Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium

Sureewan Sittijunda and Alissara Reungsang
* Author for corresponding; e-mail address: sureewan.sit@mahidol.ac.th
Volume: Vol.46 No.1 (January 2019)
Research Article
DOI:
Received: 3 April 2018, Revised: -, Accepted: 19 June 2018, Published: -

Citation: Sittijunda S. and Reungsang A., Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium, Chiang Mai Journal of Science, 2019; 46(1): 1-14.

Abstract

Response surface methodology with Box-Behnken design was applied to identify optimum culture compositions for 1,3-propanediol (1,3-PD) production from crude glycerol by an anaerobic mixed microbial consortium. Endo nutrient addition strongly influenced 1,3-PD production with a maximum 1,3-PD concentration of 33.72 g/dm3 achieved at 60.18 g/dm3 crude glycerol, 2.73 g/dm3 yeast extract, 205.51 mg/dm3 Na2HPO4, and 0.60 cm3/dm3 Endo nutrient. A verification experiment determined maximum concentration of 1,3-PD and glycerol consumption at 30.30 ± 9.78 g/dm3 and 70.61%, respectively, confirming the validity of the predicted model. Microbial community analysis by polymerase chain reaction-denaturing gradient gel electrophoresis revealed predominant 1,3-PD producing bacteria in the fermentation broth at optimum condition as Enterobacter sp. and Citrobacter sp.

Keywords: PDO, biodiesel, glycerol waste, bioplastic, ethanol

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