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Antifungal Activity of the Essential Oil Extracted from Zanthoxylum piperitum Seeds Against Aspergillus flavus


Paper Type 
Contributed Paper
Title 
Antifungal Activity of the Essential Oil Extracted from Zanthoxylum piperitum Seeds Against Aspergillus flavus
Author 
Chorpaka Phuangsri [a,b], Nuchnipa Nuntawong [b], Hataichanoke Niamsup* [b]
Email 
hataichanoke.n@cmu.ac.th
Abstract:
The antifungal activity of essential oil extracted from Zanthoxylum piperitum seeds was tested and the oil's potential as a food preservative was evaluated. The main chemical components determined in the oil were β-phelladrene (23.2%), sabinene (14.6%), and brevifolin (11.9%). The oil's antifungal activity was tested in solid and liquid cultures of aflatoxigenic Aspergillus flavus. The minimum inhibitory concentration of oil was 4.5 µL/mL. The possible actions against A. flavus are distortion in conidial heads and decrease of hyphal diameters as observed under a light microscope. A reduction in the ergosterol content in fungal plasma membrane by 34.41 % also occurred. The use of this essential oil as an antifungal coat was tested on dried bird chili. The essential oil at 4.5 and 9.0 µL/mL reduced fungal development on chili peel to 45.20 % and 89.32 %, respectively. Thus, application as a food preservative is possible.

Start & End Page 
584 - 594
Received Date 
2015-02-16
Revised Date 
Accepted Date 
2015-05-21
Full Text 
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Keyword 
Aspergillus flavus, Ergosterol, Essential oil, Antifungal activity, Zanthoxylum piperitum
Volume 
Vol.44 No.2 (April 2017)
DOI 
Citation 
Phuangsri C., Nuntawong N. and Niamsup H., Antifungal Activity of the Essential Oil Extracted from Zanthoxylum piperitum Seeds Against Aspergillus flavus , Chiang Mai J. Sci., 2017; 44(2): 584-594.
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Chiang Mai Journal of Science

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