Application of an Economic USB Digital Microscope for Microvolume Colorimetric Determination of Iron in Supplements with a Guava Leaf Extract

Miki Kanna, Suttida Luangton and Sarawut Somnam
* Author for corresponding; e-mail address: sarawut_som@cmru.ac.th
Volume: Vol.49 No.5 (September 2022)
Research Article
DOI: https://doi.org/10.12982/CMJS.2022.086
Received: 19 May 2022, Revised: 13 August 2022, Accepted: 17 August 2022, Published: -

Citation: Kanna M., Luangton S. and Somnam S., Application of an economic USB digital microscope for microvolume colorimetric determination of iron in supplements with a guava leaf extract. Chiang Mai Journal of Science, 2022; 49(5): 1365-1375. DOI 10.12982/CMJS.2022.086.

Abstract

The method for iron determination by using the microvolume of chemicals coupled with employing natural reagent was proposed towards green analytical chemistry. A simple, portable, and economic digital microscope was used to magnify the drop of 30 μL of product, that was mixed between sample and guava leaf extract reagent, to monitor the analytical signal as primitive color, red/ green/blue or RGB. The optimum conditions for the reagent extraction and the RGB detection were investigated. The calibration graph with linearity in the range of 40-120 mg L^-1 Fe(III) was obtained with the detection limit at 10.8 mg L^-1. High precision was achieved with %RSD less than 1.8%. The developed method was successfully applied to the assay of iron in supplement samples and found to be in good agreement with the results obtained by the FAAS method at the 95% confidence level.

Keywords: digital microscope, colorimetric determination, iron, natural reagent, RGB color system

Microwave-assisted Preparation of Magnetic S/Fe3O4 (SUFEO) Nanocomposite for Ag Nanoparticles Capture and Antimicrobial Applications
DOI: 10.12982/CMJS.2026.026.

Yong Hong Shiow, Khatijah Yusoff, Vuanghao Lim, Joon Ching Juan, Rafidah Zainon, Chui Fung Loke and Teck Hock Lim*

Vol.53 No.2 (March 2026)
Research Article View: 147 Download: 28

Environmental Interactions Shaping the Intestinal Microbiota of Cultured Sandfish (Holothuria scabra)
DOI: 10.12982/CMJS.2026.023.

Nittaya Jaeram, Aekkaraj Nualla-ong, Komwit Surachat and Warapond Wanna*

Vol.53 No.2 (March 2026)
Research Article View: 294 Download: 36

Synthesis and Characterization of Red Pigment from Acid Regeneration Plant (ARP) By-product Via Rod Milling Process
DOI: 10.12982/CMJS.2024.059.

Hai Song Woon, Keerthan Naidu, Lay Sheng Ewe, Lee Woen Ean and Kean Pah Lim

Vol.51 No.4 (July 2024)
Research Article View: 1,047 Download: 423

Polar Fungi Pseudogymnoascus: Secondary Metabolites and Ecological Significance
DOI: 10.12982/CMJS.2024.043.

Herland Satriawan, Teow Chong Teoh, Mohammed Rizman-Idid, Abiramy Krishnan, Nurlizah Abu Bakar and Siti Aisyah Alias

Vol.51 No.3 (May 2024)
Research Article View: 1,502 Download: 1,071

Effect of Molybdenum on the Corrosion Resistance of White Cast Iron Containing 27% Chromium
page: 1129 - 1140

Hoang Thi Ngoc Quyen, Nguyen Hong Hai and Nguyen Duong Nam

Vol.48 No.4 (July 2021)
Research Article View: 1,035 Download: 1,262

Miniaturized Green Analytical Method for Determination of Silver Ions Using C-phycocyanin from Cyanobacteria as an Ecofriendly Reagent
page: 221 - 230

Napaporn Youngvises*, Duy Hien Nguyen, Theppanya Charoenrat, Supaporn Kradtap-Hartwell, Jaroon Jakmunee and Awadh AlSuhaimi

Vol.48 No.1 (January 2021)
Research Article View: 1,613 Download: 789

Genetic Differentiation in Mrigal Carp (Cirrhinus cirrhosus) Affected by Heavy Metals Near Iron Ore Mine
page: 100 - 111

Sutee Chowrong, Bundit Tengjaroenkul and Lamyai Neeratanaphan*

Vol.48 No.1 (January 2021)
Research Article View: 1,015 Download: 560

Iron(II) Complexes Containing 1,2,4-Triazole-Pyridine and the Substituted Derivatives; Electronic Properties and Magnetic Moments
page: 1068 - 1080

Kristian Handoyo Sugiyarto, Cornelia Budimarwanti and Harold Andrew Goodwin

Vol.47 No.5 (September 2020)
Research Article View: 1,139 Download: 733

Application of Smartphone as a Digital Image Colorimetric Detector for Batch and Flow-based Acid-Base Titration
page: 975 - 986

