Isolation and Characterization of Brassinolide and Castasterone in the Pollen of Pumpkin
Chalev Pachthong[a,b], Dumrat Supyen[a], Duang Buddhasukh*[a] and Araya Jatisatienr[c]* Author for corresponding; e-mail address: duang@chiangmai.ac.th
Volume: Vol.33 No.1 (JANUARY 2006)
Research Article
DOI:
Received: 15 September 2005, Revised: -, Accepted: 29 September 2005, Published: -
Citation: Pachthong C.., Supyen D.. and Jatisatienr D..B..A.., Isolation and Characterization of Brassinolide and Castasterone in the Pollen of Pumpkin, Chiang Mai Journal of Science, 2006; 33(1): 95-101.
Abstract
The two brassinosteroids contained in the pollen of pumpkin (Cucurbita moschata Duch.) were investigated. The bioactive compounds from the purified extract were identified by GC-MS as brassinolide and castasterone. The content of brassinolide and castasterone were estimated to be 36 µg kg-1 and 112 µg kg-1 (dry weight) respectively. This study provides the first evidence for the occurrence of brassinolide and castasterone in the pollen of pumpkin.