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Simple and Efficient Method for the Detection and Quantification of Cordycepin Content in Cordyceps

Paper Type 
Contributed Paper
Simple and Efficient Method for the Detection and Quantification of Cordycepin Content in Cordyceps
Doan Thi Phuong Thuy*, Tran Thi Ngoc Anh, Nguyen Thi Thanh Thuy, Phikul Intaparn, Tawat Tapingkae and Nguyen Thi Mai

Cordycepin content is an essential parameter that defines the medicinal quality of Cordyceps.

However, most Cordyceps growers cannot conduct routine assessment of cordycepin due to the
equipment cost and intensive time of the analysis method.
In this report, we developed an analysis method based on thin layer chromatography that could
be efficiently used as an alternative to other high-tech method for the detection and quantification of
cordycepin content. Moreover, in our analyses, eco-friendly chemicals were also used so that Cordyceps
growers could safely utilize this method for routine quality control of cordycepin content.
Our TLC analysis showed that the developing solvents of ethyl acetate:methanol:water, 100:15:10
(v/v/v) was the optimal mobile phase for the separation of cordycepin from adenosine and its analogs.
Over the tested concentrations of cordycepin (0.5-6.0 μg), there was a linear relationship between the
cordycepin contents and the optical densities of the spots on TLC plate. The DL and QL values were
calculated as 0.14 μg and 0.43 μg/spot, respectively. The relative standard deviations (RSD) between
analyses were remained lower than 4%. The accuracy tests showed that the recovered amounts of
cordycepin were 97.19% to 102.04%.
This method was therefore suitable for the quantification of cordycepin content in Cordyceps.
Start & End Page 
420 - 428
Received Date 
Revised Date 
Accepted Date 
Full Text 
Cordyceps, cordycepin, eco-friendly, quantification, thin layer chromatography
Vol.48 No.2 (March 2021)
Thuy D.T.P., Anh T.T.N., Thuy N.T.T., Intaparn P., Tapingkae T. and Mai N.T., Simple and Efficient Method for the Detection and Quantification of Cordycepin Content in Cordyceps, Chiang Mai J. Sci., 2021; 48(2): 420-428.
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Effects of Water Content on Compressive Strength of Ecofriendly Light-weight Cement Blocks Using Cement-like Material Prepared from Agricultural Wastes
page: 700 - 701
Author:Latda Chandeng, Thanawat Meesak, Chakrapan Tuakta, Tidarut Jirawattanasomkul, Tamon Ueda and Oratai Jongprateep*
Vol.47 No.4 (Special Issue II : July 2020) View: 928 Download:414
Enhanced Production of Cordycepin by Solid State Fermentation of Cordyceps militaris using Additives
page: 972 - 984
Author:Ting-Chi Wen [a]+, Chao Kang [b]+, Ze-Bing Meng [a], Yong-Bin Qi [a], Kevin D. Hyde [c, d] and Ji-Chuan Kang* [a]
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Mosquito Larvicidal Properties of Anisomeles Malabarica (L.) Extracts Against the Malarial Vector, Anopheles Stephensi (Liston)
page: 148 - 155
Author:Yuwalee Unpaprom [a], Rameshprabu Ramaraj *[b] and Murugan Kadarkarai [b]
Vol.42 No.1 (JANUARY 2015) View: 494 Download:191
Optimization of Solid-state Fermentation for Fruiting Body Growth and Cordecepin Production by Cordyceps militaris
page: 858 - 872
Author:Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde
Vol.41 No.4 (SPECIAL ISSUE 1) View: 626 Download:476
Phenotypic Marking of Cordyceps militaris Fruiting-Bodies and Their Cordycepin Production
page: 846 - 857
Author:Ting-chi Wen, Ji-chuan Kang*, Kevin D. Hyde, Guang-rong Li, Chao Kang and Xu Chen
Vol.41 No.4 (SPECIAL ISSUE 1) View: 610 Download:226
High Performance Thin Layer Chromatographic Method for the Determination of Diclofenac Sodium in Pharmaceutical Formulations
page: 123 - 128
Author:Wisanu Thongchai, Boonsom Liawruangrath*, Chalermporn Thongpoon and Theeraphan Machan
Vol.33 No.1 (JANUARY 2006) View: 563 Download:158

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