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Preparation and Performance Test of a Simplified Screen-Printed Carbon Electrode for Use as a Chronoamperometric Transducer in Genosensor


Paper Type 
 Contributed Paper
Title 
 Preparation and Performance Test of a Simplified Screen-Printed Carbon Electrode for Use as a Chronoamperometric Transducer in Genosensor
Author 
 Low Kim Fatt [a], Kritsanaporn Chuenrangsikul [b], Patsamon Rijiravanich [b], Werasak Surareungchai
Email 
 yeancyn@yahoo.com
Abstract:

In this work, a simplified two-electrode configuration metal-free screen-printed carbon electrode (2-MFSPCE) was fabricated in-house and electrochemically evaluated. The electrode performance of the 2-MFSPCE was preliminary characterized with ferricyanide by using cyclic voltammetry (CV). The electroactivity of  TMB (3,3’,5,5’-tetramethylbenzidine) substrate on 2-MFSPCE was studied before the development of a Horseradish peroxidase (HRP) enzyme-based electrochemical genosensor detection platform for specific 40 mer-synthetic target DNA sequence from food-borne pathogen, Vibrio cholerae lolB gene. The CV results showed that the electron transfer/conductivity on 2-MFSPCE was highly consistent and stable without any significant shifting. In addition, there was good electrochemical reproducibility that all individual 2-MFSPCE (within and between batches of fabrication) showed relative low variation in electroanalysis. A detection potential (reduction potential) of ­­−0.1 V was empirically determined on 2-MFSPCE for enzymatic product from HRP/TMB catalytic reaction. By using two-step sandwich-type hybridization strategy, the chronoamperometric analysis of the enzyme-based electrochemical genosensor with 2-MFSPCE as transducer bestowed a detection limit of 5 fM of target sequence. The encouraging results obtained from the electrochemical characterization and the well response in electrochemical genosensor study indicating the feasibility of this 2-MFSPCE to use as a low-cost disposable electrode in electrochemical sensor application. 

Start & End Page 
 610 - 622
Received Date 
 2011-08-22
Revised Date 
Accepted Date 
 2012-06-24
Full Text 
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Keyword 
 two-electrode configuration, metal-free screen-printed carbon electrode, enzyme-based electrochemical genosensor, sandwich-typed hybridization
Volume 
 Vol.39 No.4 (OCTOBER 2012)
DOI 
Citation 
 [a] L.K.F., [b] K.C., [b] P.R. and Surareungchai W., Preparation and Performance Test of a Simplified Screen-Printed Carbon Electrode for Use as a Chronoamperometric Transducer in Genosensor , Chiang Mai Journal of Science, 2012; 39(4): 610-622.
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