A Comparative Study on Simultaneous Saccharification and Fermentation of Agricultural Wastes to Bio-ethanol Using Yeast Strains
Poludasu Rama Mohan, Sake Pradeep and Obulam Vijaya Sarathi Reddy** Author for corresponding; e-mail address: ovsreddy@yahoo.com
Volume: Vol.40 No.3 (JULY 2013)
Research Article
DOI:
Received: 23 Febuary 2012, Revised: -, Accepted: 20 September 2012, Published: -
Citation: Mohan P.R., Pradeep S. and Reddy O.V.S., A Comparative Study on Simultaneous Saccharification and Fermentation of Agricultural Wastes to Bio-ethanol Using Yeast Strains, Chiang Mai Journal of Science, 2013; 40(3): 307-320.
Abstract
Application of simultaneous saccharification and fermentation (SsF) technique for the conversion of agricultural wastes to bio-ethanol would result in a more cost-effective process. Peracetic acid (PAA) pretreated various lignocellulosic substrates like sugarcane bagasse, rice straw and corn cobs were examined with solka floc as reference in SsF experiments for the production of bio-ethanol using Asperigillus niger crude cellulase, Cladosporium sp. crude CMCase and yeast cells. Fermentation temperature was selected as 40 and 43ºC for Saccharomyces cerevisiae NCIM 3215 and Saccharomyces bayanusrespectively were compared in SsF experiments.The S. bayanus was found to perform better and faster fermentation in the SsF process and yielded higher bio-ethanol in pretreated bagasse just after 48 h (17.03% w/v) than the S. cerevisiae that gave high yields of bio-ethanol only after 72 h (13.44% w/v). Increased bio-ethanol yields were obtained when the cellulase was supplemented with CMCase of Cladosporium sp. The cell viability was lower in S. bayanus compared with S. cerevisiae. The bio-ethanol yields of the fermented substrates were in the following order:Solka floc> treated sugarcane bagasse> treated rice straw > treated corn cobs.