophora
DOI: 10.12982/CMJS.2024.004.

Tanapol Thitla, Jaturong Kumla, Surapong Khuna, Sirasit Srinuanpan, Sinang Hongsanan, Chanokned Senwanna, Saowaluck Tibpromma, Wasu Pathom-aree, Saisamorn Lumyong and Nakarin Suwannarach

Vol.51 No.1 (January 2024)
Review Article View: 1,899 Download: 875
First Report of Curvularia hominis as a Causal Agent of Riceberry Rice Leaf Spot in Northern Thailand
DOI: 10.12982/CMJS.2023.066.

Dorkrak Chaisarn, Nakarin Suwannarach, Saisamorn Lumyong , Somchit Youpensuk and Wasu Pathom-aree

Vol.50 No.6 (November 2023)
Short Communication View: 990 Download: 935
Global Challenges in Identifying Plant Pathogenic Fungi: An Overview with Suggestions for the Way Forward
DOI: 10.12982/CMJS.2023.039.

Monika C. Dayarathne, Kasun M. Thambugala, Dinushani A. Daranagama, Amin U. Mridha, Asha J. Dissanayake, Lakmali S. Dissanayake, Sajeewa S.N. Maharachchikumbura, RMCS Rathnayake, Saisamorn Lumyong and Sinang Hongsanan

Vol.50 No.4 (July 2023)
Review Article View: 2,024 Download: 928
New Report of Curvularia pseudobrachyspora Causing Brown Spot on Costa Rican Pitahaya Fruit (Hylocereus costaricensis) in Thailand
DOI: 10.12982/CMJS.2023.003.

Fakih Latehnuering, Chaninun Pornsuriya, Prisana Wonglom, On-Uma Ruangwong and Anurag Sunpapao

Vol.50 No.1 (January 2023)
Research Article View: 2,128 Download: 985
Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
DOI: 10.12982/CMJS.2022.109.

Wisan Chuavong, Sirikwan Ponprateep, Pravech Ajawatanawong and Tipachai Vatanavicharn

Vol.49 No.6 (November 2022)
Research Article View: 1,815 Download: 793
History of Thai Mycology and Resolution of Taxonomy for Thai Macrofungi Confused with Europe and American Names
DOI: 10.12982/CMJS.2022.052.

Nakarin Suwannarach, Jaturong Kumla, Surapong Khuna, Nopparat Wannathes, Naritsada Thongklang, Phongeun Sysouphanthong, Thatsanee Luangharn, Sarunyou Wongkanoun, Samantha Chandranath Karunarathna, Yuan-Shuai Liu, Thitiya Boonpratuang, Natthawut Wiriyathanawudhiwong, Rattaket Choeyklin, Kevin D. Hyde and Saisamorn Lumyong

Vol.49 No.3 (Special Issue II : May 2022)
Review Article View: 3,248 Download: 2,137
Endophytic Fungi from Root of Three Lady’s Slipper Orchids (Paphiopedilum spp.) in Southern Thailand
page: 853 - 866

Pornsawan Sutthinon, Kanin Rungwattana, Chalermpol Suwanphakdee, Winanda Himaman and Piyangkun Lueangjaroenkit

Vol.48 No.3 (Special Issue I : May 2021)
Research Article View: 1,429 Download: 990
Prescreening of Ligninolytic Enzyme-Producing Edible White-Rot Fungi
page: 808 - 826

Charuwan Chuaseeharonnachai, Kanthawut Boonmee, Juthamas Suwanprateep, Sayanh Somrithipol, Natapol Pornputtapong and Nattawut Boonyuen

Vol.48 No.3 (Special Issue I : May 2021)
Research Article View: 1,238 Download: 939
Inhibitory Efficacy of Camellia sinensis Leaf and Medicinal Plant Extracts on Helicobacter pylori Standard and Isolate Strains Growth, Urease Enzyme Production and Epithelial Cell Adhesion
page: 56 - 73

Thida Kaewkod, Piyada Wangroongsarb, Itthayakorn Promputtha and Yingmanee Tragoolpua*

Vol.48 No.1 (January 2021)
Research Article View: 1,138 Download: 1,806
Diptoindonesin D, a Potent Antibacterial Activity Against Gram-positive Bacteria, an Inhibitor of Penicillin-binding Protein 2a from the Stem Bark of Shorea roxburghii G.Don
page: 1161 - 1175

Kanokorn Sudto, Patchreenart Saparpakorn, Chompoonuch Tancharoen, Darinee Phromyothin, Supanna Techasakul, Nisachon Khunnawutmanotham, Srunya Vajrodaya, Hui-Ming Ge, Ren Xiang Tan, and Supa Hannongbua*

Vol.46 No.6 (November 2019)
Research Article View: 1,361 Download: 693
Genome-scale Identification and Analysis of Genes Encoding Putative Light-harvesting Chlorophyll a/b binding Proteins in Potato (Solanum tuberosum L.)
page: 867 - 879

Phi Bang Cao*, Thi Thanh Huyen Tran, Van Dinh Nguyen, Viet Hong La and Sahar Azar

