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Zearalenone Contamination in Corn for Human Consumption in Kamphaengphet, Thailand
Paper Type  |
Contributed Paper |
Title |
Zearalenone Contamination in Corn for Human Consumption in Kamphaengphet, Thailand |
Author |
Wachira Singkong*[a], Hastin Ratanapol [a] and Wachira Liamkaew [b] |
Email |
champsing@hotmail.com |
|
Abstract: This study was a survey of estrogenic mycotoxin zearalenone (ZEA), which dangerously causes endometrial hyperplasia and cancer of esophagus in human body, method with an immuno-affinity column for clean-up. Before the measurement of the confirmed sample, the HPLC method was validated using a blank corn kernel matrix. The average recovery of blank corn kernel spiked with ZEA at levels of 100-550 mg/kg was 98.2%. The detection was confirmed by fluorescence spectral scanning throughout the chromatographic running. The method’s limitation of the detection was 3 mg/kg for ZEA in corn samples and the linear range applicable for analyses was 5-700 mg/kg of ZEA. Of 17 samples confirmed by HPLC, 14 samples were observed to be contaminated in a comparable range from 8.0 to 301 mg/kg. The comparative analysis of 17 positive of corn kernel samples used in ELISA and HPLC methods showed a sensitive correlation (R2=0.9538). |
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Start & End Page |
534 - 539 |
Received Date |
2012-06-05 |
Revised Date |
|
Accepted Date |
2012-12-26 |
Full Text |
Download |
Keyword |
zearalenone, corn, ELISA and HPLC |
Volume |
Vol.40 No.3 (JULY 2013) |
DOI |
|
Citation |
Singkong*[a] W.., [a] H.R. and [b] W.L., Zearalenone Contamination in Corn for Human Consumption in Kamphaengphet, Thailand, Chiang Mai Journal of Science, 2013; 40(3): 534-539. |
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