Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

1,647
Articles
Q3 0.80
Impact Factor
Q3 1.3
CiteScore
7 days
Avg. First Decision
Outline
Figures and Tables
ARTICLE METRICS
Views 935
Downloads 295

β-glucosidase Producing Bacillus Isolated from Thua-nao, an Indigenous Fermented Soybean Food in Thailand

Nutwara Meannui, Siriporn Riebroy*, Pussadee Tangwatcharin, Jeong Hwa Hong, Punnanee Sumpavapol and
* Author for corresponding; e-mail address: agrsrpr@ku.ac.th
Volume: Vol.44 No.4 (October 2017)
Research Article
DOI:
Received: 17 August 2015, Revised: -, Accepted: 4 January 2016, Published: -

Citation: Meannui N., Riebroy S., Tangwatcharin P., Hong J.H. and And P.S., β-glucosidase Producing Bacillus Isolated from Thua-nao, an Indigenous Fermented Soybean Food in Thailand, Chiang Mai Journal of Science, 2017; 44(4): 1257-1269.

Abstract

The characteristics of commercial dried flatten Thua-nao collected from Northern of Thailand, namely oven dried (LPO) and sun dried (LPS) samples from Lamphun, sun dried sample from Chiang Mai (CMS) and sun dried sample from Chiang Rai (CRS),were investigated. The variations of chemical and physical characteristics were found among these Thua-nao products. Seventeen isolates of spore-forming bacteria were obtained from such products. Among these, four isolates (LPO-2, LPO-4, LPS-2 and CMS-1) were identified as Bacillus subtilis group by API 50CHB kit. For cellulolytic enzyme production, CMS-1 exhibited the highest clear zone on tryptic soy agar supplemented with carboxymethyl cellulose (P<0.05). Contrastingly, LPO-2 had the highest b-glucosidase activity with the lowest clear zone of cellulolytic enzyme activity (P<0.05). From 16S rRNA gene sequencing result, LPO-2 could be identified as Bacillus sp. and closely related to B. subtilis group.

Keywords: Bacillus, β-glucosidase, Thua-nao, fermented soybean

Related Articles

Screening of Lactic Acid Bacteria with Functional Enzyme Activities and GABA Production for Fermentation of Soy-Based Yogurt Alternatives
DOI: 10.12982/CMJS.2025.088.

Yi-Ting Chang, Yen‑Po Chen, I-Hsiang Hsueh and Jui-Yu Chou

Vol.52 No.6 (November 2025)
Research Article View: 296 Download: 149
Synergistic Effects of Plant Growth-Promoting Microorganisms on Growth and Development of Terap (Artocarpus odoratissimus Blanco) Seedlings
DOI: 10.12982/CMJS.2023.071.

Goh Ei Ping, Shiamala Devi Ramaiya and Muta Harah Zakaria

Vol.50 No.6 (November 2023)
Research Article View: 1,448 Download: 666
Occurrence, Identification and Preliminary Biological Control of Bulb Rot of Onion (Allium cepa)
DOI: 10.12982/CMJS.2023.031.

Kanchana Srimai and Angsana Akarapisan

Vol.50 No.3 (May 2023)
Research Article View: 1,626 Download: 695
Biosurfactant-Producing Bacteria and Microbial Analysis in Mae Soon Reservoir, Fang Oil Field, Chiang Mai, Thailand
DOI: 10.12982/CMJS.2023.024.

Ladapa Kumdhitiahutsawakul, Thanachai Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Yingmanee Tragoolpua and Sakunnee Bovonsombut

Vol.50 No.3 (May 2023)
Research Article View: 1,827 Download: 607
Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
DOI: 10.12982/CMJS.2022.109.

Wisan Chuavong, Sirikwan Ponprateep, Pravech Ajawatanawong and Tipachai Vatanavicharn

Vol.49 No.6 (November 2022)
Research Article View: 1,741 Download: 793
Correlation of Antifungal Activities and Nonribosomal Peptide Synthetase Gene Expression of Bacillus siamensis 1021
DOI: 10.12982/CMJS.2022.041.

