Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Co-production of Hydrogen and Ethanol of Escherichia coli SS1 Isolate

Chiu-Shyan Soo, Wai-Sum Yap, Wei-Min Hon, Norhayati Ramli, Umi Kalsom Md Shah and Lai-Yee Phang*
* Author for corresponding; e-mail address: phanglaiyee@upm.edu.my
Volume: Vol.44 No.3 (July 2017)
Research Article
DOI:
Received: 3 December 2015, Revised: -, Accepted: 6 October 2016, Published: -

Citation: Soo C., Yap W., Hon W., Ramli N., Shah U.K.M. and Phang L., Co-production of Hydrogen and Ethanol of Escherichia coli SS1 Isolate, Chiang Mai Journal of Science, 2017; 44(3): 768-773.

Abstract

Simultaneous production of hydrogen and ethanol from waste materials has potential for the development of a more cost-effective biofuels generation process. This study aimed to conduct glycerol fermentation using Escherichia coli SS1 to establish its hydrogen and ethanol co-production profile. Anaerobic fermentation was performed at 37°C with different concentrations of glycerol as a substrate. E. coli SS1 had exponential growth within 24 h (OD600 of 1.6), and hydrogen and ethanol were produced in abundance within 48 h of fermentation. Fermentation using 10 g/l of glycerol achieved the highest yield, 0.57 mol of hydrogen and 0.88 mol of ethanol per mol of glycerol. The highest hydrogen productivity (1.85 mmol/l/h) and ethanol productivity (3.13 mmol/l/h) were obtained at 45 g/l of glycerol. This report provides the complete data set for hydrogen and ethanol co-production yield and productivity by the wild-type E. coli SS1 and serves as a useful reference for other researchers working on the co-production of hydrogen and ethanol.

Keywords: hydrogen, ethanol, co-production, Escherichia coli, glycerol

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