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Enhanced Growth and Ethanol Oxidation by Overexpressed caiA Gene Encoding acyl-CoA Dehydrogenase in Komagataeibacter medellinensis NBRC 3288


Paper Type 
Contributed Paper
Title 
Enhanced Growth and Ethanol Oxidation by Overexpressed caiA Gene Encoding acyl-CoA Dehydrogenase in Komagataeibacter medellinensis NBRC 3288
Author 
Phisurang Konjanda, Toshiharu Yakushi, Kazunobu Matsushita and Gunjana Theeragool
Email 
fscignt@ku.ac.th
Abstract:
 In this study, the caiA gene encoding acyl-CoA dehydrogenase (ACAD) from
thermotolerant Komagataeibacter intermedius MSKU 9 was cloned and characterized. Two specific
primers for amplification of an entire caiA gene were designed from a draft genome sequence
of K. intermedius MSKU 9 (MSKU 9_0337 homolog). Sequencing analysis of the 1.2 kb PCR
product revealed possible open reading frame (ORF) of 1,182 bp with a start codon ATG at
the 1,001st - 1,003rd position and a stop codon TAA at the 2,183th - 2,185th position. The deduced
amino acid sequences of the caiA gene had 393 residues. The overexpression of the caiA gene
in a non-thermotolerant strain, K. medellinensis NBRC 3288, was investigated. K. medellinensis
NBRC 3288 harboring pMVcaiA showed a better growth rate than that observed in the parental
strain at 33°C and was more tolerant to both ethanol at 30°C and acetic acid even at 37°C.
Thus, it can be suggested that the caiA gene influenced and improved growth, acetic acid
tolerance and ethanol oxidation in acetic acid bacteria at high temperature.
Start & End Page 
196 - 206
Received Date 
2018-10-30
Revised Date 
Accepted Date 
2018-12-14
Full Text 
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Keyword 
Komagataeibacter, caiA gene, acyl-CoA dehydrogenase, acetic acid tolerance, ethanol oxidation
Volume 
Vol.46 No.2 (March 2019)
DOI 
Citation 
Konjanda P., Yakushi T., Matsushita K. and Theeragool G., Enhanced Growth and Ethanol Oxidation by Overexpressed caiA Gene Encoding acyl-CoA Dehydrogenase in Komagataeibacter medellinensis NBRC 3288, Chiang Mai J. Sci., 2019; 46(2): 196-206.
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