Chiang Mai Journal of Science

A Comparative Study on Simultaneous Saccharification and Fermentation of Agricultural Wastes to Bio-ethanol Using Yeast Strains

Paper Type	Contributed Paper
Title	A Comparative Study on Simultaneous Saccharification and Fermentation of Agricultural Wastes to Bio-ethanol Using Yeast Strains
Author	Poludasu Rama Mohan, Sake Pradeep and Obulam Vijaya Sarathi Reddy*
Email	ovsreddy@yahoo.com
Abstract:  Application of simultaneous saccharification and fermentation (SsF) technique for the conversion of agricultural wastes to bio-ethanol would result in a more cost-effective process. Peracetic acid (PAA) pretreated various lignocellulosic substrates like sugarcane bagasse, rice straw and corn cobs were examined with solka floc as reference in SsF experiments for the production of bio-ethanol using Asperigillus niger crude cellulase, Cladosporium sp. crude CMCase and yeast cells. Fermentation temperature was selected as 40 and 43ºC for Saccharomyces cerevisiae NCIM 3215 and Saccharomyces bayanusrespectively were compared in SsF experiments.The S. bayanus was found to perform better and faster fermentation in the SsF process and yielded higher bio-ethanol in pretreated bagasse just after 48 h (17.03% w/v) than the S. cerevisiae that gave high yields of bio-ethanol only after 72 h (13.44% w/v). Increased bio-ethanol yields were obtained when the cellulase was supplemented with CMCase of Cladosporium sp. The cell viability was lower in S. bayanus compared with S. cerevisiae. The bio-ethanol yields of the fermented substrates were in the following order:Solka floc> treated sugarcane bagasse> treated rice straw > treated corn cobs.
Start & End Page	307 - 320
Received Date	2012-02-23
Revised Date
Accepted Date	2012-09-20
Full Text	Download
Keyword	simultaneous saccharification and fermentation (SSF), Cladosporium sp., Asperigillus niger, lignocellulose, Saccharomyces cerevisiae, Saccharomyces bayanus, bio-ethanol
Volume	Vol.40 No.3 (JULY 2013)
DOI
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