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L-Asparaginase Production by Bipolaris sp. BR438 Isolated from Brown Rice in Thailand


Paper Type 
Short Communication
Title 
L-Asparaginase Production by Bipolaris sp. BR438 Isolated from Brown Rice in Thailand
Author 
Kodchakorn Lapmak, Saisamorn Lumyong, Sutheera Thongkuntha, Pairote Wongputtisin, and Uraporn Sardsu
Email 
k_lapmak@hotmail.com
Abstract:
Thirty-six filamentous fungal isolates obtained from brown rice in Thailand were screened for their ability to produce L-asparaginase. Using modified Czapek Dox (mCD) agar containing L-asparagine and phenol red as indicator, 24 L-asparaginase producing fungal isolates could be preliminary identified by observing pink colour formation. It was found that isolate BR438 exhibited highest activity (6.3 +/- 0.65 U/ml) when cultured in the mCD medium containing 1% L-asparagine and 0.4% glucose at 30oC for 72 h. Its asparaginase was also proved to be non-cytotoxic when tested against Vero cell lines. The identification based on internal transcribed spacer (ITS) regions of ribosomal DNA and morphological study showed that isolate BR438 was Bipolaris  sp. and was closely related to  Bipolaris australiensis and B. ovariicola.
Start & End Page 
160 - 164
Received Date 
2009-02-16
Revised Date 
Accepted Date 
2009-05-06
Full Text 
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Keyword 
Bipolaris, brown rice, L-asparaginase, cytotoxicity
Volume 
Vol.37 No.1 (JANUARY 2010)
DOI 
Citation 
Lapmak K., Lumyong S., Thongkuntha S., Wongputtisin P., and Sardsu U., L-Asparaginase Production by Bipolaris sp. BR438 Isolated from Brown Rice in Thailand, Chiang Mai Journal of Science, 2010; 37(1): 160-164.
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