Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium

Sureewan Sittijunda and Alissara Reungsang
* Author for corresponding; e-mail address: sureewan.sit@mahidol.ac.th
Volume: Vol.46 No.1 (January 2019)
Research Article
DOI:
Received: 3 April 2018, Revised: -, Accepted: 19 June 2018, Published: -

Citation: Sittijunda S. and Reungsang A., Bioconversion of Crude Glycerol into 1,3-propanediol by an Anaerobic Mixed Microbial Consortium, Chiang Mai Journal of Science, 2019; 46(1): 1-14.

Abstract

Response surface methodology with Box-Behnken design was applied to identify optimum culture compositions for 1,3-propanediol (1,3-PD) production from crude glycerol by an anaerobic mixed microbial consortium. Endo nutrient addition strongly influenced 1,3-PD production with a maximum 1,3-PD concentration of 33.72 g/dm3 achieved at 60.18 g/dm3 crude glycerol, 2.73 g/dm3 yeast extract, 205.51 mg/dm3 Na2HPO4, and 0.60 cm3/dm3 Endo nutrient. A verification experiment determined maximum concentration of 1,3-PD and glycerol consumption at 30.30 ± 9.78 g/dm3 and 70.61%, respectively, confirming the validity of the predicted model. Microbial community analysis by polymerase chain reaction-denaturing gradient gel electrophoresis revealed predominant 1,3-PD producing bacteria in the fermentation broth at optimum condition as Enterobacter sp. and Citrobacter sp.

Keywords: PDO, biodiesel, glycerol waste, bioplastic, ethanol

Growth and Lipid Accumulation in Isolated Microalgae Strains from Vietnamese Waterbodies for Biodiesel Production
DOI: 10.12982/CMJS.2025.076.

Le Anh Pham, Thi Oanh Doan, Lan Phuong Doan, Thi My Nguyen, Thi Anh Nguyet Nguyen, Phuong Thao Pham, Thi Quynh Hoang, Thi Phuong Quynh Le, Anh Thao Nguyen, Thi Thu Lien Nguyen, Anh Tu Tran and Thi Thuy Duong

Vol.52 No.5 (September 2025)
Research Article View: 567 Download: 137

Reinforcement of Polylactic acid (PLA) with Rice Straw Fibers for the Production of Bioplastics and their Characterization
DOI: 10.12982/CMJS.2024.077.

Juntima Chungsiriporn, Prukraya Pongyeela, Nirana Chairerk and Jutarut Iewkittayakorn

Vol.51 No.5 (September 2024)
Research Article View: 1,556 Download: 943

Optimizing Process Parameters through Response Surface Methodology for Transesterification Using an Innovative Extruded Catalyst
DOI: 10.12982/CMJS.2024.046.

Jutaporn Chanathaworn and Sommai Saramath

Vol.51 No.3 (May 2024)
Research Article View: 872 Download: 586

Biodiesel Production from Waste Cooking Oil using Heterogeneous CaO/Zn Catalyst: Yield and Reusability Performance
DOI: 10.12982/CMJS.2023.070.

Nur Afiqah Ali and Nozieana Khairuddin

Vol.50 No.6 (November 2023)
Research Article View: 1,133 Download: 1,147

Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
DOI: 10.12982/CMJS.2022.109.

Wisan Chuavong, Sirikwan Ponprateep, Pravech Ajawatanawong and Tipachai Vatanavicharn

Vol.49 No.6 (November 2022)
Research Article View: 1,807 Download: 793

Study the Stable Diatomite-supported Catalyst for Biodiesel Production
DOI: 10.12982/CMJS.2022.083.

