High Performance Liquid Chromatographic Analysis of Asiaticoside in Centella asiatica (L.) Urban
Prateek K. Jain and Ram K. Agrawal* Author for corresponding; e-mail address: dragrawal2001@yahoo.co.in
Volume :Vol.35 No.3 (SEPTEMBER 2008)
Short Communication
DOI:
Received: 3 March 2008, Revised: -, Accepted: 18 April 2008, Published: -
Citation: Jain P.K. and Agrawal R.K., High Performance Liquid Chromatographic Analysis of Asiaticoside in Centella asiatica (L.) Urban, Chiang Mai Journal of Science, 2008; 35(3): 521-525.
Abstract
A simple, sensitive high performance liquid chromatographic method was developed for the determination of asiaticoside in Centella asiatica extract. The asiaticoside yielded 1.7 mg/100 mg of the test extract. Calibration graph of asiaticoside showed good linearity in the concentration range of (32 - 1027 μg/mL), with a correlation coefficient of 0.999. The average recovery for asiaticoside is 97.5 %. The proposed method is reproducible and statistically validated. The system was successfully used to investigate the presence of the asiaticoside in Centella asiatica plant parts.