Journal Volumes


Visitors
ALL : 2,095,349
TODAY : 3,693
ONLINE : 234



















  JOURNAL DETAIL



Analytical Validation of Sildenafil Citrate Inhaler Preparation


Paper Type 
Contributed Paper
Title 
Analytical Validation of Sildenafil Citrate Inhaler Preparation
Author 
Somchai Sawatdee [a] and Teerapol Srichana* [a,b]
Email 
teerapol.s@psu.ac.th
Abstract:

The aim of this study was to develop and validate a high performance liquid chromatographic (HPLC) system for the analysis of sildenafil citrate in pharmaceutical inhaler preparations. Sildenafil was partially degraded by acid and alkali and was completely degraded by oxidation. Direct exposure to heat and UV in solid state had no effect on the degradation of sildenafil. This method was able to resolve any degradation products from the sildenafil peak. Sildenafil citrate showed UV absorption maximum at 240 and 276 nm. A reversed phase HPLC system for the separation and quantitation of sildenafil citrate assay was successfully developed and validated. The chromatographic conditions employed a C18 column (4.6 x 250 mm) with a mobile phase of 0.2 M ammonium acetate buffer and acetonitrile (40:60 v/v, pH 7.0) at a flow rate of 1.0 mL/min with UV detection at 240 nm. The retention time of sildenafil citrate was about 5 min. The method was specific to the sildenafil at the presence of other common excipients in the inhaler preparation. The method was accurate (99.5% recovery) and precise for both intra-day (0.5-1.0%) and inter-day (1.1%) assays. A linear correlation was obtained over the concentration range of 0.5-500 mg/mL (r2=1.0). The sample solution from metered dose inhalers (MDIs) was stable over 24 h. The LOD and LOQ were 1.30 and 6.10 ng/mL, respectively.

Start & End Page 
669 - 680
Received Date 
2012-12-13
Revised Date 
Accepted Date 
2013-02-15
Full Text 
  Download
Keyword 
sildenafil citrate, validation, liquid chromatography, degradation
Volume 
Vol.40 No.4 (OCTOBER 2013)
DOI 
Citation 
Sawatdee S. and Srichana T., Analytical Validation of Sildenafil Citrate Inhaler Preparation, Chiang Mai J. Sci., 2013; 40(4): 669-680.
SDGs
View:535 Download:155

