Application of Horseradish Peroxidase Mediated Oxidative Coupling Reaction for the Determination of Mesalazine
Shaik Shakeela, Bandakavi Sita Ram, Chandra Bala Sekaran* Author for corresponding; e-mail address: balumphil@gmail.com
Volume: Vol.38 No.4 (OCTOBER 2011)
Research Article
DOI:
Received: -, Revised: -, Accepted: -, Published: -
Citation: Shakeela S., Ram B.S. and Sekaran C.B., Application of Horseradish Peroxidase Mediated Oxidative Coupling Reaction for the Determination of Mesalazine, Chiang Mai Journal of Science, 2011; 38(4): 551-559.
Abstract
This work reports three novel spectrophotometric methods for the determination of mesalazine in bulk and pharmaceutical dosage forms. The proposed methods are based on the oxidative coupling of mesalazine with orcinol (method A), resorcinol (method B) and cresol (method C) in the presence of hydrogen peroxide and horseradish peroxidase to produce a colored complex having absorption maxima at 490 nm, 470 nm and 480 nm, respectively. The Beer’s law is obeyed in the concentration range of 5-30 mg/ml of the drug for all the three methods. The methods were validated statistically and by recovery studies. The proposed methods are applied to marketed tablet formulations. The results obtained from tablet formulations compared well with those obtained by the official method and demonstrated good accuracy and precision.