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Enhanced Production of Phytase, A Feed Enzyme, from Pichia kudriavzevii using Mutagenesis and Improved Culture Conditions
Paper Type  |
Contributed Paper |
Title |
Enhanced Production of Phytase, A Feed Enzyme, from Pichia kudriavzevii using Mutagenesis and Improved Culture Conditions |
Author |
Wanatchaporn Boontham, Natsuda Srivanichpoom, Pumin Nutaratat, Savitree Limtong and Nantana Srisuk |
Email |
fscints@ku.ac.th |
|
Abstract: Pichia kudriavzevii WB17-1, a phytase-producing yeast isolated from duck excrement, was found to produce both cell-bound and extracellular phytases. To enhance extracellular phytase, P. kudriavzevii WB17-1 was subjected to induced mutation. Ethylmethane sulfonate (EMS)-induced mutation resulted in 2,400 mutants. The mutant P. kudriavzevii WB17-1 EMS3 showed the highest extracellular phytase activity. This mutant possessed a 6.2-fold increase in enzyme activity compared to the wild type level. The wild type and mutant were subjected to characterization of PHYPk, a gene encoding P. kudriavzevii phytase. An open reading frame of 1,071 bp encoding 357 amino acids with a predicted protein molecular mass of 40.056 kDa was identified. To optimize the extracellular phytase activity of P. kudriavzevii WB17-1 EMS3, a response surface methodology (RSM) was employed. The highest extracellular phytase activity was obtained in the medium containing 2.95% dextrose and 0.58% peptone with an initial pH of 5.8. The optimized phytase activity was 2.3 times the level obtained under un-optimized conditions and was 14.4 times the wild type level. The results obtained here show successful yeast strain improvement and optimization of extracellular phytase by yeast using RSM.
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Start & End Page |
431 - 443 |
Received Date |
2018-09-04 |
Revised Date |
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Accepted Date |
2019-02-18 |
Full Text |
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File Supplemental |
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Keyword |
phytase, optimisation, mutagenesis, Pichia kudriavzevii, yeast |
Volume |
Vol.46 No.3 (May 2019) |
DOI |
|
Citation |
Boontham W., Srivanichpoom N., Nutaratat P., Limtong S. and Srisuk N., Enhanced Production of Phytase, A Feed Enzyme, from Pichia kudriavzevii using Mutagenesis and Improved Culture Conditions, Chiang Mai Journal of Science, 2019; 46(3): 431-443. |
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