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High Performance Liquid Chromatographic Analysis of Asiaticoside in Centella asiatica (L.) Urban


Paper Type 
Short Communication
Title 
High Performance Liquid Chromatographic Analysis of Asiaticoside in Centella asiatica (L.) Urban
Author 
Prateek K. Jain and Ram K. Agrawal
Email 
dragrawal2001@yahoo.co.in
Abstract:
A simple, sensitive high performance liquid chromatographic method was developed for the determination of  asiaticoside in Centella asiatica extract. The asiaticoside yielded 1.7 mg/100 mg of the test extract. Calibration graph of asiaticoside showed good linearity in the concentration range of (32 - 1027 μg/mL), with a correlation coefficient of 0.999. The average recovery for asiaticoside is 97.5 %. The proposed method is reproducible and statistically validated. The system was successfully used to investigate the presence of the asiaticoside in Centella asiatica plant parts.
Start & End Page 
521 - 525
Received Date 
2008-03-03
Revised Date 
Accepted Date 
2008-04-18
Full Text 
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Keyword 
Centella asiatica, asiaticoside, HPLC method
Volume 
Vol.35 No.3 (SEPTEMBER 2008)
DOI 
Citation 
Jain P.K. and Agrawal R.K., High Performance Liquid Chromatographic Analysis of Asiaticoside in Centella asiatica (L.) Urban, Chiang Mai J. Sci., 2008; 35(3): 521-525.
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Chiang Mai Journal of Science

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