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Co-production of Hydrogen and Ethanol of Escherichia coli SS1 Isolate


Paper Type 
Contributed Paper
Title 
Co-production of Hydrogen and Ethanol of Escherichia coli SS1 Isolate
Author 
Chiu-Shyan Soo, Wai-Sum Yap, Wei-Min Hon, Norhayati Ramli, Umi Kalsom Md Shah and Lai-Yee Phang*
Email 
phanglaiyee@upm.edu.my
Abstract:
Simultaneous production of hydrogen and ethanol from waste materials has potential for the development of a more cost-effective biofuels generation process. This study aimed to conduct glycerol fermentation using Escherichia coli SS1 to establish its hydrogen and ethanol co-production profile. Anaerobic fermentation was performed at 37°C with different concentrations of glycerol as a substrate. E. coli SS1 had exponential growth within 24 h (OD600 of 1.6), and hydrogen and ethanol were produced in abundance within 48 h of fermentation. Fermentation using 10 g/l of glycerol achieved the highest yield, 0.57 mol of hydrogen and 0.88 mol of ethanol per mol of glycerol. The highest hydrogen productivity (1.85 mmol/l/h) and ethanol productivity (3.13 mmol/l/h) were obtained at 45 g/l of glycerol. This report provides the complete data set for hydrogen and ethanol co-production yield and productivity by the wild-type E. coli SS1 and serves as a useful reference for other researchers working on the co-production of hydrogen and ethanol.
Start & End Page 
768 - 773
Received Date 
2015-12-03
Revised Date 
Accepted Date 
2016-10-06
Full Text 
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Keyword 
hydrogen, ethanol, co-production, Escherichia coli, glycerol
Volume 
Vol.44 No.3 (July 2017)
DOI 
Citation 
Soo C., Yap W., Hon W., Ramli N., Shah U.K.M. and Phang* L., Co-production of Hydrogen and Ethanol of Escherichia coli SS1 Isolate, Chiang Mai Journal of Science, 2017; 44(3): 768-773.
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