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Impact of Rhizobacterial Inoculants and Organic Amendment on Bacterial Communities of Jerusalem artichoke Tissue and Rhizosphere by using Denaturing Gradient Gel Electrophoresis


Paper Type 
Contributed Paper
Title 
Impact of Rhizobacterial Inoculants and Organic Amendment on Bacterial Communities of Jerusalem artichoke Tissue and Rhizosphere by using Denaturing Gradient Gel Electrophoresis
Author 
Natthawat Sritongon, Sophon Boonlue, Wiyada Mongkolthanaruk, Sanun Jogloy and Nuntavun Riddech
Email 
nunrid@kku.ac.th
Abstract:

      The application of organic amendments can improve soil quality and influence bacterial communities, contributing to enhanced soil health in agricultural systems. This study investigated the effect of using rain tree leaf, corn husk, and peat moss as soil amendments, supplemented with plant growth promoting rhizobacteria on communities of bacteria in the tissue and rhizosphere of Jerusalem artichoke. The plants were observed for 120 days after transplanting. Polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) technique was used to investigate the impact of both organic amendments and bacterial consortium with comparative profiles. Comparison DGGE profile by clustering analysis did not clearly separate each organic amendment and bacterial inoculation, but principal component analysis (PCA) revealed that plant age had an influence on tissue and rhizosphere bacterial communities. The results indicated that soil amendments and bacterial inoculation had no effect on bacterial community shifts. Interestingly, the genera of bacteria able to most colonize in the tissue and rhizosphere were the uncultured bacteria. Thus, we provide promising data for boosting Jerusalem artichoke growth and soil quality with potential organic amendments to improve soil with sustainable strategies.

Article ID
e2023051
Received Date 
2023-05-22
Revised Date 
2023-07-24
Accepted Date 
2023-08-21
Full Text 
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Keyword 
soil amendments, rain tree leaves, corn husks, peat moss, plant growth promoting rhizobacteria
Volume 
Vol.50 No.5 (September 2023)
DOI 
https://doi.org/10.12982/CMJS.2023.051
Citation 
Sritongon N., Boonlue S., Mongkolthanaruk W., Jogloy S. and Riddech N., Impact of Rhizobacterial Inoculants and Organic Amendment on Bacterial Communities of Jerusalem artichoke Tissue and Rhizosphere by using Denaturing Gradient Gel Electrophoresis, Chiang Mai J. Sci., 2023; 50(5): e2023051. DOI 10.12982/CMJS.2023.051.
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