In vitro Study on antifungal activity of Achillea wilhelmsii flower essential oil against twenty strains of Candida albicans
Leila Amjad*[a], Kobra Mousavidehmourdi [b], Zahra Rezvani [c]* Author for corresponding; e-mail address: Amjad.leila@gmail.com; Amjad@iaufala.ac.ir.
Volume: Vol.41 No.5/1 (OCTOBER 2014)
Research Article
DOI:
Received: 22 April 2013, Revised: -, Accepted: 30 May 2013, Published: -
Citation: Amjad L., Mousavidehmourdi K. and Rezvani Z., In vitro Study on antifungal activity of Achillea wilhelmsii flower essential oil against twenty strains of Candida albicans , Chiang Mai Journal of Science, 2014; 41(5/1): 1058-1064.
Abstract
Infection diseases always were important worry. In the past decade numerous reports of treatment failures are reported. It is important to increase effective therapy. The aim of this study was in vitro studing of antifungal effects of the flowers essential oil of Achillea wilhelmsii C. Koch. In this study essential oil was obtained by hydro-distillation using Clevenger type apparatus for 3h. It was tested in vitro against 20 fungi strains. Several dilution of essential oil (10%, 15%, and 20%) was prepared. Antifungal activity was tested by using microdilution MIC determination, disk diffusion assay and agar well diffusion assay. Data were analyzed by ANOVA test in the P-Value< 0.05. The results illustrated that essential oil in dilution 20% has more inhibitor activity against 20 Strains of Candida albicans. The results of the present project indicate that the Achillea wilhelmsii flowers inhibited fungi growth but their effectiveness varied. Therefore we will be able perform researches with extraction of this plant effective compound for the treantment of infectious disease.