  RELATED ARTICLE

Degradation of Dimethyl Phthalate and Diethyl Phthalate by Bacteria Isolated from Jazan, Saudi Arabia
Article ID: e2024025
Author:Nouf A. AL Awaji, Mohammed D. Y. Oteef and Ashraf M. M. Essa
Vol.51 No.2 (March 2024) View: 448 Download:276
Screening of Cellulose and Pectin Degrading Actinomycetes and Evaluation of the Ability of Composite Flora to Degrade Corn Straw
Article ID: e2023021
Author:Xiaofei Liu, Jie Lian, Zhihui Zheng, Yuena Qi, Guang Zhang, Wei Wang and Na Zhang
Vol.50 No.3 (May 2023) View: 654 Download:330
N, N-Dimethylformamide-assisted One-step Synthesis of Fe Doped Hierarchical Flower-like BiOCOOH with Enhenced Photocatalytic Activity
Article ID: e2023007
Author:Piao Chen, Mingqing Shan, Xiongjian Li, Shuijin Yang and Yun Yang
Vol.50 No.1 (January 2023) View: 399 Download:258
Potential of Vetiver (Chrysopogon zizanioides L.), Inoculated With Arbuscular Mycorrhizal Fungi, To Improve Soil Quality in Degraded Soil
page: 1247 - 1258
Author:Dinuka Lakmali, Samantha C. Karunarathna, Lucas A.P. Dauner and Neelamanie Yapa
Vol.48 No.5 (September 2021) View: 840 Download:313
Simultaneous Determination of Nonsteroidal Anti-Inflammatory Drugs Using Vortex-Assisted Liquid-Liquid Microextraction With Back Extraction Coupled With HPLC-UV
page: 1363 - 1373
Author:Rania Edrees Adam Mohammad, Elbaleeq Adam Gubartallah, Abdalla Ahmed Elbashir, Bahruddin Saad, Noorfatimah Yahaya, Nur Nadhirah Mohamad Zain, Nurul Yani Rahim and Mazidatulakmam Miskam
Vol.48 No.5 (September 2021) View: 790 Download:250
Development and Validation of a Simple High Performance Liquid Chromatography/UV Method for Simultaneous Determination of Urinary Phthalates in Human Urine
page: 1374 - 1380
Author:Thawiphark Thadsri, Kotchaphan Chooluck, Jiraporn Chingunpitak, Vichit Supornsilchai and Wichit Nosoongnoen
Vol.48 No.5 (September 2021) View: 656 Download:237
Salen-Porphyrin Complex-Catalyzed Degradation of Chlorophene by Hydrogen Peroxide
page: 1079 - 1088
Author:Xujie Lu, Liang Wang, Xiao-Jun Yang, Rui-Na Liu and Xue-Fei Zhou
Vol.48 No.4 (July 2021) View: 604 Download:162
Effects of Sub-lethal Concentrations of Isothiazolone Biocide on the Performance of Rotating Biological Contactors
page: 74 - 89
Author:Prapaparn Sirikhansaeng, Niphaphat Phukoetphim, Pattana Laopaiboon and Lakkana Laopaiboon*
Vol.48 No.1 (January 2021) View: 604 Download:401
Poly(L-lactide)-Degrading Enzyme from Laceyella sacchari LP175: Cloning, Sequencing, Expression, Characterization and Its Hydrolysis of Poly(L-lactide) Polymer
page: 417 - 430
Author:Thanasak Lomthong, Marie Guicherd, Gianluca Cioci, Sophie Duquesne, Alain Marty, Saisamorn Lumyong and Vichien Kitpreechavanich
Vol.46 No.3 (May 2019) View: 624 Download:319
High Performance Liquid Chromatography Incorporating to Short Column and Micellar Mobile Phase for Determination of Some Contraceptive Drugs
page: 118 - 131
Author:Thanatcha Chaida, Suchada Udomwatwong, Warawut Tiyapongpattana and Napaporn Youngvises
Vol.46 No.1 (January 2019) View: 544 Download:281
Preparation and Degradation Behavior of Polycaprolactone Nanoporous Membrane
page: 2790 - 2796
Author:Naini Guo
Vol.45 NO.7 (November 2018) View: 627 Download:417
Hollow-fiber Microextraction Combined with Hydrophilic Interaction Liquid Chromatography Mass Spectrometry for Analytical Determination of High Polarity Herbicides in Water
page: 2381 - 2396
Author:Siripastr Jayanta and Natchanun Leepipatpiboon
Vol.45 No.6 (September 2018) View: 651 Download:404
Self-setting Calcium Phosphate Enhanced with Osteoconduction and Bioactivity for Bone Cement
page: 2132 - 2139
Author:Sirirat Tubsungnoen Rattanachan, Sanong Suksaweang, Ting-Xin Jiang, Randall B. Widelitz, Cheng-Ming Chuong and Nuan La-ong Srakaew
Vol.45 No.5 (Special 2018) View: 639 Download:384
Synthesis and Characterization of Mechanochemically Synthesized Cobalt Oxide and Evaluation of its Catalytic Performance for Degradation of Congo Red Dye in Aqueous Medium
page: 1901 - 1908
Author:Mohsin Siddique, Mohammad Ilyas and Muhammad Saeed
Vol.45 No.4 (July 2018) View: 644 Download:185
Estimation of Sodium Lauryl Sulphate Concentration in Marketed Formulations by Stability Indicating ‘Green’ Planar Chromatographic Method
page: 1531 - 1542
Author:Nazrul Haq*, Nasir A. Siddiqui, Perwez Alam, Faiyaz Shakeel, Fars K. Alanazi and Ibrahim A. Alsarra
Vol.45 No.3 (May 2018) View: 614 Download:258
