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Application of Horseradish Peroxidase Mediated Oxidative Coupling Reaction for the Determination of Mesalazine


Paper Type 
 Contributed Paper
Title 
 Application of Horseradish Peroxidase Mediated Oxidative Coupling Reaction for the Determination of Mesalazine
Author 
 Shaik Shakeela, Bandakavi Sita Ram, Chandra Bala Sekaran
Email 
 balumphil@gmail.com
Abstract:

             This  work  reports  three  novel  spectrophotometric  methods  for  the  determination  of  mesalazine  in  bulk  and  pharmaceutical  dosage  forms. The  proposed  methods  are  based  on  the  oxidative  coupling  of  mesalazine  with  orcinol  (method A),  resorcinol  (method B)  and  cresol  (method C)  in  the  presence  of  hydrogen  peroxide  and  horseradish  peroxidase  to  produce  a  colored  complex  having  absorption  maxima  at  490  nm, 470  nm  and  480  nm,  respectively. The  Beer’s  law  is  obeyed  in  the  concentration  range of  5-30  mg/ml  of  the  drug  for  all the  three  methods. The  methods  were  validated  statistically  and  by  recovery  studies. The  proposed  methods  are  applied to  marketed  tablet  formulations. The  results  obtained  from  tablet  formulations  compared  well  with  those  obtained  by  the  official  method  and  demonstrated  good  accuracy  and  precision. 

 
Start & End Page 
 551 - 559
Received Date 
Revised Date 
Accepted Date 
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Keyword 
 horseradish peroxidase, mesalazine, spectrophotometry, Beer' law, validation
Volume 
 Vol.38 No.4 (OCTOBER 2011)
DOI 
Citation 
 Shakeela S., Ram B.S. and Sekaran C.B., Application of Horseradish Peroxidase Mediated Oxidative Coupling Reaction for the Determination of Mesalazine, Chiang Mai Journal of Science, 2011; 38(4): 551-559.
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