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Biohydrogen Production by Extremely Halophilic Bacteria from the Salt Pan of Samut Sakhon, Thailand


Paper Type 
Contributed Paper
Title 
Biohydrogen Production by Extremely Halophilic Bacteria from the Salt Pan of Samut Sakhon, Thailand
Author 
Dyah Asri Handayani Taroepratjeka, Tsuyoshi Imai, Prapaipid Chairattanamanokorn and Alissara Reungsang
Email 
imai@yamaguchi-u.ac.jp
Abstract:
 The optimum conditions for biohydrogen production from glucose with extremely halophilic
bacteria from Samut Sakhon salt pan, Thailand were identified. The extremely halophilic bacteria’s
ability to produce hydrogen in nearly saturated NaCl concentration provides a potential benefit of
cutting the cost of water and sterilization in dark fermentation process following alkaline pretreatment
of lignocellulosic biomass. A hydrogen molar yield of 1.45 mol H2/mol glucose at optimum conditions
of 26% NaCl, 35°C, and pH 9 was obtained. The acclimatized bacteria were able to ferment various
types of lignocellulosic-derived sugars. Through PCR-DGGE, the predominant hydrogen producer
among the mixed culture was found to be Halanaerobium fermentans. These findings provided insights
into the application of extremely halophilic bacteria for biohydrogen production.
 
Start & End Page 
378 - 390
Received Date 
2019-07-05
Revised Date 
Accepted Date 
2019-10-08
Full Text 
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Keyword 
biohydrogen, extremely halophilic bacteria, Halanaerobium fermentans, dark fermentation
Volume 
Vol.47 No.3 (May 2020)
DOI 
Citation 
Taroepratjeka D.A.H., Imai T. and Reungsang P.C.A., Biohydrogen Production by Extremely Halophilic Bacteria from the Salt Pan of Samut Sakhon, Thailand, Chiang Mai J. Sci., 2020; 47(3): 378-390.
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