Optimization of Ultrasonic-assisted Extraction Using Taguchi Design and In vivo Activities ofPolysaccharides from Flos populi

Ying Zhang, Jiaxuan Li, Zheng Jia, Jianqing Chen, Chunbo Gao and Zunlai Sheng
* Author for corresponding; e-mail address: shengzunlai@neau.edu.cn
Volume: Vol.46 No.3 (May 2019)
Research Article
DOI:
Received: 2 August 2018, Revised: -, Accepted: 24 October 2018, Published: -

Citation: Zhang Y., Li J., Jia Z., Chen J., Gao C. and Sheng Z., Optimization of Ultrasonic-assisted Extraction Using Taguchi Design and In vivo Activities ofPolysaccharides from Flos populi, Chiang Mai Journal of Science, 2019; 46(3): 510-522.

Abstract

There is an increasing demand for developing simple and efficient extraction of active polysaccharides from herbs, and ultrasound-assisted extraction (UAE) has attracted considerable interest. In this study, UAE of polysaccharides from Flos populi (PFP) was optimized using Taguchi L₂₇ orthogonal design. The statistical analysis showed that the most important factors contributing to the extraction yield of PFP were liquid to solid ratio, extraction temperature, ultrasonic power and time, respectively, and the optimum conditions were at 25 mL/g, 50 ^°C, 400 W and 30 min which result the maximum yield of PFP (59.8 mg/g). At the above conditions, the extraction yield of 61.9 ± 2.5 mg/g was close to the predicted value. Moreover, the effect of extraction cycle (1-5) on the PFP yield was tested, and extraction cycle of 2 was determined. The UAE and conventional solvent extraction methods (CSE) were compared for extracting PFP. The UAE technique was very efficient in the extraction of PFP compared with CSE, for the similar yield of PFP was obtained at 60 and 150 min, respectively. Additionally, the antioxidant activities of PFP were accessed in-vivo. The results revealed that PFP had markedly enhanced the levels of catalase(CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), while decreased the level of malonaldehyde (MDA) in serums and livers of mice compared to CCl₄treated group. The study revealed that PFP possess potent antioxidant activity and could be useful for the development of new antioxidant agents.

Keywords: Flos populi, polysaccharides, ultrasonic-assisted extraction, taguchi experimental design, antioxidant activity

Development and Evaluation of Buckwheat-Fortified Mung Bean Extrudates: Effects on Physicochemical, Textural, and Antioxidant Properties
DOI: 10.12982/CMJS.2025.100.

Girish Ningappa Mathad and Jenshinn Lin

Vol.52 No.6 (November 2025)
Research Article View: 299 Download: 65

Exploring The Health Benefits of Ganoderma; Hepatoprotective and Cardiovascular Effects and Mechanisms of Action
DOI: 10.12982/CMJS.2025.028.

Samantha Chandranath Karunarathna, Nimesha Patabendige and Kalani Kanchana Hapuarachchi

Vol.52 No.3 (May 2025)
Review Article View: 856 Download: 201

Exploring the Effect of Incubation pH, Temperature, and In Vitro Digestion on Antioxidant Potential of Mung Bean Protein Hydrolysates
DOI: 10.12982/CMJS.2024.090.

Zohreh Karami and Kiattisak Duangmal

Vol.51 No.6 (November 2024)
Research Article View: 902 Download: 306

Effects of Simulated Digestion on the Bioaccessibility of Phenolic Compounds and Antioxidant Activity of Dehydrated Bignay [Antidesma bunius (L.) Spreng]
DOI: 10.12982/CMJS.2024.032.

Ara Fatima A. Carbonera, Liezl M. Atienza, Maria Amelita C. Estacio, Sheba Mae M. Duque, Rona Camille M. Lizardo-Agustin, and Katherine Ann T. Castillo-Israel

Vol.51 No.2 (March 2024)
Research Article View: 1,391 Download: 481

Effects of Aqueous Extraction Factors on Commercial Protease Inhibitory Activities, Phenolic Acid Profiles and Chemical Properties of Hom Mali-105 Rice (Oryza sativa L.) Bran Extract
DOI: 10.12982/CMJS.2023.054.

Worachai Wongwatcharayothin, Supawan Thawornchinsombut and Akkasit Jongjareonrak

Vol.50 No.5 (September 2023)
Research Article View: 1,504 Download: 942

Efficacy of Dried Indian Gooseberry Kombucha on a Pre–industrial Scale: Chemical Composition, Antioxidant and Antibacterial Activity
DOI: 10.12982/CMJS.2023.034.

