Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Choanephora infundibulifera Associated with Fruit Rot on Butternut Squash (Cucurbita moschata) in Thailand, and its In Vitro Response to a Biological Control Agent and Fungicides

Chaninun Pornsuriya, Samak Kaewsuksaeng, Nutthachai Pongprasert, Prisana Wonglom* and Anurag Sunpapao*
* Author for corresponding; e-mail address: anurag.su@psu.ac.th, prisana.w@tsu.ac.th
ORCID ID: https://orcid.org/0000-0002-3208-4056
Volume: Vol.53 No.3 (May 2026)
Research Article
DOI: https://doi.org/10.12982/CMJS.2026.041
Received: 29 October 2025, Revised: 27 March 2026, Accepted: 30 March 2026, Published: -

Citation: Pornsuriya C., Kaewsuksaeng S., Pongprasert N., Wonglom P. and Sunpapao A., Choanephora infundibulifera associated with fruit rot on butternut squash (Cucurbita moschata) in Thailand, and its in vitro response to a biological control agent and fungicides. Chiang Mai Journal of Science, 2026; 53(3): e2026041. DOI 10.12982/CMJS.2026.041.

Graphical Abstract

Graphical Abstract

Abstract

     Butternut squash (Cucurbita moschata) is susceptible to fruit rot under tropical conditions. In 2025, a fruit rot disease was observed on butternut in Songkhla Province, southern Thailand. The causal agent was identified as Choanephora infundibulifera based on morphological characteristics, internal transcribed spacer (ITS) and large subunit (LSU) sequence analyses, and pathogenicity tests fulfilling Koch’s postulates. This represents the first report of C. infundibulifera causing fruit rot on butternut in Thailand. In vitro assays demonstrated that the biological control agent Trichoderma breve Z2-03 significantly inhibited mycelial growth of five C. infundibulifera isolates, with inhibition ranging from 42.68% to 60.71%. Synthetic fungicides, including captan, carbendazim, mancozeb, and propiconazole, were highly effective, suppressing fungal growth by more than 80% across all isolates. These findings highlight the potential of both biological and chemical approaches for managing this emerging pathogen. The occurrence of C. infundibulifera on butternut suggests an increasing risk of disease emerging under tropical and climate-driven conditions. This study not only reports a new host–pathogen association but also provides practical baseline data for developing integrated disease management strategies for butternut and related crops.

Keywords: biological control, Choanephora, Cucurbita moschata, fruit rot, fungicide, morphology, PCR, phylogeny

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