Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

1,647
Articles
Q3 0.80
Impact Factor
Q3 1.3
CiteScore
7 days
Avg. First Decision
Outline
Figures and Tables
ARTICLE METRICS
Views 858
Downloads 289

Extracellular Amylase Activity from Endophytic Streptomyces griseoflavus P4

Julaluck Tang-um and Hataichanoke Niamsup*
* Author for corresponding; e-mail address: hataichanoke.n@cmu.ac.th
Volume: Vol.39 No.2 (APRIL 2012)
Research Article
DOI:
Received: 8 August 2011, Revised: -, Accepted: 16 November 2011, Published: -

Citation: Tang-um J. and Niamsup H., Extracellular Amylase Activity from Endophytic Streptomyces griseoflavus P4, Chiang Mai Journal of Science, 2012; 39(2): 346-350.

Abstract

Amylase enzymes are currently used to accelerate starch degradation in many industrial processes. Streptomyces sp., an endophytic actinomycete, is well known as a potential source of hydrolytic enzymes, antimicrobial agents, and many secondary metabolites. Previously, an endophytic Streptomyces griseoflavus P4 was isolated from sweet pea root and identified by 16S rRNA sequence analysis. Using a plate assay, the S. griseoflavus P4 was found to be capable of producing amylase enzymes, as a clear zone around colonies appeared after iodine reagent was added to the plate. The maximum amylase activity was 1.66±0.07 U/mL on the 7th day of growth, while the total biomass was 0.085±0.005 g of dry pellet in 100 mL medium. In this work, the P4 strain exhibited a specific activity of 118.4 U/mg protein. Amylase production was found to be growth-associated and started in a log phase.  Maximum enzyme production was reached during the stationary phase. The optimal conditions for activity were found to be 40 degrees C and a pH of 7.

Keywords: Endophyte, actinomycete, streptomycete, amylolytic activity, starch

Related Articles

Production of Coating from Rice Starch and Cassia alata Linn. Leaf Extract for Ripening Delay in ‘Namwa’ (ABB) Banana Fruit
DOI: 10.12982/CMJS.2025.055.

Pawinee Theamdee, Wutthichai Sriwichai and Janejira Detraksa

Vol.52 No.4 (July 2025)
Research Article View: 492 Download: 222
The Effect of Whey Protein Concentrate Addition on the Gel Properties of Potato Starch and the Quality of Vermicelli
DOI: 10.12982/CMJS.2024.042.

Yan Li, Shuxing Chen, Peng Fei and Qianwen Li

Vol.51 No.3 (May 2024)
Research Article View: 773 Download: 523
Characterization of Grain Quality Traits of Thai Highland Glutinous Rice Landraces
DOI: 10.12982/CMJS.2024.007.

Siriluk Toosang, Sansanee Jamjod and Tonapha Pusadee

Vol.51 No.1 (January 2024)
Research Article View: 1,952 Download: 1,118
Screening of Cellulose and Pectin Degrading Actinomycetes and Evaluation of the Ability of Composite Flora to Degrade Corn Straw
DOI: 10.12982/CMJS.2023.021.

Xiaofei Liu, Jie Lian, Zhihui Zheng, Yuena Qi, Guang Zhang, Wei Wang and Na Zhang

Vol.50 No.3 (May 2023)
Research Article View: 1,667 Download: 629
Effect of Pregelatinized Starch on the Properties of Poly (Vinyl Alcohol) Film for the Water-Soluble Laundry Bag
DOI: 10.12982/CMJS.2022.096.

Montree Sangthongdee, Supanut Phattarateera and Poonsub Threepopnatkul

Vol.49 No.6 (November 2022)
Research Article View: 1,066 Download: 792
Effect of Microwave and Infrared Heating Process on Increasing Resistant Starch type 3 and Reducing Glycemic Index in RD 43 Rice
DOI: 10.12982/CMJS.2022.040.

Nattarat Techanet, Arthitaya Kawee-ai, Natcha Laokuldilok and Niramon Utama-ang

Vol.49 No.2 (March 2022)
Research Article View: 2,028 Download: 1,360
Effects of Endophytes on Dysphania ambrosioides Heavy Metal Tolerance and Accumulation under Multi-heavy Metal Stress
DOI: 10.12982/CMJS.2022.022.

