In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts
Raghavendra H. Lakshmanashetty, Vijayananda B. Nagaraj, Madhumathi G. Hiremath and Vadlapudi Kumar* Author for corresponding; e-mail address: kvadlapudi@rediffmail.com
Volume: Vol.37 No.3 (SEPTEMBER 2010)
Research Article
DOI:
Received: 27 Febuary 2010, Revised: -, Accepted: 5 May 2010, Published: -
Citation: Lakshmanashetty R.H., Nagaraj V.B., Hiremath M.G. and Kumar V., In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts , Chiang Mai Journal of Science, 2010; 37(3): 489-497.
Abstract
In the present study, Vitex negundo L. leaf extracts were screened for their phytochemical composition and antioxidant activity. The yield of phytochemicals is in the order of ethanol extract > methanol extract > benzene extract > chloroform extract > hexane extract > ethyl acetate extract > petroleum extract. Finally we have selected ethanol extract for the antioxidant activity. Total phenolic content was estimated as gallic acid equivalents (249.96 +/- 8.34 mg/g of extract) and total flavonoids estimated as catechin equivalents (166.67 +/- 9.14 mg/g of extract). Free radical scavenging activity was determined by DPPH assay (79.82 +/- 2.99% at 500 μg/ml concentration). Total antioxidant capacity of the extract (214.81 +/- 4.07 mg ascorbic acid equivalent/g) was determined. Ferrous chelating ability was found to be 84.15 +/- 1.65% at 500 μg/ml concentration and reductive ability of the extract was found to be 0.918 0.035 absorbance unit at 600 μg/ml concentration. The antioxidant activity of Vitex negundo L. is mainly due to the presence of the phenolic compounds like, flavonoids and flavonols.
Related Articles
DOI: 10.12982/CMJS.2024.035.
Warunee Chomkitichai and Sitthisak Intarasit
Vol.51 No.3 (May 2024)Research Article View: 1,724 Download: 1,003
page: 405 - 419
Mohd Hafiz Ahmad, Wageeh A. Yehye, Noorsaadah Abd. Rahman, Lina A. Al-Ani, Mohd Rafie Johan, Jun Lu and Najihah M. Hashim*
Vol.48 No.2 (March 2021)Research Article View: 1,937 Download: 542
page: 295 - 307
Puttaporn Klayprasert, Kritsana Jitmanee, Napaporn Youngvises and Jaroon Jakmunee*
Vol.46 No.2 (March 2019)Research Article View: 1,008 Download: 340
page: 72 - 92
Qingzhi Ding, Ricardo Antonio Wu-Chen, Qi Wu, Hanfei Jiang, Ting Zhang, Lin Luo, Haile Ma*, Shiqiao Ma and Ronghai He
Vol.46 No.1 (January 2019)Research Article View: 1,049 Download: 439
page: 897 - 904
Tarek F. Eissa, Elena Gonzalez-Burgos, M. Emilia Carretero and M. Pilar Gomez-Serranillos*
Vol.45 No.2 (March 2018)Research Article View: 998 Download: 319
page: 888 - 896
Hemant A. Sawarkar*, Pranita P. Kashyap and Chanchal Deep Kaur
Vol.45 No.2 (March 2018)Research Article View: 850 Download: 359
page: 881 - 887
Farman Ali, Abdul Khaliq Jan, Nasir Mehmood Khan, Rahamdad Ali, Muhammad Mukhtiar, Shahnaz Khan, Seemab Ali Khan and Riffat Aziz
Vol.45 No.2 (March 2018)Research Article View: 995 Download: 285
page: 868 - 880
Yongyuth Theapparat*, Chanita Ponglimanont, Ausa Chandumpai and Nikhom Laemsak
Vol.45 No.2 (March 2018)Research Article View: 926 Download: 364
page: 427 - 439
Biying Wang, Chloe Desiree Goldsmith, Jiahong Zhao, Shanshan Zhao, Zunlai Sheng and Wenhui Yu
Vol.45 No.1 (January 2018)Research Article View: 2,149 Download: 317
page: 331 - 341
Qian Chen, Tian-Tian Zhang and Jian-Guo Jiang
Vol.45 No.1 (January 2018)Research Article View: 992 Download: 280
page: 287 - 298
Duygu Taşkın, Dilek B. Alkaya and Emre Dölen
Vol.45 No.1 (January 2018)Research Article View: 1,644 Download: 318
page: 946 - 955
Natcha Laokuldilok, Prodpran Thakeow, Phikunthong Kopermsub and Niramon Utama-ang*
Vol.44 No.3 (July 2017)Research Article View: 1,089 Download: 378
page: 929 - 938
Shruti Shukla* and Archana Mehta
Vol.44 No.3 (July 2017)Research Article View: 1,113 Download: 474
page: 123 - 137
Pornnapa Chuenchom, Prasan Swatsitang, Thanaset Senawong, Sanun Jogloy
Vol.43 No.1 (JANUARY 2016)Research Article View: 1,106 Download: 594
page: 886 - 895
Shivraj Hariram Nile, Umesh Bapurao Patil and Se Won Park
Vol.42 No.4 (OCTOBER 2015)Research Article View: 1,038 Download: 514
page: 156 - 172
Meltem Asan Ozusaglam, Mahmut Erzengin, Derya Onal Darilmaz, Seher Karaman Erkul, Mehtap Tekse
Vol.42 No.1 (JANUARY 2015)Research Article View: 1,086 Download: 246
page: 173 - 184
Kah Weng Chua [a], Chiaw Mei Sia [a], Gabriel A. Akowuah [b], Loshnie Samuagam [a], and Hip Seng Yim
Vol.42 No.1 (JANUARY 2015)Research Article View: 1,026 Download: 445
page: 1241 - 1249
Nipaporn Sengkhamparn* and Nutchanat Phonkerd
Vol.41 No.5/2 (OCTOBER 2014)Research Article View: 1,002 Download: 335
page: 636 - 642
Nattaya Ongphimai [a], Supathra Lilitchan*[a], Kornkanok Aryusuk [b], Akkarach Bumrungpert [a], et a
Vol.40 No.4 (OCTOBER 2013)Research Article View: 1,050 Download: 366
page: 224 - 231
Jakkrapun Kongkedsuk [a], Apisake Hongwitayakorn [a], Wannarekha Bootnapang [b], Nisachon Boonto [b
Vol.40 No.2 (APRIL 2013)Research Article View: 1,426 Download: 338
page: 49 - 59
Bibi Sedigheh Fazly-Bazzaz *[a], Atefeh Arab [b], Seyed Ahmad Emami [c], Javad Asili [c], Mohamad Ha
Vol.40 No.1 (JANUARY 2013)Research Article View: 1,007 Download: 333
page: 117 - 122
Sarunya Chuanphongpanich* [a], Maitree Suttajit [b], Sukon Phanichphant [c Duang Buddhasukh [c]
Vol.33 No.1 (JANUARY 2006)Research Article View: 998 Download: 367