Chiang Mai Journal of Science

Biohydrogen Production by Extremely Halophilic Bacteria from the Salt Pan of Samut Sakhon, Thailand

Paper Type	Contributed Paper
Title	Biohydrogen Production by Extremely Halophilic Bacteria from the Salt Pan of Samut Sakhon, Thailand
Author	Dyah Asri Handayani Taroepratjeka, Tsuyoshi Imai, Prapaipid Chairattanamanokorn and Alissara Reungsang
Email	imai@yamaguchi-u.ac.jp
Abstract:  The optimum conditions for biohydrogen production from glucose with extremely halophilic bacteria from Samut Sakhon salt pan, Thailand were identified. The extremely halophilic bacteria’s ability to produce hydrogen in nearly saturated NaCl concentration provides a potential benefit of cutting the cost of water and sterilization in dark fermentation process following alkaline pretreatment of lignocellulosic biomass. A hydrogen molar yield of 1.45 mol H2/mol glucose at optimum conditions of 26% NaCl, 35°C, and pH 9 was obtained. The acclimatized bacteria were able to ferment various types of lignocellulosic-derived sugars. Through PCR-DGGE, the predominant hydrogen producer among the mixed culture was found to be Halanaerobium fermentans. These findings provided insights into the application of extremely halophilic bacteria for biohydrogen production.
Start & End Page	378 - 390
Received Date	2019-07-05
Revised Date
Accepted Date	2019-10-08
Full Text	Download
Keyword	biohydrogen, extremely halophilic bacteria, Halanaerobium fermentans, dark fermentation
Volume	Vol.47 No.3 (May 2020)
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