Wenting Tang, Yonghan Gao, Qiaohong Li, Wenqin Mao, Yuejuan Jiang and Haiyan Li

Vol.49 No.2 (March 2022)
Research Article View: 1,771 Download: 674
Effect of Rice Seeds Germination Bioprimed with Actinomycetes Isolated from Peat Swamp Forest
page: 996 - 1008

Wee Fei Aaron Teo, Supattra Muangham, Kenika Lipun and Kannika Duangmal

Vol.48 No.4 (July 2021)
Research Article View: 1,641 Download: 1,461
Semi-dry Impregnation Method of Rice Starch with Xanthan Gum Prior to Modification by Dry Heat Treatment
page: 429 - 443

Jian Su, Bhakkhawat Laoka, Pathama Chatakanonda, Wirat Vanichsriratana, Klanarong Sriroth, Sirithorn Lertphanich, Sunee Chotineeranat and Kuakoon Piyachomkwan

Vol.48 No.2 (March 2021)
Research Article View: 987 Download: 694
Effect of Different Crosslinking Agents on Properties of Dual Modified Starch Biodegradable Films Prepared by Crosslinked-Oxidized Method
page: 1216 - 1229

Yossathorn Tanetrungroj and Jutarat Prachayawarakorn*

Vol.47 No.6 (November 2020)
Research Article View: 985 Download: 1,833
Isolation and Selection of Plant Growth Promoting Endophytic Bacteria Associated with Healthy Hevea brasiliensis for Use as Plant Growth Promoters in Rubber Seedlings under Salinity Stress
page: 39 - 48

Kulwadee Khotchanalekha, Weerasak Saksirirat, Supat Isarangkool Na Ayutthaya, Kenji Sakai, Yukihiro Tashiro, Yuki Okugawa and Saowanit Tongpim

Vol.47 No.1 (January 2020)
Research Article View: 1,171 Download: 681
Diversity of culturable endophytic fungi associated with bryophytes, pteridophytes and spermatophytes from Dawei Mountain Nature Reserve, China
page: 626 - 638

Yong-Han Gao, Wei-Xiao Bai, Wei-Hong Sun, Wen-Na Zhou, Guang-Li Wu, Zheng-Qing Zhu, Jia-Yun Ma, Hai-Yan Li*

Vol.46 No.4 (July 2019)
Research Article View: 1,058 Download: 377
Potential of Selected Mangrove Streptomyces as Plant Growth Promoter and Rice Bakanae Disease Control Agent
page: 261 - 276

Wasu Pathom-aree, Satidaporn Kreawsa, Manita Kamjam, Shinji Tokuyama, Sada Yoosathaporn and Saisamorn Lumyong

Vol.46 No.2 (March 2019)
Research Article View: 1,118 Download: 429
Synthesis of Starch Derived Sulfonated Carbon-based Solid Acid as a Novel and Efficient Nanocatalyst for the Synthesis of Dihydropyrimidinones
page: 132 - 143

Zahra Hoseinabadi and Seied A. Pourmousavi

Vol.46 No.1 (January 2019)
Research Article View: 983 Download: 398
Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)
page: 2652 - 2665

Ratchanee Mingma and Kannika Duangmal

Vol.45 NO.7 (November 2018)
Research Article View: 1,078 Download: 435
Characterization of Crystalline Structure and Thermostability of Debranched Chickpea Starch-Lauric Acid Complexes Prepared Under Different Complexation Conditions
page: 1796 - 1810

Suchitra Wongprayoon, Thierry Tran, Olivier Gibert, Eric Dubreucq, Kuakoon Piyachomkwan and Klanarong Sriroth

Vol.45 No.4 (July 2018)
Research Article View: 1,124 Download: 1,265
Molecular Cloning and Sequencing of Raw Starch Degrading Gene from Laceyella sacchari LP175 and Its Functional Expression in Escherichia coli
page: 1634 - 1648

Thanasak Lomthong, Sunee Chotineeranat, Gianluca Cioci, Elisabeth Laville, Sophie Duquesne, Kiattawee Choowongkomon, Alain Marty and Vichien Kitpreechavanich

Vol.45 No.4 (July 2018)
Research Article View: 1,406 Download: 465
Isolation and Diversity of Actinomycetes from Sediments of Different Depths Between 34 m and 3,235 m in South China Sea
page: 1595 - 1609

