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Screening and Identification of Genes Related to Fibroblast Growth Factor 5 Hair Growth Regulation of the Liaoning cashmere Goat


Paper Type 
Contributed Paper
Title 
Screening and Identification of Genes Related to Fibroblast Growth Factor 5 Hair Growth Regulation of the Liaoning cashmere Goat
Author 
Mei Jin, Zi-wei Zhao, Jing-ai Piao, Rui Wang, Jun Piao and Feng-qin Zhao
Email 
jm6688210@163.com.
Abstract:

This study was conducted to screen genes related to hair growth of the Liaoning cashmere goats with fibroblast growth factor 5 (FGF5) treatments and study the molecular regulatory mechanisms of cashmere growth. FGF5 of different concentrations was used to treat skin fibroblasts of the Liaoning cashmere goats over several periods, and the total RNA of the fibroblasts was extracted. Subsequently, the expressed genes were screened by high-throughput sequencing, and the target genes were verified by qPCR. RNA-seq analysis showed that in the F4_24H, F4_72H, and F6_24H groups, 164, 189 and 123 transcripts were expressed in different amounts compared with the control group (group C). qPCR verification showed that the expression levels of OPN and ITGAV genes were up-regulated, which was consistent with the gene-sequencing analysis results obtained from F4_24H vs C group. The CCK-8 results showed that cell proliferation was the most pronounced in the F4_24H group. Therefore, FGF5 is verified to promote the proliferation of skin fibroblasts of Liaoning cashmere goats. Besides, in the F4_24H group, the OPN gene and its downstream ITGAV gene were demonstrated to enhance villus growth by regulating the ECM signaling pathway.

Start & End Page 
1301 - 1312
Received Date 
2020-10-30
Revised Date 
2021-05-11
Accepted Date 
2021-05-20
Full Text 
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Correspondence & Author Name 
Keyword 
Liaoning cashmere goat, fibroblast growth factor 5, signaling pathway, transcript
Volume 
Vol.48 No.5 (September 2021)
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Chiang Mai Journal of Science

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