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  JOURNAL DETAIL



Miniaturized Green Analytical Method for Determination of Silver Ions Using C-phycocyanin from Cyanobacteria as an Ecofriendly Reagent


Paper Type 
Contributed Paper
Title 
Miniaturized Green Analytical Method for Determination of Silver Ions Using C-phycocyanin from Cyanobacteria as an Ecofriendly Reagent
Author 
Napaporn Youngvises*, Duy Hien Nguyen, Theppanya Charoenrat, Supaporn Kradtap-Hartwell, Jaroon Jakmunee and Awadh AlSuhaimi
Email 
nyoungvises@hotmail.com
Abstract:

 Green analytical chemistry methods have attracted increasing attention for sustainable

development in analytical laboratories. Miniaturization of analytical devices and replacement of
chemical regents with ecofriendly ones are key steps to realizing the objectives of green analytical
chemistry. This work describes a microfluidic-based micro-flow injection analysis (μ-FIA) system for
determination of silver ions (Ag+) in water samples using C-phycocyanin extracted from cyanobacteria
as a natural and safe colorimetric reagent. The μ-FIA system is comprised of a 30 × 30 square
polymethylmethacrylate platform on which the microchannel architecture has been engraved with a
CO2 laser. The microchip was sandwiched between two polydimethylsiloxane sheets for packaging
and to facilitate its integration with fiber optic spectrometer for signal monitoring, and a multi-syringe
pump for delivery/manipulation of solutions. At optimized operating conditions, the system response
was linear over the range 0.080–2.40 mg L−1 with a R2 of 0.9996, and the detection limit was 25 μg L−1.
The developed method was applied to analysis of Ag+ in drinking water, river water and wastes, and
the results were comparable with those from the standard inductively coupled plasma optical emission
spectrometry method. The outstanding features of this system are the low consumption of reagents
by the microfluidic device, and the replacement of toxic reagents with natural, ecofriendly alternatives.
Start & End Page 
221 - 230
Received Date 
2020-03-05
Revised Date 
Accepted Date 
2020-06-05
Full Text 
  Download
Keyword 
microfluidic, micro-flow injection analysis, natural reagent, silver ion, C-phycocyanin, green analytical chemistry
Volume 
Vol.48 No.1 (January 2021)
DOI 
Citation 
Youngvises N., Nguyen D.H., Charoenrat T., Kradtap-hartwell S., Jakmunee J. and Alsuhaimi A., Miniaturized Green Analytical Method for Determination of Silver Ions Using C-phycocyanin from Cyanobacteria as an Ecofriendly Reagent, Chiang Mai J. Sci., 2021; 48(1): 221-230.
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