Chiang Mai Journal of Science

Centotheca lappacea extracts were prepared by maceration with 95% (v/v) ethanol or decoction with distilled water. The extracts were tested for in-vitro anti-aging activities including DPPH radical scavenging, metal ion chelating, lipid peroxidation inhibition, tyrosinase inhibition, collagenase inhibition, gelatinolytic activity (MMP-2 inhibition activity) on human skin fibroblasts and estrogenic effect in human breast cancer cell line (MCF-7). The ethanolic extract exhibited better activities than the aqueous extract on DPPH scavenging, metal chelating and lipid peroxidation inhibition with the SC50, CC50 and IPC50 values of 1.39±0.50, 366.95±13.47 and 0.99±0.26 mg/ml, respectively, as well as mild tyrosinase inhibition activity (IC50 value of 3,756.71±169.14 mg/ml). This ethanolic extract also gave strong collagenase inhibition with the IC50 value of 1.26±1.01 mg/ml which was close to ascorbic acid (IC50 value of 1.24±0.25 mg/ml). For the MMP-2 inhibition, the extract gave the percentage of pro MMP-2 inhibition of 21.49±2.60% which was almost the same as that of ascorbic acid (24.19±1.60%). The extract at the concentration of 1000 mg/ml also showed estrogenic activity by increasing the MCF-7 cell proliferation of 107.05±17.36% of the control. The results from this study have suggested the development potential as an anti-aging product of the ethanolic extract from C. lappacea and the confirmation of the traditional use of C. lappacea for rejuvenile in women after laboring.