Fluid Flow Induced Shear Stress Affects Cell Growth and Total Flavone Production by Phellinus igniarius in Stirred-Tank Bioreactor
Hu Zhu, Wei Liu, Baozhen Tian and Shuaisshuai Zhang* Author for corresponding; e-mail address: hzhu@live.cn
Volume: Vol.39 No.1 (JANUARY 2012)
Research Article
DOI:
Received: 8 October 2011, Revised: -, Accepted: 15 November 2011, Published: -
Citation: Zhu H., Liu W., Tian B. and Zhang S., Fluid Flow Induced Shear Stress Affects Cell Growth and Total Flavone Production by Phellinus igniarius in Stirred-Tank Bioreactor, Chiang Mai Journal of Science, 2012; 39(1): 69-75.
Abstract
The effect of fluid flow induced shear stress on dry cell mass and total flavone production in the cultivation of Phellinus igniarius has been studied. The results showed that at impeller tip speeds (ITS) of 0.628, 1.256 and 1.884 m·s-1, the maximal production of total flavone was 2.80, 3.17 and 2.19 g·L-1 respectively. A relatively high cell concentration of 7.8 g·L-1 by dry mycelial biomass was obtained in the bioreactor at an ITS of 1.256 m·s-1 . At these ITSs, the maximal apparent viscosity was 1.123, 1.814 and 1.465 mPa·s and the exopolysaccharide production reached to 7.2, 9.4 and 8.1 g·L-1 respectively. The flow behavior of fermented broths was recorded photographically during submerged culture. The information obtained should assist progress towards the large scale production of flavone by submerged cultivation of Phellinus igniarius.