Chiang Mai Journal of Science

Characteristics of D-lactate Dehydrogenase from the High Potential D-lactic Acid Producer Leuconostoc pseudomesenteroides TC49 Isolated from Thailand

Paper Type	Contributed Paper
Title	Characteristics of D-lactate Dehydrogenase from the High Potential D-lactic Acid Producer Leuconostoc pseudomesenteroides TC49 Isolated from Thailand
Author	Tasneem Chemama, Junji Hayashi, Mamoru Wakayama* and Narumol Thongwai*
Email	nthongw@hotmail.com, wakayama@sk.ritsumei.ac.jp
Abstract:  D-lactate dehydrogenase obtained from Leuconostoc pseudomesenteroides TC49, a D-lactic acid producing bacterium isolated from a Tithonia diversifolia flower in Thailand, was studied its properties for use in D-lactic acid production. Successful protocols of protein precipitation, dialysis, ultrafiltration and chromatography were used for D-LDH purification. The purified D-LDH with its N-terminal amino acid sequence as MKIFAYGIRE displayed the molecular weight of approximate 40.6 kDa with preferred pH of 8.5 and temperature of 30°C for its highest activity. The enzyme stability was decreased with increasing temperature and was completely vanished at 50°C. The kcat, Km and kcat/ Km of the purified enzyme in the pyruvate reduction were 180 s-1, 0.5 mM and 360 mM-1 s-1 while the values in the D-lactate oxidation were 117 s-1, 69.6 mM and 1.681 mM-1s-1, respectively. AgNO3 and ZnCl2 slightly inhibited the purified enzyme.
Start & End Page	42 - 55
Received Date	2020-03-02
Revised Date
Accepted Date	2020-07-09
Full Text	Download
Keyword	Leuconostoc pseudomesenteroides, D-lactate dehydrogenase, D-lactic acid, N-terminal amino aid sequence, pyruvic acid, native-PAGE
Volume	Vol.48 No.1 (January 2021)
DOI
Citation	Chemama T., Hayashi J., Wakayama M. and Thongwai N., Characteristics of D-lactate Dehydrogenase from the High Potential D-lactic Acid Producer Leuconostoc pseudomesenteroides TC49 Isolated from Thailand, Chiang Mai J. Sci., 2021; 48(1): 42-55.
SDGs
View:691 Download:495