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Improvement of Carica papaya Lipase for Methanolysis of Triolein


Paper Type 
Contributed Paper
Title 
Improvement of Carica papaya Lipase for Methanolysis of Triolein
Author 
Porntippa Angkanurukpun [a], Pensiri Sriburi [b] and Pawinee Kanasawud*[b]
Email 
scipknsw@chiangmai.ac.th
Abstract:
Carica papaya lipase (CPL) revealed only 10.8% methyl ester content from methanolysis of triolein in a solvent-free reaction system. Using CPL washing with iso-propanol (iPCPL), the methyl ester content was increased to 63.6%. The incorporation of non-polar organic solvents to the methanolysis of triolein catalyzed by iPCPL did not influence the formation of methyl ester. The highest content of methyl ester (69.8%) was obtained from the methanolysis using a 1/4 molar ratio of triolein/dried methanol in a solvent-free system. An addition of 5-20% (v/v) water to the reaction mixture slightly decreased the yield of methyl ester after 24 h of reaction time.
Start & End Page 
217 - 222
Received Date 
2005-06-24
Revised Date 
Accepted Date 
2006-12-02
Full Text 
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Keyword 
Carica papaya lipase, iso-propanol washing, methanolysis, triolein
Volume 
Vol.33 No.2 (MAY 2006)
DOI 
Citation 
[a] P.A., [b] P.S. and Kanasawud*[b] P., Improvement of Carica papaya Lipase for Methanolysis of Triolein, Chiang Mai Journal of Science, 2006; 33(2): 217-222.
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