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Chiang Mai Journal of Science, Faculty of Science, Chiang Mai University
 


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Application of an Economic USB Digital Microscope for Microvolume Colorimetric Determination of Iron in Supplements with a Guava Leaf Extract


Paper Type 
Contributed Paper
Title 
Application of an Economic USB Digital Microscope for Microvolume Colorimetric Determination of Iron in Supplements with a Guava Leaf Extract
Author 
Miki Kanna, Suttida Luangton and Sarawut Somnam
Email 
sarawut_som@cmru.ac.th
Abstract:

     The method for iron determination by using the microvolume of chemicals coupled with employing natural reagent was proposed towards green analytical chemistry. A simple, portable, and economic digital microscope was used to magnify the drop of 30 μL of product, that was mixed between sample and guava leaf extract reagent, to monitor the analytical signal as primitive color, red/ green/blue or RGB. The optimum conditions for the reagent extraction and the RGB detection were investigated. The calibration graph with linearity in the range of 40-120 mg L-1 Fe(III) was obtained with the detection limit at 10.8 mg L-1. High precision was achieved with %RSD less than 1.8%. The developed method was successfully applied to the assay of iron in supplement samples and found to be in good agreement with the results obtained by the FAAS method at the 95% confidence level.

Start & End Page 
1365 - 1375
Received Date 
2022-05-19
Revised Date 
2022-08-13
Accepted Date 
2022-08-17
Full Text 
  Download
Keyword 
digital microscope, colorimetric determination, iron, natural reagent, RGB color system
Volume 
Vol.49 No.5 (September 2022)
DOI 
https://doi.org/10.12982/CMJS.2022.086
Citation 
Kanna M., Luangton S. and Somnam S., Application of an Economic USB Digital Microscope for Microvolume Colorimetric Determination of Iron in Supplements with a Guava Leaf Extract, Chiang Mai Journal of Science, 2022; 49(5): 1365-1375. DOI 10.12982/CMJS.2022.086.
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