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Suitable Growth Conditions and Nutrition Factors on In Vitro Culture of Phlebopus portentosus (Boletales)


Paper Type 
Short Communication
Title 
Suitable Growth Conditions and Nutrition Factors on In Vitro Culture of Phlebopus portentosus (Boletales)
Author 
Jaturong Kumla, Eric Danell, Boonsom Bussaban, and Saisamorn Lumyong
Email 
scboi009@chiangmai.ac.th
Abstract:
Phlebopus portentosus is one of the most popular wild edible mushrooms in northern Thailand. As this mushroom may not be obligately ectomycorrhizal, we look into the possibilities for its commercial cultivation. In this paper we report the effects of pH, temperature, carbon and nitrogen sources and 15 culture growth media on the growth of  P. portentosus. The results indicated that mycelia of P. portentosus grow well at pH 4.0. The fungus was able to grow at temperatures ranging from 20-35oC  , with an optimal growth temperature of 30oC. Glucose and ammonium salts produced the largest radial growth. Among 15 culture media tested, modified Murashige & Skoog and fungal-host media were the best for the mycelia growth. Sclerotia-like structures were formed on these media after three weeks of  incubation.
Start & End Page 
156 - 159
Received Date 
2010-03-26
Revised Date 
Accepted Date 
2010-05-06
Full Text 
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Keyword 
Ectomycorrhiza, Bolete, Phlebopus portentosus, in vitro culture.
Volume 
Vol.38 No.1 (JANUARY 2011)
DOI 
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Chiang Mai Journal of Science

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