Vol.46 No.5 (September 2019)
Research Article View: 1,180 Download: 292
Isolation, Identification and Partial Characterization of Film-forming Microorganisms from Chinese Homemade Pickle, a Traditional Fermented Vegetable Product
page: 2283 - 2293

Qi-Wen Cen, Tao Chen, Wei Zheng, Yu Zhang, Rui-Feng Ying, Zhen-Xing Tang and Lu-E Shi

Vol.45 No.6 (September 2018)
Research Article View: 1,588 Download: 397
Effect of the Thai Traditional Plant Formula, Hing-Hai on Colon Cancer Cell Growth and Metastasis
page: 1745 - 1758

Rujirek Chaiwongsa and Preeyanat Vongchan

Vol.45 No.4 (July 2018)
Research Article View: 982 Download: 363
Geological Interpretation by Peak Spectral Decomposition Envelope Attribute
page: 1549 - 1558

Gritsadapong Leaungvongpaisan*, Pisanu Wongpornchai and Siriporn Chaisri

Vol.45 No.3 (May 2018)
Research Article View: 982 Download: 369
Determination Using Gamma Spectroscopy of Natural Radionuclide Activity Concentrations and Annual Committed Effective Doses in Selected Thai Medicinal Plants
page: 821 - 831

Kiadtisak Saenboonruang*, Endu Phonchanthuek and Kamonkhuan Prasandee

Vol.45 No.2 (March 2018)
Research Article View: 904 Download: 307
Diversity of Endophytic Fungi Isolated from Thai Medicinal Plants (Casearia grewiifolia) in Khon Kaen, Thailand, and their Bioactive Compounds
page: 45 - 59

Natanong Yodsing, Somdej Kanokmedhakul, Wiyada Mongkolthanaruk, Tadanori Aimi, Totsaporn Srisapoomi and Sophon Boonlue

Vol.45 No.1 (January 2018)
Research Article View: 1,422 Download: 672
DNA Barcoding of the Economically Important Leucocalocybe mongolica to Prevent Mushroom Mislabeling
page: 1201 - 1209

Peng Zhao, Wen-Ying Zhuang, Tolgor Bau and Xiao-Dan Yu

Vol.44 No.4 (October 2017)
Research Article View: 974 Download: 434
Antinociceptive and Anti-inflammatory Effects of the Ethanolic Extract of Curcuma aff. amada
page: 912 - 928

Apicha Jabsuwan, Suchada Sukrong, Somporn Swasdison and Pasarapa Towiwat*

Vol.44 No.3 (July 2017)
Research Article View: 912 Download: 327
Discovery of Insertion-deletion Polymorphism for Identification on Catfish Species (Pangasianodon gigas, Pangasianodon hypophthalmus)
page: 756 - 766

Kriangsak Mengumphan [a], Nantaporn Sutthi [b], Doungporn Amornlerdpison[a] and Supamit Mekchay* [b]

Vol.43 No.4 (JULY 2016)
Research Article View: 884 Download: 313
Forensic Soil Investigation from the 16S rDNA Profiles of Soil Bacteria Obtained by Denaturing Gradient Gel Electrophoresis
page: 748 - 755

Anothai Sanachai [a], Somporn Katekeaw [b] and Khemika Lomthaisong [a]*

Vol.43 No.4 (JULY 2016)
Research Article View: 1,012 Download: 462
Efficacy of Houttuynia cordata Lour extracts against herpes simplex virus infection
page: 317 - 330

Pratya Chaliewchalad, Sunee Chansakaow and Yingmanee Tragoolpua

Vol.42 No.2 (APRIL 2015)
Research Article View: 869 Download: 411
Identification and Nutritional Value of Live Feeds for Ornamental Fish from Bangkok Metropolitan Markets in Thailand
page: 364 - 375

Supenya Chittapun* [a], Darawan Ruamkuson [b] and Mariena Ketudat-Cairns* [b,c]

Vol.40 No.3 (JULY 2013)
Research Article View: 946 Download: 269
The study of learnability of the class of k-acceptable languages on Gold’s learning model
page: 248 - 260

Anuchit Jitpattanakul* [a] , and Athasit Surarerks[a]

Vol.40 No.2 (APRIL 2013)
Research Article View: 1,001 Download: 253
In vitro INHIBITION OF LIPID PEROXIDATION AND THE ANTIOXIDANT SYSTEM OF ENDOPHYTIC FUNGI FROM THAI MEDICINAL PLANTS
page: 429 - 444

Teerayut Theantana [a,b], Duangta Kanjanapothi [c], Saisamorn Lumyong* [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,057 Download: 294
Diversity of Endophytic Fungi Associated with Cinnamomum bejolghota (Lauraceae) in Northern Thailand
page: 389 - 398

Nakarin Suwannarach [a], Boonsom Bussaban [a], Wipornpan Nuangmek [b], Eric H.C. McKenzie [c], Kevin

Vol.39 No.3 (JULY 2012)
Research Article View: 958 Download: 360
Outline
Figures