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Berberine Loaded Nanoparticles Induce Apoptosis in Human Fibrosarcoma Cells
Paper Type  |
Contributed Paper |
Title |
Berberine Loaded Nanoparticles Induce Apoptosis in Human Fibrosarcoma Cells |
Author |
Minh Thi Hong Nguyen, Lam Luong Nguyen, Tae-Hee Kim, Won-Kyo Jung and Phuong Thi Mai Nguyen* |
Email |
phuongnguyenibt@gmail.com |
|
Abstract: Berberine, an isoquinoline alkaloid, is a target compound for anticancer effects against several cancer cells. However, the poor solubility of berberine in water could cause insufficient bioavailability.
In the present study, berberine loaded nanoparticles (nanoberberine) were synthesized to improve its
dispersion in water and consequently to increase its pharmacokinetic profile. The data showed that the
nanoberberine was successfully synthesized in spherical shape at nano size with approximately 60 nm
of mean size. Furthermore, cell viability assay was performed and showed that nanoberberine inhibited
proliferation of human fibrosarcoma cell in a dose-dependent manner and its effect is better than that
observed with berberine treatment. Nanoberberine treatment at a concentration of 20 μg/mL resulted
in only 33.5% viable cells compared to 53.6% of berberine treated samples. The effect of nanoberberine
and berberine on promoting apoptosis on HT1080 cells was determined through DNA staining and
Annexin V/ Propidium Iodide (PI) double staining. Our results indicated that nanoberberine treatment
induced chromatin condensation and loss of plasma membrane properties more pronounced than
those induced by berberine free form. These findings suggest that nanoberberine enhanced HT1080
cancer cells undergone apoptosis and improved its bioavailability compared to the original form. |
|
Start & End Page |
123 - 134 |
Received Date |
2020-04-10 |
Revised Date |
|
Accepted Date |
2020-07-07 |
Full Text |
Download |
File Supplemental |
Download Supplemental |
Keyword |
berberine, nanoberberine, apoptosis, fibrosarcoma cells, cytotoxicity |
Volume |
Vol.48 No.1 (January 2021) |
DOI |
|
Citation |
Nguyen M.T.H., Nguyen L.L., Kim T., Jung W. and Nguyen P.T.M., Berberine Loaded Nanoparticles Induce Apoptosis in Human Fibrosarcoma Cells, Chiang Mai J. Sci., 2021; 48(1): 123-134. |
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