Growth Inhibition of Ralstonia solanacearum PT1J by Antagonistic Bacteria Isolated from Soils in the Northern Part of Thailand
Narumol Thongwai and Jirapa Kunopakarn* Author for corresponding; e-mail address: nthongw@hotmail.com
Volume: Vol.34 No.3 (SEPTEMBER 2007)
Research Article
DOI:
Received: 15 March 2007, Revised: -, Accepted: 26 June 2007, Published: -
Citation: Thongwai N. and Kunopakarn J., Growth Inhibition of Ralstonia solanacearum PT1J by Antagonistic Bacteria Isolated from Soils in the Northern Part of Thailand, Chiang Mai Journal of Science, 2007; 34(3): 345-354.
Abstract
The most serious infectious disease found in Siam Tulip or Patumma (alismatifolia isolates of the bacteria isolated from infected Patumma’s rhizomes, the isolate PT1J, which was identified as Patumma rhizomes upon re-infection. Concurrently, five of seventy-six bacterial isolates, screened for their ability to inhibit growth of minimizing growth of the pathogen; namely enterobacteria N9A and Ba3. Optimal conditions, for production of inhibitory substances from these antagonistic bacteria, were 30°C in a medium pH of 7, containing 0.25% (w/v) glucose and 2.0% (w/v) peptone. The promising time for introducing mixed cultures of these five antagonistic strains to Patummas grown in pots was at the beginning of Patumma cultivation, in order to prevent and protect the plants from CurcumaGagnep.) was a bacterial wilt caused by Ralstonia solanacearum. Among ninety-fourR. solanacearum, was selected for further study due to its high virulence againstR. solanacearum PT1J, showed high potential forBacillus sp. Ba4, N9C8 and N11IV, andR. solanacearum infection.