Warinya Nuwong and Chokchai Kittiwongwattana

Vol.49 No.2 (March 2022)
Research Article View: 1,878 Download: 881
Heterologous Expression and Activity Analysis of the Collagenase Gene from Bacillus cereus MBL13
page: 1463 - 1477

Lili Liu, Jingfang Guo, Yue Ding, Haoyang Wang and Weiwei Cheng

Vol.48 No.6 (November 2021)
Research Article View: 1,162 Download: 539
Characterization and Evaluated Storage Viability of Strain, Bacillus velezensis PGA106 for Growth Promotion and Biocontrol of Fusarium Wilt in Tomato
page: 1 - 12

Sirirat Siripornvisal, Thanasak Lomthong, Nantana Srisuk, Shinji Tokuyama and Vichien Kitpreechavanich*

Vol.48 No.1 (January 2021)
Research Article View: 1,282 Download: 1,186
Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates
page: 943 - 957

Lili Liu, Ke Chen, Mengjun Zhang, Shengjuan Shi and Weiwei Cheng

Vol.47 No.5 (September 2020)
Research Article View: 1,501 Download: 713
L-lactic Acid Production by Lactobacillus salivarius L105 in Optimized Medium and Effects of Sugar Concentration
page: 887 - 898

Nitaya Boontim, Kridsada Unban, Wasu Pathom-aree, Piyanuch Niamsup, Chartchai Khanongnuch and Saisamorn Lumyong

Vol.47 No.5 (September 2020)
Research Article View: 1,176 Download: 920
Purification and Characterization of an Extracellular Chitinase from Bacillus thuringiensis R 176 using Solid State Fermentation with Shrimp Shells Waste
page: 495 - 509

Mathurot Chaiharn, Abhinya Pikomol and Saisamorn Lumyoung

Vol.46 No.3 (May 2019)
Research Article View: 921 Download: 450
Isolation of A Novel Collagenase-producing Strain from Animal Bone Wastes and Optimization of Its Enzyme Production
page: 219 - 235

Lili Liu*, Yuanyuan Meng, Xiaoning Dai and Ke Chen

Vol.46 No.2 (March 2019)
Research Article View: 996 Download: 327
Optimization of Succinic Acid Production by Actinobacillus sp. NP9-aA7 using Plackett-Burman Design Coupled with Box-Behnken Design
page: 1649 - 1666

Sukanya Phuengjayaem,Somboon Tanasupawat and Siriluk Teeradakorn

Vol.45 No.4 (July 2018)
Research Article View: 916 Download: 545
Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae
page: 1202 - 1213

Sasiprapa Krongdang, Jeffery S. Pettis, Geoffrey R. Williams and Panuwan Chantawannakul*

Vol.45 No.3 (May 2018)
Research Article View: 1,023 Download: 336
Structural Characterization and Mode of Action Studies on Salvicin K and Antimicrobial Peptide-like Bacteriocin b Peptide Isolated from Lactobacillus salivarius K4
page: 731 - 745

Kannika Thongkhao, Frank D. Snnichsen, Sunee Nitisinprasert and Kiattawee Choowongkomon*

Vol.45 No.2 (March 2018)
Research Article View: 911 Download: 295
Comparison and Characterization of Purified Cellulase and Xylanase from Bacillus amyloliquefaciens CX1 and Bacillus subtilis B4
page: 92 - 105

Kalawong Ratchara, Wakayama Mamoru, Anuntalabhochai Somboon, Wongsawad Chalobol and Sangwijit Kant

Vol.45 No.1 (January 2018)
Research Article View: 913 Download: 274
Antibiotics Constituents of Endophytic Bacillus amyloliquefaciens UD25 Extracted from a Medicinal Plant, Memecylon edule Roxb.
page: 788 - 799

Artidtaya Bhoonobtong, Sirirath Sodngam, Sophon Boonlue, Wandee Bunyatratchata and Wiyada Mongkolt

Vol.44 No.3 (July 2017)
Research Article View: 2,183 Download: 1,044
Optimization of Mixed Bacillus Cultures as An Inoculant in Northern Thai Style Fermented Soybeans (Thua-nao) by Mixture Design
page: 414 - 426

Patcharin Phokasem [a], Punpong Lekhakula [a], Niramon Utama-ung [b], Pornchai Rachtanapun [c] and P

Vol.44 No.2 (April 2017)
Research Article View: 918 Download: 451
Production of Plant Growth Promoting Antagonistic Rhizobacteria to Promote Cucumber Growth and Control Leaf Spot Disease (Corynespora cassiicola)
page: 72 - 82

Nuntavun Riddech[a]*, Kamon Sritongon[b]and Thanaporn Phibunwatthanawong[b]

Vol.44 No.1 (JANUARY 2017)
Research Article View: 921 Download: 375
Effects of Pediococcus pentosaceus PKWA-1 and Bacillus subtilis BA04 on Growth Performances, Immune Responses and Disease Resistance against Aeromonas hydrophila in Nile Tilapia (Oreochromis niloticus Linn.)
page: 997 - 1006

