Chiang Mai Journal of Science

Antioxidant and Cytotoxicity Activities of Butylated Hydroxytoluene Ligands Capped Gold Nanoparticles

Paper Type	Contributed Paper
Title	Antioxidant and Cytotoxicity Activities of Butylated Hydroxytoluene Ligands Capped Gold Nanoparticles
Author	Mohd Hafiz Ahmad, Wageeh A. Yehye, Noorsaadah Abd. Rahman, Lina A. Al-Ani, Mohd Rafie Johan, Jun Lu and Najihah M. Hashim*
Email	najihahmh@um.edu.my
Abstract: The new potent nano-antioxidant of sulfur-containing butylated hydroxytoluene ligands (S-BHTLs)-conjugated with gold nanoparticles, Au-S-BHTLs, was synthesized by a conjugation of sulfur-containing ligands derived from BHT on the surface of gold nanoparticles (AuNPs). The in-house developing eight sulfur-containing BHT-ligands (S-BHTLs) were used for further study on functionalization with AuNPs and their biological activities. The antioxidant and cytotoxic abilities of eight types of Au-S-BHTLs such as compound Au-2a, Au-2b, Au-3a, Au-3b, Au-4a, Au-4b, Au-5a and Au-5b were tested by DPPH• (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and MTT assays against HT29 (human colorectal adenocarcinoma) and MCF7 (human breast adenocarcinoma). Based on the results, it was revealed that nanocomposites Au-5a and Au-3a attained over 50% lower IC50 values against free radical than the un-functionalized AuNPs. Meanwhile, nanocomposite Au-2b showed the highest cytotoxicity effect against both cancer cell lines. Selectivity and safety towards non-tumorgenic cell lines were also evaluated and proved superior selectivity index (SI) of all nanocomposites (SI > 2.00) against both cancer cells except Au-5b (SI = 1.87) against MCF7. Hence, the functionalization of S-BHTLs with gold nanoparticles has increased the selectivity of nanocomposites and enhanced the antioxidant potentials to become useful and promising anticancer agents.
Start & End Page	405 - 419
Received Date	2020-09-16
Revised Date
Accepted Date	2020-11-20
Full Text	Download
Keyword	gold nanoparticles, Au-S-BHTLs, DPPH, HT29, MCF7
Volume	Vol.48 No.2 (March 2021)
DOI
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