Tharinee Klawpiyapamornkun, Thida Kaewkod, Toungporn Uttarotai, Sunanta Wangkarn, Panee Sirisa-ard, Suwalee Kiatkarun, Yingmanee Tragoolpua and Sakunnee Bovonsombut

Vol.50 No.3 (May 2023)
Research Article View: 1,325 Download: 801

Salicylic Acid Elicitor Inhibiting Xanthomonas oryzae Growth, Motility, Biofilm, Polysaccharides Production, and Biochemical Components during Pathogenesis on Rice
page: 341 - 353

Wannaporn Thepbandit, Natthiya Buensanteai, Kanjana Thumanu, Supatcharee Siriwong, Toan Le Thanh and Dusit Athinuwat

Vol.48 No.2 (March 2021)
Research Article View: 2,006 Download: 1,303

Potent Antioxidant Activities of Half-Sib Families of Eucalyptus camaldulensis Dehnh. Leaf Essential Oils Planted in Thailand and Their Antioxidative Components
page: 112 - 122

Sapit Diloksumpun, Preeya Jeenho, Samran Namkhot, Tharinee Saleepochn, Suwaporn Luangkamin*

Vol.48 No.1 (January 2021)
Research Article View: 1,336 Download: 547

Multi-response Optimization of Ultrasonic Assisted Enzymatic Extraction of Antioxidant Polysaccharides from Waste Ginkgo biloba Sarcotesta
page: 499 - 512

Gui-Sheng Zhou, Yu-Ping Tang, Yao Li and Jin-Ao Duan

Vol.47 No.3 (May 2020)
Research Article View: 1,238 Download: 2,104

Solvent Extraction and Measurement of Antioxidant Activity and Total Phenolic Content from Capsicum chinense Jacq. Cv Habanero at Different Maturity Stages
page: 661 - 671

Víctor Manuel Moo-Huchin*, María de Lourdes Vargas y Vargas , Jorge Abraham Tamayo-Cortez, Daniel Arcángel López-Sauri, Enrique Sauri-Duch, Alejandro Ortiz-Fernández, César Abraham Can-Cauich, David Betancur-Ancona

Vol.46 No.4 (July 2019)
Research Article View: 1,121 Download: 446

Antibacterial, Antioxidant Properties and Bioactive Compounds of Thai Cultivated Mushroom Extracts Against Food-borne Bacterial Strains
page: 1713 - 1727

Mathurot Chaiharn, Waya S. Phutdhawong, Doungporn Amornlerdpison and Weerachai Phutdhawong

Vol.45 No.4 (July 2018)
Research Article View: 1,243 Download: 542

Antibacterial and Antioxidant Activities of 3-O-methyl Ellagic Acid from Stem Bark of Polyalthia longifolia Thw.
page: 858 - 867

Pankaj Kumar Jain, Arjun Patra*+, Swaha Satpathy, Sonika Jain and Saleemulla Khan

Vol.45 No.2 (March 2018)
Research Article View: 1,134 Download: 365

Optimization of Ultrasound-assisted Extraction of Quercetin, Luteolin, Apigenin, Pinocembrin and Chrysin from Flos populi by Plackett-burman Design Combined with Taguchi Method
page: 427 - 439

Biying Wang, Chloe Desiree Goldsmith, Jiahong Zhao, Shanshan Zhao, Zunlai Sheng and Wenhui Yu

Vol.45 No.1 (January 2018)
Research Article View: 2,192 Download: 317

Antioxidant Activity and Starch-digesting Enzyme Inhibition of Selected Thai Herb Extracts
page: 263 - 276

Krittalak Pasakawee, Somdet Srichairatanakool, Thunnop Laokuldilok and Niramon Utama-ang

Vol.45 No.1 (January 2018)
Research Article View: 1,143 Download: 293

Carotenoid Composition and Antioxidant Activity of Extracts From Tropical Fruits
page: 605 - 616

V ctor M. Moo-Huchin [a], Gustavo A. Gonz lez-Aguilar [b], Mariela Moo-Huchin [c], Elizabeth Ortiz-V zquez [d], Luis Cuevas-Glory [d], Enrique Sauri-Duch [d] and David Betancur-Ancona* [e]

Vol.44 No.2 (April 2017)
Research Article View: 1,064 Download: 1,054

Antioxidant and Antimicrobial Properties of a Brown Alga Scytosiphon lomentaria
page: 595 - 604

Xiao Juan Xu, Yu Zhang, Hong Lin, Chao Kang, Xiao Ting Fu*

Vol.44 No.2 (April 2017)
Research Article View: 1,072 Download: 277

Antioxidant Activities and Stability of Seed Kernel Extracts from Mango (Mangifera indica linn.) Cultivated in Northern Thailand
page: 573 - 583

Nichana Nakpanich, Wantida Chaiyana and Pimporn Leelapornpisid*

Vol.44 No.2 (April 2017)
Research Article View: 1,167 Download: 401

Phytochemical Screening, Phenolic and Flavonoid Contents, Antioxidant and Cytotoxic Activities of Graptophyllum pictum (L.) Griff.
page: 193 - 202

Nadechanok Jiangseubchatveera, Saisunee Liawruangrath, Aphiwat Teerawutgulrag, Dammrong Santiarwor

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,286 Download: 687

Polyphenol Contents, Antioxidant and Anticancer Activity (MCF-7) of Soybean Products in Thailand
page: 176 - 183

