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Proteomic Analysis of Bradyrhizobium japonicum USDA110 in Acidic Condition
Paper Type  |
Contributed Paper |
Title |
Proteomic Analysis of Bradyrhizobium japonicum USDA110 in Acidic Condition |
Author |
Napat Puranamaneewiwat [a,b] Shigeyuki Tajima [c] and Hataichanoke Niamsup* [b] |
Email |
scchi029@chiangmai.ac.th |
|
Abstract: Bradyrhizobium japonicum is an agriculturally important bacterium due to its capacity of nitrogen-fixing symbiosis with leguminous plants, especially soybean (Glycine max). In this report, we studied acid-tolerance metabolism of B. japonicum USDA110 using proteome analysis. Two-dimensional electrophoresis gel image analysis revealed 568 and 628 protein spots of cells grown at pH 4.7 and pH 6.8, respectively. Only 84 protein spots with at least 3-fold differential expression were further identified by MALDI-TOF MS. The annotated proteins were assigned to four different classes: (i) proteins produced only at pH 4.7 condition (15 proteins such as D-alanine aminotransferase, 2-haloalkanoic acid dehalogenase and periplasmic mannitol-binding protein); (ii) proteins produced under both conditions but strongly induced
at pH 4.7 (27 protein spots such as triosephosphate isomerase, UTP-glucose-1 phosphate uridylyltransferase and glyceraldehyde 3-phosphate dehydrogenase); (iii) proteins down-regulated during growth at pH 4.7 (25 proteins such as GroEL, acyl-CoA dehydrogenase and ATP synthase beta chain), and (iv) proteins specific to growth at pH 6.8 (17 proteins such as ATPdependent protease ATP-binding subunit, N-utilization substance protein A and 2- isopropylmalate synthase). The data of the differential protein expression can be a basis for mechanism elucidation of the acid response in B. japonicum USDA110. |
|
Start & End Page |
335 - 345 |
Received Date |
2006-01-01 |
Revised Date |
|
Accepted Date |
2006-05-03 |
Full Text |
Download |
Keyword |
acidic condition, Bradyrhizobium japonicum, proteomic, rhizobium, two-dimensional gel electrophoresis |
Volume |
Vol.33 No.3 (SEPTEMBER 2006) |
DOI |
|
Citation |
[a N.P., [c] B.S.T. and [b] H.N., Proteomic Analysis of Bradyrhizobium japonicum USDA110 in Acidic Condition, Chiang Mai Journal of Science, 2006; 33(3): 335-345. |
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