Chiang Mai Journal of Science

Physicochemical and Microbial Characteristics of Anaerobic Granule Nuclei Developed by Cationic Polymer Additions under Syntroph Specific Substrate

Paper Type	Contributed Paper
Title	Physicochemical and Microbial Characteristics of Anaerobic Granule Nuclei Developed by Cationic Polymer Additions under Syntroph Specific Substrate
Author	Nasrul Hudayah, Benjaphon Suraraksa and Pawinee Chaiprasert*
Email	pawinee.cha@kmutt.ac.th
Abstract: This study aimed to build syntroph adapted-nuclei in a short period by concomitant early granulation and adaptation of seed sludge under a syntroph-specific substrate. Three UASB reactors – R1 (control-without polymer addition), R2 (dynafloc-synthetic polymer), and R3 (chitosan-natural cationic polymer) – were used. This strategy was successfully achieved in R3 (chitosan addition) on day 58. Chitosan decreased the negativity of microbial surface charges, as represented by the increased zeta potential from -26.4 mV at day 0 to -10.5 mV at day 58. The negativity of microbial surface charges in R1 and R2 slightly decreased to -24.5 mV and -22.2 mV on day 58, respectively. As a result, nuclei ratio and mean diameter size of microbial aggregates in R3 were significantly higher by approximately 55.1% and 115 µm, respectively, compared to those in R1 and R2, which then led to low SVI value. No significant difference in EPS production was found between the reactors, indicating that early granulation was mainly affected by the addition of cationic polymers, especially chitosan. However, microbial adaptation to the syntroph-specific substrate during the nucleation phase effectively increased specific activities of selected syntrophic microorganisms in all reactors. Microbial self-aggregation occurred in microbial aggregates of R1 due to the absence of cationic polymer additions. The microbial distributions, i.e., bacteria and Archaea, were well observed in aggregates of R3.
Start & End Page	102 - 114
Received Date	2015-12-25
Revised Date
Accepted Date	2016-03-02
Full Text	Download
Keyword	nucleation, adaptation, syntroph-specific substrate, zeta potential, cationic polymers
Volume	Vol.44 No.1 (JANUARY 2017)
DOI
Citation	Hudayah N., Suraraksa B. and Chaiprasert P., Physicochemical and Microbial Characteristics of Anaerobic Granule Nuclei Developed by Cationic Polymer Additions under Syntroph Specific Substrate, Chiang Mai J. Sci., 2017; 44(1): 102-114.
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