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JOURNAL DETAIL
Diversity of Bacteria Nodulating Medicago arborea in the Northeast Area of Morocco
Paper Type  |
Contributed Paper |
Title |
Diversity of Bacteria Nodulating Medicago arborea in the Northeast Area of Morocco |
Author |
Kamal Guerrouj* [a], Mustapha Bouterfas[b], Hanaa Abdelmoumen [c] and Mustapha Missbah El Idrissi [c] |
Email |
kamaleddine.guerrouj@yahoo.fr |
|
Abstract: A Polyphasic characterization of 30 bacteria isolated from root nodules of Medicago arborea (Medic tree) plants growing in 3 arid soils of the arid northeast region of Morocco was performed. The phenotypic, symbiotic, and cultural characteristics analyzed allowed the description of a broad physiological diversity among the isolates. The phenogram obtained showed 3 main clusters at a similarity coefficient of 78%. The results obtained suggest that the phenotype of these rhizobia might have evolved for adaptation to the local conditions.
The genetic characterization which consisted in a rep PCR using ERIC (Enterobacterial Repetitive Intergenic Consensus) primers showed a great diversity and 3 groups and 8 clusters were delimited at a similarity level of 80%. However, the 16S rDNA sequences showed a perfect identity of all the isolates with Sinorhizobium meliloti published sequences. The results showed a relationship between rep-PCR fingerprinting and phenotypic techniques, which are complementary in diversity investigation and allowed revelation of relationships between M. arborea close-related bacterial isolates originated from different geographic location in the northeast area of Morocco. |
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Start & End Page |
441 - 452 |
Received Date |
2014-03-28 |
Revised Date |
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Accepted Date |
2015-06-16 |
Full Text |
Download |
Keyword |
Medicago arborea, rhizobium, 16S rDNA, bacterial diversity, ERIC-PCR |
Volume |
Vol.43 No.3 (APRIL 2016) |
DOI |
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SDGs |
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