Thidarat Somdee *, Udomsak Mahaweerawat, Jindawan Wibulutai, Namphuang Dungkokruad and Suneerat Yung

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,152 Download: 1,909

Ultrasonic Extraction, Antioxidant and Anti-glycation Activities of Polysaccharides from Gynura divaricata Leaves
page: 144 - 156

Pawinee Deetae*[a, e], Kwanrudee Tseng [a], Chanathinart Pasuphan [a], Atikorn Panya [b], Pawadee Methacanon [c], Waranya Temthawee [a] and Manop Suphantharika [d]

Vol.44 No.1 (JANUARY 2017)
Research Article View: 2,327 Download: 413

Determination of Antioxidant and Anticancer Activities together with Total Phenol and Flavonoid Contents of Cleidion javanicum Bl. and Bridelia retusa (L.) A. Juss.
page: 535 - 546

Duangsuree Sanseera, Boonsom Liawruangrath *, Stephen G. Pyne and Saisunee Liawruangrath*

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,544 Download: 867

Optimization of the Preparation of Gelatin Hydrolysates with Antioxidative Activity from Lizardfish (Saurida spp.) Scales Gelatin
page: 68 - 79

Sutee Wangtueai [a, b]*, Susanne Siebenhandl-Ehn [c] and Dietmar Haltrich [c]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,226 Download: 559

Comparative Studies on Chemical Composition, Phenolic Compounds and Antioxidant Activities of Brown and White Perilla (Perilla frutescens) Seeds
page: 896 - 906

Sikeret Kongkeaw, Siriporn Riebroy and Manat Chaijan

Vol.42 No.4 (OCTOBER 2015)
Research Article View: 1,102 Download: 349

Determination of the Effects of Adding Milk and Sugar on the Antioxidant Capacity of Oolong Tea by Chemical and Cell Culture-Based Antioxidant Assays
page: 699 - 711

Angkana Wipatanawin, Sudarat Phongsawanit, Thadthanan Maneeratprasert, Sittiwat Lertsiri

Vol.42 No.3 (JULY 2015)
Research Article View: 1,209 Download: 377

Fatty Acid Composition, Physical Properties, Acute Oral Toxicity and Antioxidant Activity of Crude Lipids from Adipose Tissue of Some Commercialized Freshwater Catfish
page: 626 - 636

Wiwat Wangcharoen, Kriangsak Mengumphan and Doungporn Amornlerdpison

Vol.42 No.3 (JULY 2015)
Research Article View: 1,030 Download: 265

Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
page: 1 - 16

Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,079 Download: 311

In vitro Antioxidant and Antitumor Activities of an Endophytic Fungus Phomopsis liquidambari QH4 from Artemisia annua
page: 992 - 1006

Yixin Qian [a, b], Jichuan Kang*[a, c], Lu Wang [a], Bangxing Lei [a] and Kevin D. Hyde [d, e]

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,067 Download: 305

Physical Characteristics and Biological Activities of Thai Flower Extracts Loaded in Niosomes
page: 603 - 617

Aranya Manosroi*[a,b], Bang-on Kietthanakorn [b], Charinya Chankhampan [b], Narinthorn Khositsuntiwo

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 1,068 Download: 361

Antimicrobial and Antioxidant Activities of Methanol, Dichloromethane and Ethyl Acetate Extracts of Scutellaria lindbergii Rech.f.
page: 49 - 59

Bibi Sedigheh Fazly-Bazzaz *[a], Atefeh Arab [b], Seyed Ahmad Emami [c], Javad Asili [c], Mohamad Ha

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,044 Download: 335

Antioxidant Potential of Pleurotus porrigens Extract and Application in Sunflower Oil during Accelerated Storage
page: 34 - 48

Hip Seng Yim*[a, b], Fook Yee Chye [b], Mei Lian Liow [a], and Chun Wai Ho [a]

Vol.40 No.1 (JANUARY 2013)
Research Article View: 1,070 Download: 359

Nutritional and Physicochemical Qualities of Thua Nao (Thai Traditional Fermented Soybean)
page: 562 - 574

Katekan Dajanta1,2, Ekachai Chukeatirote3,* and Arunee Apichartsrangkoon2

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 1,017 Download: 344

Antioxidant, Antibacterial, Anticancer Activities and Chemical Constituents of the Essential Oil from Mesua ferrea Leaves
page: 455 - 463

Sukanya Keawsa-ard* [a] and Samart Kongtaweelert [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,125 Download: 627

Antioxidant and Anti-inflammatory Activities of Brown Marine Alga, Padina minor Yamada
page: 507 - 516

Yuwadee Peerapornpisal, Doungporn Amornlerdpison, Utan Jamjai, Tawat Taesotikul, Yanee Pongpaibul,

Vol.37 No.3 (SEPTEMBER 2010)
Research Article View: 1,096 Download: 375

Chiang Mai Journal of Science

1,647

Q3 0.80

Q3 1.3

7 days

Outline

Figures and Tables