Wilasinee Kingkam, Kewalee Nilgumhang, Sasikarn Nuchdang, Bheechanat Duangdee, Vichai Puripunyavanich and Dussadee Rattanaphra

Vol.49 No.5 (September 2022)
Research Article View: 1,423 Download: 838

Conversion of Palm Oil into Biodiesel Production with Heterogeneous Catalyst Derived from Spent Coffee Grounds Ash: Process Optimization Through Response Surface Methodology
page: 580 - 599

Jakkrapong Jitjamnong*, Natthida Numwong, Narinphop Chuaykarn, Chatrawee Direksilp, Apanee Luengnaruemitchai, Napaphat Kongrit and Nonlapan Khantikulanon

Vol.48 No.2 (March 2021)
Research Article View: 1,243 Download: 194

Effect of Calcination Temperature of Musa sapientum Linn Peels as a Novel Biobased Heterogeneous Catalyst for Soybean Oil-derived Fatty Acid Methyl Ester Formation
page: 484 - 498

Jakkrapong Jitjamnong, Chachchaya Thunyaratchatanon, Apanee Luengnaruemitchai, Napaphat Kongrit, Naparat Kasetsomboon, Narinphop Chuaykarn and Nonlapan Khantikulanon

Vol.47 No.3 (May 2020)
Research Article View: 1,132 Download: 744

Biodiesel Production from Canola Oil and Methanol Using Ba Impregnated Calcium Oxide with Microwave Irradiation-Assistance
page: 987 - 1000

Jakkrapong Jitjamnong*, Apanee Luengnaruemitchai, Napaphat Samanwonga and Narinphop Chuaykarn

Vol.46 No.5 (September 2019)
Research Article View: 1,062 Download: 456

Optimization of Culture Conditions for Oil Production by the Double Mutant of Chlorella sorokiniana DMKU5202-D223
page: 1055 - 1068

Mathurin Vichachucherd, Wichien Yongmanitchai and Duenrut Chonudomkul *

Vol.46 No.6 (November 2019)
Research Article View: 1,552 Download: 488

Optimization of Oleic Acid Esterification for Biodiesel Production using Brønsted Acidic Ionic Liquid as a Catalyst
page: 714 - 726

Benyong Han, Fang Yin*, Shiqing Liu, Xingling Zhao, Jing Liu, Changmei Wang, Hong Yang, Wudi Zhang*

Vol.46 No.4 (July 2019)
Research Article View: 1,061 Download: 636

Isolation of a High Potential Thermotolerant Strain of Zymomonas mobilis for Ethanol Production at High Temperature Using Ethyl Methane Sulfonate Mutagenesis
page: 207 - 218

Jatupat Samappito, Preekamol Klanrit, Sudarat Thanonkeo, Mamoru Yamada and Pornthap Thanonkeo

Vol.46 No.2 (March 2019)
Research Article View: 910 Download: 293

Enhanced Growth and Ethanol Oxidation by Overexpressed caiA Gene Encoding acyl-CoA Dehydrogenase in Komagataeibacter medellinensis NBRC 3288
page: 196 - 206

Phisurang Konjanda, Toshiharu Yakushi, Kazunobu Matsushita and Gunjana Theeragool

Vol.46 No.2 (March 2019)
Research Article View: 1,045 Download: 343

Enhancement of Biomass, Lipid and Hydrocarbon Production from Green Microalga, Botryococcus braunii AARL G037, by UV-C Induction
page: 2637 - 2651

Theera Thurakit, Chayakorn Pumas, Wasu Pathom-aree, Jeeraporn Pekkoh and Yuwadee Peerapornpisal

Vol.45 NO.7 (November 2018)
Research Article View: 1,614 Download: 403

Synthesis and Characterization of Biolubricant from POME Oil and Hepatopancreas Lipase from Pacific White Shrimp (Litopenaeus vannamei)
page: 2438 - 2453

Thanaphorn Rakkan, Nisa Paichid, Tewan Yunu, Sappasith Klomklao and Kanokphorn Sangkharak

Vol.45 No.6 (September 2018)
Research Article View: 1,564 Download: 435

Efficient Conversion of Oils to Biodiesel Catalyzed by Uncalcined Disodium Metasilicate Granules
page: 1888 - 1900

