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Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium


Paper Type 
 Contributed Paper
Title 
 Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium
Author 
 Sureewan Sittijunda and Alissara Reungsang
Email 
 sureewan.sit@mahidol.ac.th
Abstract:
Response surface methodology with Box-Behnken design was applied to identify optimum culture compositions for 1,3-propanediol (1,3-PD) production from crude glycerol by an anaerobic mixed microbial consortium. Endo nutrient addition strongly influenced 1,3-PD production with a maximum 1,3-PD concentration of 33.72 g/dm3 achieved at 60.18 g/dm3 crude glycerol, 2.73 g/dm3 yeast extract, 205.51 mg/dm3 Na2HPO4, and 0.60 cm3/dm3 Endo nutrient. A verification experiment determined maximum concentration of 1,3-PD and glycerol consumption at 30.30 ± 9.78 g/dm3 and 70.61%, respectively, confirming the validity of the predicted model. Microbial community analysis by polymerase chain reaction-denaturing gradient gel electrophoresis revealed predominant 1,3-PD producing bacteria in the fermentation broth at optimum condition as Enterobacter sp. and Citrobacter sp.
Start & End Page 
 1 - 14
Received Date 
 2018-04-03
Revised Date 
Accepted Date 
 2018-06-19
Full Text 
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Keyword 
 PDO, biodiesel, glycerol waste, bioplastic, ethanol
Volume 
 Vol.46 No.1 (January 2019)
DOI 
Citation 
 Sittijunda S. and Reungsang A., Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium, Chiang Mai Journal of Science, 2019; 46(1): 1-14.
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