Paper Type |
Contributed Paper |
Title |
Enhanced Growth and Ethanol Oxidation by Overexpressed caiA Gene Encoding acyl-CoA Dehydrogenase in Komagataeibacter medellinensis NBRC 3288 |
Author |
Phisurang Konjanda, Toshiharu Yakushi, Kazunobu Matsushita and Gunjana Theeragool |
Email |
fscignt@ku.ac.th |
Abstract: In this study, the caiA gene encoding acyl-CoA dehydrogenase (ACAD) from
thermotolerant Komagataeibacter intermedius MSKU 9 was cloned and characterized. Two specific
primers for amplification of an entire caiA gene were designed from a draft genome sequence
of K. intermedius MSKU 9 (MSKU 9_0337 homolog). Sequencing analysis of the 1.2 kb PCR
product revealed possible open reading frame (ORF) of 1,182 bp with a start codon ATG at
the 1,001st - 1,003rd position and a stop codon TAA at the 2,183th - 2,185th position. The deduced
amino acid sequences of the caiA gene had 393 residues. The overexpression of the caiA gene
in a non-thermotolerant strain, K. medellinensis NBRC 3288, was investigated. K. medellinensis
NBRC 3288 harboring pMVcaiA showed a better growth rate than that observed in the parental
strain at 33°C and was more tolerant to both ethanol at 30°C and acetic acid even at 37°C.
Thus, it can be suggested that the caiA gene influenced and improved growth, acetic acid
tolerance and ethanol oxidation in acetic acid bacteria at high temperature. |
|
Start & End Page |
196 - 206 |
Received Date |
2018-10-30 |
Revised Date |
|
Accepted Date |
2018-12-14 |
Full Text |
Download |
Keyword |
Komagataeibacter, caiA gene, acyl-CoA dehydrogenase, acetic acid tolerance, ethanol oxidation |
Volume |
Vol.46 No.2 (March 2019) |
DOI |
|
Citation |
Konjanda P., Yakushi T., Matsushita K. and Theeragool G., Enhanced Growth and Ethanol Oxidation by Overexpressed caiA Gene Encoding acyl-CoA Dehydrogenase in Komagataeibacter medellinensis NBRC 3288, Chiang Mai J. Sci., 2019; 46(2): 196-206. |
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