Ascorbic Acid Application Improves Salinity Stress Tolerance in Wheat
page: 1296 - 1306
Author:Manzer H Siddiqui*, Saud A Alamri, Mutahhar YY Al-Khaishany, Mohammed A Al-Qutami and Hayssam M Ali
Vol.45 No.3 (May 2018) View: 565 Download:249
Strategic Applications and the Challenges of Subcritical Water Extraction Technology in Food Industries
page: 1015 - 1029
Author:Erasto Mlyuka, Martha Mbifile, Shuang Zhang, Zongping Zheng and Jie Chen*
Vol.45 No.2 (March 2018) View: 579 Download:409
Development and Validation of Atomic Absorption Spectroscopic Method for Analysis of ZnO in Blemish Balm Cream
page: 404 - 412
Author:Sophi Damayanti, Tuan Norhafiza Binti Tuan Mat, Pimpichaya Tiyasuwan, Ondrej Bolcarovic, Wipawadee Yoo-in, Andhika B. Mahardhika and Supat Jiranusornkul
Vol.45 No.1 (January 2018) View: 649 Download:362
Comparison and Characterization of Purified Cellulase and Xylanase from Bacillus amyloliquefaciens CX1 and Bacillus subtilis B4
page: 92 - 105
Author:Kalawong Ratchara, Wakayama Mamoru, Anuntalabhochai Somboon, Wongsawad Chalobol and Sangwijit Kant
Vol.45 No.1 (January 2018) View: 576 Download:169
ZnO Catalyzed Degradation of Methyl Orange in Aqueous Medium
page: 1646 - 1653
Author:Muhammad Saeed*, Shahid Adeel, Hafiz Abdur-raoof, Muhammad Usman, Asim Mansha, Aziz Ahmad and Muhamm
Vol.44 No.4 (October 2017) View: 646 Download:245
Qualitative and Quantitative Analysis of Tildipirosin by Ultra-performance Hydrophilic Interaction Liquid Chromatography-tandem High Resolution Quadrupole Time-of-flight Mass Spectrometry
page: 956 - 964
Author:Jiaxin Wu*, Min Liu, Fei Shang, Chuanliang Zhang, Min Song, Peng Qi and Jingle Liang
Vol.44 No.3 (July 2017) View: 634 Download:205
Removal of Microcystin from Lake Water Using an Internal Airlift Loop Ceramic Honeycomb Support Bioreactor
page: 1048 - 1058
Author:Theerasak Somdee* [a], Anchana Somdee [a], John Ruck [b], Margaret Allison [b] and Rachel Page [b]
Vol.43 No.5 (OCTOBER 2016) View: 528 Download:607
Effect of Temperature on Schizophyllum commune Growth and 4H-pyran-4-one,2,3-dihydro-3, 5-dihydroxy-6-methyl- Production using a Bubble Column Bioreactor
page: 462 - 469
Author:Yi Peng Teoh and Mashitah Mat Don*
Vol.43 No.3 (APRIL 2016) View: 535 Download:237
Model-free Kinetic Analysis of Thermal Degradation of Polystyrene and High-Density Polyethylene Blends
page: 296 - 305
Author:Kasawat Tippaha, Nichaphat Jittangkoon and Parimanan Cherntongchai*
Vol.43 No.2 (SPECIAL ISSUE 1) View: 608 Download:177
Effect of temperature on Schizophyllum commune growth and 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- production using a bubble column bioreactor
page: 539 - 548
Author:Yi Peng, Teoh and Mashitah, Mat Don
Vol.42 No.3 (JULY 2015) View: 548 Download:217
Micellar Liquid Chromatographic Determination of Sildenafil Citrate in Pharmaceutical Formulations
page: 408 - 418
Author:Sujaritpun Jaipang [a], Dammrong Santiarworn [a], Saisunee Liawruangrath [b] and Boonsom Liawruangra
Vol.40 No.3 (JULY 2013) View: 519 Download:196
Poly(lactide) Degradation By Pseudocardia alni AS4.1531
page: 128 - 132
Author:Maytiya Konkit, Amnat Jarerat, Chartchai Kamongnuch, Saisamorn Lumyong and Wasu Pathom-aree
Vol.39 No.1 (JANUARY 2012) View: 614 Download:159
Application of Horseradish Peroxidase Mediated Oxidative Coupling Reaction for the Determination of Mesalazine
page: 551 - 559
Author:Shaik Shakeela, Bandakavi Sita Ram, Chandra Bala Sekaran
Vol.38 No.4 (OCTOBER 2011) View: 529 Download:201
Extraction and Determination of Organochlorine and Carbamate Pesticides in Sediment Samples
page: 83 - 85
Author:Wanna Kanchanamayoon, Sirichai Hathongkam and Apirak Pongchamnong.
Vol.28 No.2 (DECEMBER 2001) View: 583 Download:182
Solid Phase Extraction of Carbamate and Organochlorine Pesticides in Water
page: 9 - 15
Author:Wanna Kanchanamayoon, Sirichai Hathongkam and Amnat Laothong
Vol.28 No.1 (JUNE 2001) View: 554 Download:187



Search in this journal


Document Search


Author Search

A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z

Popular Search






Chiang Mai Journal of Science

Faculty of Science, Chiang Mai University
239 Huaykaew Road, Tumbol Suthep, Amphur Muang, Chiang Mai 50200 THAILAND
Tel: +6653-943-467




Faculty of Science,
Chiang Mai University




EMAIL
cmjs@cmu.ac.th




Copyrights © Since 2021 All Rights Reserved by Chiang Mai Journal of Science