Chiang Mai Journal of Science

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In the present study, Vitex negundo L. leaf  extracts were screened for their phytochemical composition and antioxidant activity. The yield of  phytochemicals is in the order of  ethanol extract > methanol extract > benzene extract > chloroform extract > hexane extract > ethyl acetate extract > petroleum extract. Finally we have selected ethanol extract for the antioxidant activity. Total phenolic content was estimated as gallic acid equivalents (249.96 +/- 8.34 mg/g of extract) and total flavonoids estimated as catechin equivalents (166.67 +/- 9.14 mg/g of extract). Free radical scavenging activity was determined by DPPH assay (79.82 +/- 2.99% at 500 μg/ml concentration). Total antioxidant capacity of  the extract (214.81 +/- 4.07 mg ascorbic acid equivalent/g) was determined. Ferrous chelating ability was found to be 84.15 +/- 1.65% at 500 μg/ml concentration and reductive ability of the extract was found to be 0.918  0.035 absorbance unit at 600 μg/ml concentration. The antioxidant activity of Vitex negundo L. is mainly due to the presence of  the phenolic compounds like, flavonoids and flavonols.

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