Saichai Kaew-on [a,b], Nontawith Areechon [c] and Penkhae Wanchaitanawong*[a,b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,372 Download: 276
Biofuel Production, Characterization and Degradation of 3-hydroxybutyate Methyl Ester from Polyhydroxybutyrate
page: 808 - 817

Kanokphorn Sangkharak*[a], Nisa Pichid [a], Tewan Yunu[a], Kamonthip Srinak [a], Sasithorn Sornnum [a] and Poonsuk Prasertsan [b]

Vol.43 No.4 (JULY 2016)
Research Article View: 936 Download: 374
Isolation and Screening of Lactobacillus plantarum Strains from Attieke Fermentation Process for A Starter Culture Development
page: 699 - 711

Krabi Ekoua Regina[a], Assamoi Allah Antoine*[a], Ehon Ayawovi Fafadzi[a], Brehima Diawara [b], Niamke Lamine Sebastien [a] and Thonart Philippe [c]

Vol.43 No.4 (JULY 2016)
Research Article View: 1,026 Download: 461
Production and Immobilization of Levansucrase
page: 44 - 51

Surawut Sangmanee, Santhana Nakapong, Kamontip Kuttiyawong and Rath Pichyangkura

Vol.42 No.1 (JANUARY 2015)
Research Article View: 926 Download: 305
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
page: 62 - 69

Saowanit Tongpim and Kenji Sakai

Vol.42 No.1 (JANUARY 2015)
Research Article View: 888 Download: 309
Screening of Volcanic Cave Actinomycetes for Antimicrobial Activity Against Paenibacillus larvae, A Honey Bee Pathogen
page: 26 - 33

Silvia Kay [a], Wasu Pathom-aree [b], and Naowarat Cheeptham*[a]

Vol.40 No.1 (JANUARY 2013)
Research Article View: 989 Download: 362
Nutritional and Physicochemical Qualities of Thua Nao (Thai Traditional Fermented Soybean)
page: 562 - 574

Katekan Dajanta1,2, Ekachai Chukeatirote3,* and Arunee Apichartsrangkoon2

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 936 Download: 342
Profiles of Plasmids in Lactobacilli Isolated from Fermented Foods
page: 648 - 652

Panitnart Auputinan, Yingmanee Tragoolpua, Sumalee Pruksakorn and Narumol Thongwai

Vol.38 No.4 (OCTOBER 2011)
Short Communication View: 1,047 Download: 271
Growth Inhibition of Ralstonia solanacearum PT1J by Antagonistic Bacteria Isolated from Soils in the Northern Part of Thailand
page: 345 - 354

Narumol Thongwai and Jirapa Kunopakarn

Vol.34 No.3 (SEPTEMBER 2007)
Research Article View: 1,009 Download: 452
Expression of Thermostable Lipase Gene from Bacillus stearothermophilus TP811 in E. coli M15 (pREP4)
page: 161 - 171

Padchanee Sangthong*[a], Supachok Sinchaikul [b], Hataichanoke Niamsup [a], Shui-Tein Chen [b] and Suree Phutrakul [a]

Vol.29 No.3 (DECEMBER 2002)
Research Article View: 1,403 Download: 268
Cloning and Expression of Protease Gene from Thermophilic Bacterium Bacillus stearothermophilus TLS33 in Escherichia coli DH5α
page: 53 - 59

Thammarat Kaosombat [a], Supachok Sinchaikul [b], Hataichanoke Niamsup [a], Shui – Tein Chen [b] and Suree Phutrakul* [a]

Vol.29 No.2 (AUGUST 2002)
Opinion View: 1,337 Download: 314
Modification of Culture Medium to Increase Production of Lipase from Bacillus stearothermophilus P1
page: 87 - 90

Bundit Boonsinthai [a], Wanna Thonabut [b], Jiraporn Nawarak [b], and Suree Phutrakul [b]

Vol.28 No.2 (DECEMBER 2001)
Opinion View: 1,478 Download: 262
β -Glucosidase Production by Common Split Gill Mushroom (Schizophyllum commune Fr.) in Submerged Culture
page: 82 - 88

Dararat Tongkao [a] and Thida Sripuan [b]

Vol.27 No.2 (DECEMBER 2000)
Opinion View: 3,806 Download: 445
Selection of Starter Cultures for the Production of Vegetarian Kapi, a Thai Fermented Condiment
page: 314 - 325

Suttida Wittanalai, Nopakarn Chandet, and Nuansri Rakariyatham

Vol.37 No.2 (MAY 2010)
Research Article View: 935 Download: 302
Outline
Figures