Wuttichai Roschat, Mattana Kacha, Boonyawan Yoosuk, Preecha Moonsin, Taweesak Sudyoadsuk and Vinich Promarak

Vol.45 No.4 (July 2018)
Research Article View: 1,075 Download: 292

Ethanol Sensors Fabricated from PdO/WO3 Nanorods
page: 1827 - 1834

Sathukarn Kabcum, Duangdao Channei, Adisorn Tuantranont, Anurat Wisitsiraat, Chaikarn Liewhiran and Sukon Phanichphant

Vol.45 No.4 (July 2018)
Research Article View: 1,023 Download: 291

1, 3-Propanediol Production from Crude Glycerol by Klebsiella pneumoniae
page: 718 - 725

Jiaxin Wu*[a], Fenghuan Wang[b], Zheng Wang[b], Hua Ye[c] and Peiyi Liu[d]

Vol.43 No.4 (JULY 2016)
Research Article View: 965 Download: 287

Assessment of genetic diversity of Jatropha curcas L. using AFLP and ISSR markers
page: 614 - 625

Sirisak Soonthornyatara, Prapa Sripichitt, Rugsarit Kaveeta and Vipa Hongtrakul

Vol.42 No.3 (JULY 2015)
Research Article View: 1,287 Download: 297

Morphology and Catalytic Activity of Gold Core-Platinum Shell Nanoparticles
page: 481 - 489

Kontee Thongthai, Laongnuan Srisombat, Surin Saipanya and Supon Ananta

Vol.42 No.2 (APRIL 2015)
Research Article View: 922 Download: 277

Effects of initial glucose concentration on fermentation performance of Kluyveromyces marxianus cells immobilized on banana leaf sheath pieces
page: 294 - 303

Hoang Du Le, Van Viet Man Le

Vol.42 No.2 (APRIL 2015)
Research Article View: 986 Download: 308

Improvement of continuous ethanol fermentation from sweet sorghum juice by Saccharomyces cerevisiae using stirred tank bioreactor coupling with plug flow bioreactor
page: 282 - 293

Naulchan Khongsay, Lakkana Laopaiboon and Pattana Laopaiboon

Vol.42 No.2 (APRIL 2015)
Research Article View: 942 Download: 262

β-Carotene Production by Sporobolomyces pararoseus TISTR5213 Using Crude Glycerol as the Sole Carbon Source
page: 17 - 33

Atchara Manowattana [a], Charin Techapun [b], Phisit Seesuriyachan [b], Prasert Hanmoungjai [b] and

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,007 Download: 239

Anti-inflammatory Activity of Ethanol Extract from the Leaves of Pseuderanthemum palatiferum (Nees) Radlk.
page: 321 - 331

Taddaow Khumpook [a], Siriwadee Chomdej [a, c], Supap Saenphet [a], Doungporn Amornlerdpison [b] and

Vol.40 No.3 (JULY 2013)
Research Article View: 964 Download: 319

Preparing Activated Carbon from Palm Shell for Biodiesel Fuel Production
page: 572 - 578

Achanai Buasri, Nattawut Chaiyut and Chaiwut Nakweang

Vol.38 No.4 (OCTOBER 2011)
Research Article View: 951 Download: 347

Biodiesel Synthesis from Transesterification by Clay-based Catalyst
page: 201 - 207

Manut Jaimasith and Satit Phiyanalinmat

Vol.34 No.2 (MAY 2007)
Research Article View: 1,003 Download: 287

Production Methyl Esters from Palm Fatty Acids in Supercritical Methanol
page: 23 - 28

Duangkamol Yujaroen, Artiwan Shotipruk, Motonobu Goto and Mitsuru Sasaki

Vol.35 No.1 (JANUARY 2008)
Research Article View: 1,048 Download: 292

Chiang Mai Journal of Science

1,647

Q3 0.80

Q3 1.3

7 days

Outline

Figures and Tables