Chiang Mai Journal of Science

Isolation of a High Potential Thermotolerant Strain of Zymomonas mobilis for Ethanol Production at High Temperature Using Ethyl Methane Sulfonate Mutagenesis

Paper Type	Contributed Paper
Title	Isolation of a High Potential Thermotolerant Strain of Zymomonas mobilis for Ethanol Production at High Temperature Using Ethyl Methane Sulfonate Mutagenesis
Author	Jatupat Samappito, Preekamol Klanrit, Sudarat Thanonkeo, Mamoru Yamada and Pornthap Thanonkeo
Email	portha@kku.ac.th
Abstract:  The wild-type strain Zymomonas mobilis TISTR548 has a critical temperature for its growth and ethanol production at 38 C. To obtain a high potential thermotolerant strain of this ethanologenic bacterium, this wild-type strain was subjected to chemical mutagenesis using ethyl methane sulfonate (EMS). After EMS treatment, the mutagenized cells were screened at temperatures ranging from 38 to 40 C. The results showed that only six mutant strains, designated ZM EMS-52, ZM EMS-121, ZM EMS-143, ZM EMS-229, ZM EMS-263 and ZM EMS-297, could grow and produce a relatively high level of ethanol at 40 C. Among these mutants, ZM EMS-229 displayed higher growth and ethanol production capacity than did the wild-type and other mutants at 37 and 40 C. This mutant strain also showed greater resistance to high concentrations of ethanol, acetic acid and H2O2 at high temperature than did the wild-type. These findings suggested that the ZM EMS-229 was a good candidate for high-temperature ethanol fermentation.
Start & End Page	207 - 218
Received Date	2018-09-15
Revised Date
Accepted Date	2018-11-16
Full Text	Download
Keyword	ethanol production, ethyl methane sulfonate (EMS), mutagenesis, thermotolerant microorganism, Zymomonas mobilis
Volume	Vol.46 No.2 (March 2019)
DOI
Citation	Samappito J., Klanrit P., Thanonkeo S., Yamada M. and Thanonkeo P., Isolation of a High Potential Thermotolerant Strain of Zymomonas mobilis for Ethanol Production at High Temperature Using Ethyl Methane Sulfonate Mutagenesis, Chiang Mai J. Sci., 2019; 46(2): 207-218.
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