Janejira Chanla, Miki Kanna, Jaroon Jakmunee, and Sarawut Somnam*

Vol.46 No.5 (September 2019)
Research Article View: 1,124 Download: 610

Electronic Properties of Iron(II) Complexes of 2-(Pyridin-2-yl)benzoxazole
page: 756 - 765

Kristian Handoyo Sugiyarto* and Harold Andrew Goodwin

Vol.46 No.4 (July 2019)
Research Article View: 932 Download: 507

Electrocoagulation of Some Heavy Metals
page: 33 - 40

Kanlaya Jumpatong and Duang Buddhasukh*

Vol.30 No.1 (APRIL 2003)
Research Article View: 1,596 Download: 405

Solid Phase-assisted Determination of trace Copper(ll) and Iron(lll) in Food and Water Samples by Cloud Point Extraction: Response Surface Methodology Optimization
page: 1960 - 1974

Farzin Nekouei and Shahram Nekouei

Vol.45 No.4 (July 2018)
Research Article View: 1,121 Download: 307

Iron Reduces Iron: A Spectroelectrochemical Insight of Ligand Effect on Iron Redox Potential
page: 1087 - 1098

Shabnum Bashir, Mohammad Mustafa, Syed Wasim Safvi, Nisar Ahmad Farhad and Masood Ahmad Rizvi*

Vol.45 No.2 (March 2018)
Research Article View: 984 Download: 535

Effect of Calcination Temperature on the Magnetic Characteristics of Synthetic Iron Oxide Magnetic Nanoparticles for Arsenic Adsorption
page: 528 - 539

Nucharee Chomchoey, Darunee Bhongsuwan and Tripob Bhongsuwan

Vol.45 No.1 (January 2018)
Research Article View: 1,421 Download: 373

A Simple High-performance Liquid Chromatography Coupled to Fluorescence Detection Method using Column-switching Technique for Measuring Urinary 1-hydroxypyrene from Environmental Exposure
page: 1441 - 1452

Kunrunya Sutan*, Warangakana Naksen* and Tippawan Prapamontol+

Vol.44 No.4 (October 2017)
Research Article View: 1,092 Download: 405

Detection of Helicobacter pylori in Aquatic Environments and Drinking Waters in Northeastern Thailand
page: 731 - 741

Chariya Chomvarin*, Warawan Wongboot, Aschana Tirapattanun, Sakawrat Kanthawong, Suwin Wongwajana,

Vol.44 No.3 (July 2017)
Research Article View: 947 Download: 528

Protein Biomarker Screening on Effect of Freeze Dried Crocodile Blood and Vitamin C in Iron-deficient Anemic Rats
page: 1077 - 1088

Ekawit Threenet [a], Win Chaeychomsri [b], Sudawan Chaeychomsri [c] and Jindawan Siruntaweneti* [b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,455 Download: 429

Application of SYBR Green Real-Time PCR for Detection of Toxigenic Vibrio cholerae O1 in the Aquatic Environment
page: 588 - 598

Aschana Tirapattanun, Chariya Chomvarin, Warawan wongboot and Boonnapa Kanoktippornc

Vol.42 No.3 (JULY 2015)
Research Article View: 1,000 Download: 316

An Agent Model for Information Filtering using Revolutionary RSVD Technique
page: 1429 - 1438

Dussadee Praserttitipong [a] * and Peraphon Sophatsathit [b]

Vol.41 No.5/2 (OCTOBER 2014)
Research Article View: 921 Download: 272

Applying of Aluminium Deoxidation in Iron Roll Casting
page: 196 - 198

Teerapong Harnwirojkul, Mana Polboon, John Pearce and Thanaporn Korad

Vol.35 No.1 (JANUARY 2008)
Research Article View: 898 Download: 337

Seismic Stratigraphy of the Fang Basin, Chiang Mai, Thailand
page: 77 - 82

Rungsun Nuntajun

Vol.36 No.1 (JANUARY 2009)
Research Article View: 1,068 Download: 369

Developing Indicators for Floodplain Wetlands: Managing Water in Agricultural Landscapes
page: 224 - 235

Kim Jenkins, Richard Kingsford, and Darren Ryder

Vol.36 No.2 (MAY 2009)
Short Communication View: 953 Download: 375

Hepatotoxin Biosynthesis and Regulation in Cyanobacteria – the Putative Involvement of Nitrogen and Iron Homeostasis Mechanisms
page: 200 - 223

Hannah P. Ginn, Leanne A. Pearson and Brett A. Neilan

Vol.36 No.2 (MAY 2009)
Short Communication View: 1,048 Download: 254

Characterization of Perovskite Lead Iron Niobate Powders Synthesized by Partial Oxalate
page: 71 - 76

Pitak Youmee [a] and Sukon Phanichphant* [b]

Vol.32 No.1 (JANUARY 2005)
Short Communication View: 1,388 Download: 249

Chiang Mai Journal of Science

1,647

Q3 0.80

Q3 1.3

7 days

Outline

Figures and Tables