Chiang Mai Journal of Science

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Carica papaya lipase (CPL) revealed only 10.8% methyl ester content from methanolysis of triolein in a solvent-free reaction system. Using CPL washing with iso-propanol (iPCPL), the methyl ester content was increased to 63.6%. The incorporation of non-polar organic solvents to the methanolysis of triolein catalyzed by iPCPL did not influence the formation of methyl ester. The highest content of methyl ester (69.8%) was obtained from the methanolysis using a 1/4 molar ratio of triolein/dried methanol in a solvent-free system. An addition of 5-20% (v/v) water to the reaction mixture slightly decreased the yield of methyl ester after 24 h of reaction time.

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