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Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes


Paper Type 
Contributed Paper
Title 
Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes
Author 
Xiaosheng Tang, Ping Tang and Liangliang Liu
Email 
liuliangliang@caas.cn
Abstract:
Carbon nanotube (CNT) has been used for the immobilization of proteins and enzymes. However, the leakage of CNT into solution and difficulty in separation hindered the application of CNT. To minimize these shortcomings, CNT functionalized with magnetic particles was prepared and intended to be used in the immobilization of alcohol dehydrogenase (ADH) in this study. The obtained magnetic CNT (MCNT) was characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and vibration sample magnetometer (VSM). The operational stability was evaluated in repeated assay process, and the MCNT immobilized ADH (MCNT-ADH) retained 87.6 % of the initial activity after being used for 10 times. Compared with free ADH, MCNT-ADH exhibited improved pH and thermal stabilities. As a kind of novel immobilization support, MCNT could find considerable potential uses in industrial processes due to its better performance.
Start & End Page 
211 - 219
Received Date 
2017-02-28
Revised Date 
Accepted Date 
2017-05-04
Full Text 
  Download
Keyword 
alcohol dehydrogenase, carbon nanotube, immobilization, magnetic nanoparticle
Volume 
Vol.45 No.1 (January 2018)
DOI 
Citation 
Tang X., Tang P. and Liu L., Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes, Chiang Mai J. Sci., 2018; 45(1): 211-219.
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Chiang Mai Journal of Science

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