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Effect of the Thai Traditional Plant Formula, Hing-Hai on Colon Cancer Cell Growth and Metastasis
Paper Type  |
Contributed Paper |
Title |
Effect of the Thai Traditional Plant Formula, Hing-Hai on Colon Cancer Cell Growth and Metastasis |
Author |
Rujirek Chaiwongsa and Preeyanat Vongchan |
Email |
preeyanat.v@cmu.ac.th |
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Abstract: A Thai traditional formula named Hing-Hai [L1] is a water extracted mixtures of 7 particular medicinal plants. It is well-known and prescribed to treat 3 major cancers including colon, liver and lung. However, there was no scientific support for its effect and its detailed mechanism remained elusive. This work aimed to evaluate the anti-cancer effect of Hing-Hai and explore its mechanism of action. Hing-Hai was cytotoxic to Caco2 and SW620 in dose-dependent [L2] with IC50 of 234 and 438 µg/ml, respectively by MTT assay. Assay on apoptotic induction and cell cycle analysis using flow cytometry revealed that Hing-Hai did not induce apoptosis or arrest the cell cycle. Studies of cell adhesion in the presence of 3 major adhesion molecules including collagen I, fibronectin, and vitronectin, demonstrated that Hing-Hai inhibited adhesion of both cancer cells in dose-dependent [L3] manner. Scratch wound-healing motility assay and gelatin zymography also confirmed that Hing-Hai could delay cell migration and decrease gelatinolytic activity. Interestingly, at the same concentration, Hing-Hai effectively inhibited adhesion, migration, and decreased the[L4] gelatinolytic activity of SW620 more than that of Caco2 cell. These findings support anti-colon cancer activity of Thai traditional plant formula, Hing-Hai. The studies clearly confirmed anti-cancer activity based on the inhibition of cell adhesion and migration and decreasing the elatinolytic activity. |
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Start & End Page |
1745 - 1758 |
Received Date |
2017-09-24 |
Revised Date |
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Accepted Date |
2018-01-16 |
Full Text |
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Keyword |
Traditional medicinal plant, Anti-metastasis, Anti-cancer activity, Caco2, SW620 |
Volume |
Vol.45 No.4 (July 2018) |
DOI |
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Citation |
Chaiwongsa R. and Vongchan P., Effect of the Thai Traditional Plant Formula, Hing-Hai on Colon Cancer Cell Growth and Metastasis, Chiang Mai Journal of Science, 2018; 45(4): 1745-1758. |
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