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In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts
Paper Type  |
Contributed Paper |
Title |
In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts |
Author |
Raghavendra H. Lakshmanashetty, Vijayananda B. Nagaraj, Madhumathi G. Hiremath and Vadlapudi Kumar |
Email |
kvadlapudi@rediffmail.com |
|
Abstract: In the present study, Vitex negundo L. leaf extracts were screened for their phytochemical composition and antioxidant activity. The yield of phytochemicals is in the order of ethanol
extract > methanol extract > benzene extract > chloroform extract > hexane extract > ethyl acetate extract > petroleum extract. Finally we have selected ethanol extract for the antioxidant activity. Total phenolic content was estimated as gallic acid equivalents (249.96 +/- 8.34 mg/g of extract) and total flavonoids estimated as catechin equivalents (166.67 +/- 9.14 mg/g of extract). Free radical scavenging activity was determined by DPPH assay (79.82 +/- 2.99% at 500 μg/ml concentration). Total antioxidant capacity of the extract (214.81 +/- 4.07 mg ascorbic acid equivalent/g) was determined. Ferrous chelating ability was found to be 84.15 +/- 1.65% at 500 μg/ml concentration and reductive ability of the extract was found to be 0.918 0.035 absorbance unit at 600 μg/ml concentration. The antioxidant activity of Vitex negundo L. is mainly due to the presence of the phenolic compounds like, flavonoids and flavonols.
|
|
Start & End Page |
489 - 497 |
Received Date |
2010-02-27 |
Revised Date |
|
Accepted Date |
2010-05-05 |
Full Text |
Download |
Keyword |
Vitex negundo L, DPPH, Reducing power, Phenols, Antioxidant capacity, Flavonoids |
Volume |
Vol.37 No.3 (SEPTEMBER 2010) |
DOI |
|
Citation |
Lakshmanashetty R.H., Nagaraj V.B., Hiremath M.G. and Kumar V., In vitro Antioxidant Activity of Vitex negundo L. Leaf Extracts , Chiang Mai J. Sci., 2010; 37(3): 489-497. |
SDGs |
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| View:656 Download:390 | |
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