Manita Kamjam, Qinqyi Xie, Zixin Deng and Kui Hong

Vol.45 No.4 (July 2018)
Research Article View: 1,415 Download: 426
A Complete System for Renewable Utilization of Cassava to Biohydrogen Coupled with Biomethane as Zero-Waste Management
page: 1214 - 1224

Wilawan Channarong, Mongkol Ngamjarearnwong, Churapa Teerapatsakul and Lerluck Chitradon*

Vol.45 No.3 (May 2018)
Research Article View: 1,028 Download: 288
Antioxidant Activity and Starch-digesting Enzyme Inhibition of Selected Thai Herb Extracts
page: 263 - 276

Krittalak Pasakawee, Somdet Srichairatanakool, Thunnop Laokuldilok and Niramon Utama-ang

Vol.45 No.1 (January 2018)
Research Article View: 1,029 Download: 288
Simultaneous Saccharification and Fermentation of L-(+)-Lactic Acid Production from Liquefied Cassava Starch by Immobilized Rhizopus oryzae in a 3 L Airlift Fermenter
page: 77 - 91

Srisakul Trakarnpaiboon, Suthasinee Praneetrattananon and Vichien Kitpreechavanich

Vol.45 No.1 (January 2018)
Research Article View: 1,342 Download: 548
Diversity and Plant Growth Promoting Activities of Actinomycetes from Mangroves
page: 1210 - 1223

Paweena Suksaard, Wasu Pathom-aree and Kannika Duangmal

Vol.44 No.4 (October 2017)
Research Article View: 1,591 Download: 566
In vitro Antimicrobial and Cytotoxic Activities of Mangrove Actinomycetes from Eastern Thailand
page: 322 - 337

Janpen Tangjitjaroenkun [a], Rungnapa Tangchitcharoenkhul [b], Waraporn Yahayo [c] and Roongtawan Su

Vol.44 No.2 (April 2017)
Research Article View: 970 Download: 228
Diversity of Endophytic Actinomycetes Isolated from Roots and Root Nodules of Pueraria candollei Grah. ex Benth. and the Analyses of Their Secondary Metabolites
page: 1 - 14

Panitch Boonsnongcheep [a], Takuji Nakashima [b], Yōko Takahashi [b] and Sompop Prathanturarug* [a]

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,070 Download: 403
Facile, Versatile and Green Synthesis of Silver Nanoparticles by Mung Bean Starch and Their Catalytic Activity in the Reduction of 4-Nitrophenol
page: 610 - 620

Sanoe Chairam *[a] and Ekasith Somsook [b]

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,125 Download: 495
Earthy-musty Odour and Off-flavour Taints in Phayao Lake, Thailand
page: 22 - 31

Supannee Suwanpakdee, Redel Gutierrez, Santiwat Pithakpol, Arunothai Jampeetong, Wasu Pathom-aree, Nakao Nomura, Tomoaki Itayama[f] and Niwooti Whangchai

Vol.43 No.1 (JANUARY 2016)
Research Article View: 977 Download: 398
Effect of Pectin Contents on Properties of Biodegradable Thermoplastic Mung Bean Starch/low-density Polyethylene Blends Using Injection Molding Technique
page: 215 - 225

Jutarat Prachayawarakorn*[a,b] and Luxana Hommanee [a]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 938 Download: 292
Selective Isolation of Cultivable Actinomycetes from Thai Coastal Marine Sediment
page: 88 - 103

Pornpan Ruttanasutja and Wasu Pathom-aree

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,283 Download: 317
Production of Probiotics Streptomyces Biomass from Starchy Wastewater
page: 294 - 298

Suteera Srisamai, Phonlawat Srikhampa, and Wasu Pathom-aree*

Vol.40 No.2 (APRIL 2013)
Research Article View: 1,325 Download: 273
Screening of Volcanic Cave Actinomycetes for Antimicrobial Activity Against Paenibacillus larvae, A Honey Bee Pathogen
page: 26 - 33

Silvia Kay [a], Wasu Pathom-aree [b], and Naowarat Cheeptham*[a]

Vol.40 No.1 (JANUARY 2013)
Research Article View: 982 Download: 362
Actinomycetes from Tropical Limestone Caves
page: 373 - 388

Nanthavut Niyomvong, Wasu Pathom-aree, Arinthip Thamchaipenet andKannika Duangmal

Vol.39 No.3 (JULY 2012)
Research Article View: 915 Download: 438
Outline
Figures