Improvement of Carica papaya Lipase for Methanolysis of Triolein
Porntippa Angkanurukpun [a], Pensiri Sriburi [b] and Pawinee Kanasawud*[b]* Author for corresponding; e-mail address: scipknsw@chiangmai.ac.th
Volume: Vol.33 No.2 (MAY 2006)
Research Article
DOI:
Received: 24 June 2005, Revised: -, Accepted: 2 December 2006, Published: -
Citation: Angkanurukpun P., Sriburi P. and Kanasawud P., Improvement of Carica papaya Lipase for Methanolysis of Triolein, Chiang Mai Journal of Science, 2006; 33(2): 217-222.
Abstract
Carica papaya lipase (CPL) revealed only 10.8% methyl ester content from methanolysis of triolein in a solvent-free reaction system. Using CPL washing with iso-propanol (iPCPL), the methyl ester content was increased to 63.6%. The incorporation of non-polar organic solvents to the methanolysis of triolein catalyzed by iPCPL did not influence the formation of methyl ester. The highest content of methyl ester (69.8%) was obtained from the methanolysis using a 1/4 molar ratio of triolein/dried methanol in a solvent-free system. An addition of 5-20% (v/v) water to the reaction mixture slightly decreased the yield of methyl ester after 24 h of reaction time.